...insoluble, or membrane proteins  T7 Express   IPTG-inducible expression of T7 RNAP from the genome; does not...lysogen/T7 promoter combination is the most popular induction system. The DE3 lysogen expresses T7 RNA polymerase...addition of IPTG. T7 RNAP is then available to transcribe the gene of interest from a T7 promoter on the...BL21 (DE3)   Basic IPTG-inducible strain containing T7 RNAP (DE3) F- ompT lon hsdSB (rB- mB-) gal dcm (...DE3) pLysS* Chloramphenicol (pLysS) pLysS expresses T7 lysozyme to reduce basal expression levels; expression...) pLysE* Chloramphenicol (pLysE) pLysE has higher T7 lysozyme expression than pLysS; expression vector...A1 Tetracycline Arabinose-inducible expression of T7 RNAP; IPTG may still be required for expression ...

...of a 40 kb T7 phage genome. They first compared 10-piece linear (the native state of the T7 genome) and...the same tools, they successfully assembled a 40 kb T7 phage genome from 52 (!!) fragments in one very Golden...ligase was the correct choice for the job compared to T7 ligase (it was—see Potapov et al., 2018). More complex...linear ones. The 52-piece circular assembly of the T7 genome had lower efficiency than the 10-piece circular...

...containing the T7 RNA polymerase gene or using cells that stably express T7 (e.g. BSR T7/5 cells) (1). ...minigenome) are under control of a T7 RNA polymerase promoter sequence. Thus, T7 RNA polymerase also needs to...

...Additional considerations T7 in vitro transcription/ general expression Promoter from T7 bacteriophage Constitutive...from T7 bacteriophage plus lac operators Negligible basal expression when not induced. Requires T7 RNA ...Constitutive, but requires T7 RNA polymerase. When used for in vitro transcription, the promoter drives either...

...Briefly, you design your donor template to include a T7 promoter upstream of the left homology arm, perform...transcription to create your ssDNA. Note that the T7 promoter will not be included in the final ssDNA ...(i.e. GG); this design has been shown to increase T7 promoter activity. To avoid re-cutting by Cas9 after...containing the homology arms on both sides and the T7 promoter on one end. Typically, Easi-CRISPR works...blue flame plasmid pmScarlet_C1! After creating the T7-DNA template, you'd use ivTRT to create the ssDNA...using Addgene plasmids. Design PCR primers to add T7 promoter-left homology arm and right homology arm...

...lab’s new plasmid, T444T, contains T7 terminators adjacent to the T7 promoters to prevent the incorporation... L4440, places a target gene between two inverted T7 promoters. Transcription from each promoter progresses...

...expresses gene III. Gene III is under the control of a T7 RNA polymerase and cannot be expressed until the ...the base editor corrects early stop codons within T7 RNAP. A phage encoding the deaminase TadA-7.10 A mutagenesis...

...this plasmid harbors a multi-cloning site flanked by T7 promoters to allow for bidirectional transcription...expression is activated through the  induction of T7 polymerase encoded in the E. coli genome (usually...

...produce molecular biological tools like Taq polymerase, T7 polymerase, EcoRI, and various fluorescent proteins...

...VCP His T7 ALS Axel Brunger 12339 pBD-0071 VCP His T7 ALS Axel Brunger 12344 pBD-0010 VCP His T7 ALS Axel...VCP His T7 ALS Axel Brunger 12346 pBD-0013 VCP His T7 ALS Axel Brunger 12347 pBD-0006 VCP His T7 ALS Axel...VCP His T7 ALS Axel Brunger 12349 pBD-0070 VCP His T7 ALS Axel Brunger 12350 pBD-0069 VCP His T7 ALS Axel...VCP His T7 ALS Axel Brunger 12352 pBD-0007 VCP His T7 ALS Axel Brunger 12353 pBD-0018 VCP His T7 ALS Axel...VCP His T7 ALS Axel Brunger 12355 pBD-0022 VCP His T7 ALS Axel Brunger 12356 pBD-0024 VCP His T7 ALS Axel...VCP His T7 ALS Axel Brunger 12358 pBD-0035 VCP His T7 ALS Axel Brunger 12359 pBD-0039 VCP His T7 ALS Axel...VCP His T7 ALS Axel Brunger 12361 pBD-0072 VCP His T7 ALS Axel Brunger 12362 pBD-0050 VCP His T7 ALS Axel...

...protein of interest Grow fusion protein plasmid in NEB T7 SHuffle bacteria (C3029J). We are clearly NEB “fanboys...

...Gate TALEN 2.0 37184 pCAG-T7-TALEN(Sangamo)-Destination Pawel Pelczar pCAG-T7-TALEN(Sangamo)-Destination...FokI domains. 40131 pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination 40132 pCAG-T7-TALEN(Sangamo)-FokI-ELD-...can be achieved by in vitro mRNA synthesis from the T7 promoter. Truncations were introduced to the N- and... cleavage activity in several later studies. pCAG-T7-TALEN(Sangamo)-Destination vectors are available ...double-transfected mammalian cells. In addition, a T7 promoter was included for generating TALEN mRNAs ...

...thioredoxin-binding domain (TBD) from bacteriophage T7 DNA polymerase increased the processivity of PolI3M...

...expression plasmids in a high copy backbone with a T7 promoter driving multiple combinations of WT, codon...

...sgRNA) R07E5.16 U6 yes, cut Goldstein 42250 DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid...Transfection none, need Cas9 plasmid Boxem 47941 pMB60 T7 BsaI In vitro transcription none, need Cas9 plasmid...Cas9 plasmid Sternberg 177783 L4440_BioBrick-sgRNA T7 BbsI, BsaI Ingestion by C. elegans none, need Cas9...

...Of all the vectors, pET, under the control of the T7 lac promoter and induced by lactose, provides the...

... include naturally occurring terminators, such as T7 and rrnB, as well as engineered high-efficiency terminators...

...pT7-gRNA T7 BsmBI In vitro transcription none, need Cas9 plasmid Chen and Wente 42250 DR274 T7 BsaI In ...

...pOpen-TthpolA Tth DNA polymerase 165534 pOpen-T7gene5 T7 DNA Polymerase (unmodified) 165542 pOpen-KTHEAQpol...Addgene ID Plasmid Gene/insert 165505 pOpen-T7gene1 T7 RNA Polymerase 165516 pOpen-SP6rpol SP6 RNA Polymerase...glycosylase, aka T4 Endonuclease V) 165540 pOpen-T7EI T7 Endonuclease I 165551 pOpen-Endonuclease IV E. coli...

...The pMAK461-64 plasmids were expressed in SHuffle T7 Express competent E.coli (NEB) using IPTG induction...