Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 10,965 results for: T7

Showing: 9681 - 9700 of 10965 results

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pSG086

Plasmid

#214263

Purpose

PaFtsH2H1-link-32-6xHis: PaftsH2(ftsH2_F124-V154del_ins ftsH1_M120-T151)-6xHis cloned in XbaI/NheI site in pET-28a(+)

Depositor

Ute Romling

Article

Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

Insert

Hybrid PaFtsH protease PaFtsH2H1-link-32 of Pseudomonas aeruginosa SG17M

Tags

6x His-tag

Expression

Bacterial

Mutation

substitution of 31 amino acids of FtsH2 (F124RRFA…

Promoter

T7 promoter

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET21a Di-iPGM

Plasmid

#180241

Purpose

Bacterial expression plasmid for production of recombinant Dirofilaria immitis iPGM His10

Depositor

James Inglese

Article

Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

Insert

Dirofilaria immitis iPGM-10His

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanII-2C

Plasmid

#225070

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2C (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2C)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanII-2A

Plasmid

#225068

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2A (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2A)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanII-2B

Plasmid

#225069

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2B (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2B)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanII-57

Plasmid

#225074

Purpose

Expresses two precursor peptides and the lanthipeptide synthetase from LanII-57 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA(A) (LanII-57)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanIII-6

Plasmid

#225076

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanIII-6 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA(A) (LanIII-6)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanII-23

Plasmid

#225067

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-23 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-23)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1:mTRESK(S116C)

Plasmid

#224772

Purpose

In vitro transcription for Oocyte expression of mTRESK(S116C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 18 (Kcnk18 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S116C

Promoter

T7

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:hTRAAK(S118C/Q258K)

Plasmid

#224764

Purpose

In vitro transcription for Oocyte expression of hTRAAK(S118C/Q258K)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 4 (KCNK4 Human)

Use

In vitro transcription for oocyte expression

Mutation

S118C/Q258K

Promoter

T7

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:hTRAAK(Q258K)

Plasmid

#224763

Purpose

In vitro transcription for Oocyte expression of hTRAAK(Q258K)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 4 (KCNK4 Human)

Use

In vitro transcription for oocyte expression

Mutation

Q258K

Promoter

T7

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1

Plasmid

#224743

Purpose

In vitro transcription for Oocyte expression of mTREK-1

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Promoter

T7

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(S131C)

Plasmid

#224744

Purpose

In vitro transcription for Oocyte expression of mTREK-1(S131C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S131C

Promoter

T7

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTRAAK(S93C/Q233K)

Plasmid

#224768

Purpose

In vitro transcription for Oocyte expression of mTRAAK(S93C/Q233K)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 4 (Kcnk4 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S93C / Q233K

Promoter

T7

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(S131A)

Plasmid

#224745

Purpose

In vitro transcription for Oocyte expression of mTREK-1(S131A)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S131A

Promoter

T7

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTRAAK(Q233K)

Plasmid

#224767

Purpose

In vitro transcription for Oocyte expression of mTRAAK(Q233K)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 4 (Kcnk4 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

Q233K

Promoter

T7

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:hTRAAK(S118C)

Plasmid

#224762

Purpose

In vitro transcription for Oocyte expression of hTRAAK(S118C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 4 (KCNK4 Human)

Use

In vitro transcription for oocyte expression

Mutation

S118C

Promoter

T7

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 TREK-2(S156C)

Plasmid

#224770

Purpose

In vitro transcription for Oocyte expression of 1 TREK-2(S156C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 10 (Kcnk10 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S156C

Promoter

T7

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(G171F)

Plasmid

#224747

Purpose

In vitro transcription for Oocyte expression of mTREK-1(G171F)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

G171F

Promoter

T7

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTRAAK(S93C)

Plasmid

#224766

Purpose

In vitro transcription for Oocyte expression of mTRAAK(S93C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 4 (Kcnk4 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S93C

Promoter

T7

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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