...insoluble, or membrane proteins  T7 Express   IPTG-inducible expression of T7 RNAP from the genome; does not...lysogen/T7 promoter combination is the most popular induction system. The DE3 lysogen expresses T7 RNA polymerase...addition of IPTG. T7 RNAP is then available to transcribe the gene of interest from a T7 promoter on the...BL21 (DE3)   Basic IPTG-inducible strain containing T7 RNAP (DE3) F- ompT lon hsdSB (rB- mB-) gal dcm (...DE3) pLysS* Chloramphenicol (pLysS) pLysS expresses T7 lysozyme to reduce basal expression levels; expression...) pLysE* Chloramphenicol (pLysE) pLysE has higher T7 lysozyme expression than pLysS; expression vector...A1 Tetracycline Arabinose-inducible expression of T7 RNAP; IPTG may still be required for expression ...

...of a 40 kb T7 phage genome. They first compared 10-piece linear (the native state of the T7 genome) and...the same tools, they successfully assembled a 40 kb T7 phage genome from 52 (!!) fragments in one very Golden...ligase was the correct choice for the job compared to T7 ligase (it was—see Potapov et al., 2018). More complex...linear ones. The 52-piece circular assembly of the T7 genome had lower efficiency than the 10-piece circular...

...containing the T7 RNA polymerase gene or using cells that stably express T7 (e.g. BSR T7/5 cells) (1). ...minigenome) are under control of a T7 RNA polymerase promoter sequence. Thus, T7 RNA polymerase also needs to...

...Additional considerations T7 in vitro transcription/ general expression Promoter from T7 bacteriophage Constitutive...from T7 bacteriophage plus lac operators Negligible basal expression when not induced. Requires T7 RNA ...Constitutive, but requires T7 RNA polymerase. When used for in vitro transcription, the promoter drives either...

...Briefly, you design your donor template to include a T7 promoter upstream of the left homology arm, perform...transcription to create your ssDNA. Note that the T7 promoter will not be included in the final ssDNA ...(i.e. GG); this design has been shown to increase T7 promoter activity. To avoid re-cutting by Cas9 after...containing the homology arms on both sides and the T7 promoter on one end. Typically, Easi-CRISPR works...blue flame plasmid pmScarlet_C1! After creating the T7-DNA template, you'd use ivTRT to create the ssDNA...using Addgene plasmids. Design PCR primers to add T7 promoter-left homology arm and right homology arm...

...lab’s new plasmid, T444T, contains T7 terminators adjacent to the T7 promoters to prevent the incorporation... L4440, places a target gene between two inverted T7 promoters. Transcription from each promoter progresses...

...expresses gene III. Gene III is under the control of a T7 RNA polymerase and cannot be expressed until the ...the base editor corrects early stop codons within T7 RNAP. A phage encoding the deaminase TadA-7.10 A mutagenesis...

...this plasmid harbors a multi-cloning site flanked by T7 promoters to allow for bidirectional transcription...expression is activated through the  induction of T7 polymerase encoded in the E. coli genome (usually...

...produce molecular biological tools like Taq polymerase, T7 polymerase, EcoRI, and various fluorescent proteins...

...protein of interest Grow fusion protein plasmid in NEB T7 SHuffle bacteria (C3029J). We are clearly NEB “fanboys...

...thioredoxin-binding domain (TBD) from bacteriophage T7 DNA polymerase increased the processivity of PolI3M...

...expression plasmids in a high copy backbone with a T7 promoter driving multiple combinations of WT, codon...

...Of all the vectors, pET, under the control of the T7 lac promoter and induced by lactose, provides the...

... include naturally occurring terminators, such as T7 and rrnB, as well as engineered high-efficiency terminators...

...The pMAK461-64 plasmids were expressed in SHuffle T7 Express competent E.coli (NEB) using IPTG induction...

... synthetic phages, each based on the well-studied T7 phage, to target various types of bacteria. In mixed...

...transcriptase (RT)-RPA, respectively. RPA is coupled with T7 transcription to convert amplified DNA to RNA for...

...be produced. Many commonly-used promoters, such as T7, CMV, EF1A, and SV40, are always active and thus ...

...DNA into E. coli BL21 (DE3) cells (these have the T7 polymerase and a few proteases knocked out making...

...Grunwald pCS2TAL3-DD, pCS2TAL3-RR Pawel Pelczar pCAG-T7-TALEN(Sangamo)-Destination series, pCAG-Golden-Gate-Esp3I-Destination...