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Showing: 1 - 15 of 15 results
  1. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...VCP His T7 ALS Axel Brunger 12339 pBD-0071 VCP His T7 ALS Axel Brunger 12344 pBD-0010 VCP His T7 ALS Axel...VCP His T7 ALS Axel Brunger 12346 pBD-0013 VCP His T7 ALS Axel Brunger 12347 pBD-0006 VCP His T7 ALS Axel...VCP His T7 ALS Axel Brunger 12349 pBD-0070 VCP His T7 ALS Axel Brunger 12350 pBD-0069 VCP His T7 ALS Axel...VCP His T7 ALS Axel Brunger 12352 pBD-0007 VCP His T7 ALS Axel Brunger 12353 pBD-0018 VCP His T7 ALS Axel...VCP His T7 ALS Axel Brunger 12355 pBD-0022 VCP His T7 ALS Axel Brunger 12356 pBD-0024 VCP His T7 ALS Axel...VCP His T7 ALS Axel Brunger 12358 pBD-0035 VCP His T7 ALS Axel Brunger 12359 pBD-0039 VCP His T7 ALS Axel...VCP His T7 ALS Axel Brunger 12361 pBD-0072 VCP His T7 ALS Axel Brunger 12362 pBD-0050 VCP His T7 ALS Axel...
  2. TALEN Plasmids and Kits

    Type
    Collection
    ...Gate TALEN 2.0 37184 pCAG-T7-TALEN(Sangamo)-Destination Pawel Pelczar pCAG-T7-TALEN(Sangamo)-Destination...FokI domains. 40131 pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination 40132 pCAG-T7-TALEN(Sangamo)-FokI-ELD-...can be achieved by in vitro mRNA synthesis from the T7 promoter. Truncations were introduced to the N- and... cleavage activity in several later studies. pCAG-T7-TALEN(Sangamo)-Destination vectors are available ...double-transfected mammalian cells. In addition, a T7 promoter was included for generating TALEN mRNAs ...
  3. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...sgRNA) R07E5.16 U6 yes, cut Goldstein 42250 DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid...Transfection none, need Cas9 plasmid Boxem 47941 pMB60 T7 BsaI In vitro transcription none, need Cas9 plasmid...Cas9 plasmid Sternberg 177783 L4440_BioBrick-sgRNA T7 BbsI, BsaI Ingestion by C. elegans none, need Cas9...
  4. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...pT7-gRNA T7 BsmBI In vitro transcription none, need Cas9 plasmid Chen and Wente 42250 DR274 T7 BsaI In ...
  5. Open Enzyme Collection

    Type
    Collection
    ...pOpen-TthpolA Tth DNA polymerase 165534 pOpen-T7gene5 T7 DNA Polymerase (unmodified) 165542 pOpen-KTHEAQpol...Addgene ID Plasmid Gene/insert 165505 pOpen-T7gene1 T7 RNA Polymerase 165516 pOpen-SP6rpol SP6 RNA Polymerase...glycosylase, aka T4 Endonuclease V) 165540 pOpen-T7EI T7 Endonuclease I 165551 pOpen-Endonuclease IV E. coli...
  6. Bacterial Expression Systems

    Type
    Collection
    ...variety of tags can be found here . pNH-TrxT 26106 T7 LacO (lactose/IPTG inducible) N terminal, TEV cleavable...protein purification. pET LIC Series Various Plasmids T7-lacO (lactose/IPTG inducible) Flag, His, Strep, mOCR... useful for protein purification. pET15-MHL 26092 T7-lacO (lactose/IPTG inducible) C-terminal TEV cleavable...protein with a 6x His tag. Requires a strain with T7 RNA polymerase. Amp resistant. More expression vectors...gram-negative hosts. pCri System Various Plasmids T7/lacO and lacO variants that can be expressed in B...
  7. Cre-lox system

    Type
    Collection
    ... variant T7 Bacterial Liu 62657 pAYC iCre CAG Mammalian Ohtsuka 62730 CRE recombinase Cre T7 Bacterial...highly cationic GFP variant T7 Bacterial Liu 62939 pET-Cre-6xHis Cre-6xHis T7 Bacterial Liu 63704 pRetroX...123128 pET-His-Cre 6xHis-Cre T7 Bacterial Liu 123131 pET-His-VCre 6xHis-VCre T7 Bacterial Liu 123133 pCMV-Cre...119868 pGEM-NLS-Cre-2A-mCherry-FKF NLS-Cre, mCherry T7 Bacterial Sauka-Spengler 119971 GAG-CRErec GAG-Cre...
  8. TALEN Expression Vectors

    Type
    Collection
    ... CMV promoter for mammalian cell expression and a T7 promoter for in vitro transcription of RNA. TALENs...
  9. Depositor Collections

    Type
    Collection
    ...MORF) Collection Synthetic Biology Voigt Fragmented T7 RNAP Plasmids Repressor Plasmids Sigma (σ) Factor...
  10. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...pyogenes (PAM = NGG) 51306 pUC57-Simple-gRNA backbone T7 BsaI In vitro transcription none, need Cas9 plasmid...
  11. Luciferase Plasmid Collection

    Type
    Collection
    ...firefly luciferase James Wilson 101156 T7-CMVtrans-FFLuc-polyA Firefly T7 Expression of firefly luciferase ...luciferase. Renilla luciferase is expressed from a T7 promoter for normalization. FMDV StopGo sequences...
  12. FlyCRISPR

    Type
    Collection
    ... pMB60 allows in vitro production of RNA from the T7 promoter, while pMB70 is designed for in vivo transcrition...
Showing: 1 - 15 of 15 results