Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 21 results for: T7

Showing: 1 - 20 of 21 results

  1. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...insoluble, or membrane proteins  T7 Express   IPTG-inducible expression of T7 RNAP from the genome; does not...lysogen/T7 promoter combination is the most popular induction system. The DE3 lysogen expresses T7 RNA polymerase...addition of IPTG. T7 RNAP is then available to transcribe the gene of interest from a T7 promoter on the...BL21 (DE3)   Basic IPTG-inducible strain containing T7 RNAP (DE3) F- ompT lon hsdSB (rB- mB-) gal dcm (...DE3) pLysS* Chloramphenicol (pLysS) pLysS expresses T7 lysozyme to reduce basal expression levels; expression...) pLysE* Chloramphenicol (pLysE) pLysE has higher T7 lysozyme expression than pLysS; expression vector...A1 Tetracycline Arabinose-inducible expression of T7 RNAP; IPTG may still be required for expression ...

  2. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...of a 40 kb T7 phage genome. They first compared 10-piece linear (the native state of the T7 genome) and...the same tools, they successfully assembled a 40 kb T7 phage genome from 52 (!!) fragments in one very Golden...ligase was the correct choice for the job compared to T7 ligase (it was—see Potapov et al., 2018). More complex...linear ones. The 52-piece circular assembly of the T7 genome had lower efficiency than the 10-piece circular...

  4. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...Additional considerations T7 in vitro transcription/ general expression Promoter from T7 bacteriophage Constitutive...from T7 bacteriophage plus lac operators Negligible basal expression when not induced. Requires T7 RNA ...Constitutive, but requires T7 RNA polymerase. When used for in vitro transcription, the promoter drives either...

  5. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...Briefly, you design your donor template to include a T7 promoter upstream of the left homology arm, perform...transcription to create your ssDNA. Note that the T7 promoter will not be included in the final ssDNA ...(i.e. GG); this design has been shown to increase T7 promoter activity. To avoid re-cutting by Cas9 after...containing the homology arms on both sides and the T7 promoter on one end. Typically, Easi-CRISPR works...blue flame plasmid pmScarlet_C1! After creating the T7-DNA template, you'd use ivTRT to create the ssDNA...using Addgene plasmids. Design PCR primers to add T7 promoter-left homology arm and right homology arm...

  12. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...thioredoxin-binding domain (TBD) from bacteriophage T7 DNA polymerase increased the processivity of PolI3M...

  14. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...Of all the vectors, pET, under the control of the T7 lac promoter and induced by lactose, provides the...

  15. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ... include naturally occurring terminators, such as T7 and rrnB, as well as engineered high-efficiency terminators...

  16. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...The pMAK461-64 plasmids were expressed in SHuffle T7 Express competent E.coli (NEB) using IPTG induction...

  17. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ... synthetic phages, each based on the well-studied T7 phage, to target various types of bacteria. In mixed...

  19. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...be produced. Many commonly-used promoters, such as T7, CMV, EF1A, and SV40, are always active and thus ...
Showing: 1 - 20 of 21 results