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pIVEX2.3-CS1M2b-3c-ON8-EGFP

Plasmid

#228452

Purpose

LS4-responsive riboswitch

Depositor

Keisuke Fukunaga

Article

Fukunaga et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

CS1M2b-3c-ON8 riboswitch-regulated EGFP

Use

Synthetic Biology

Promoter

T7

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pIVEX2.3-CS2-3c-ON8-EGFP

Plasmid

#228453

Purpose

LS12-responsive riboswitch

Depositor

Keisuke Fukunaga

Article

Fukunaga et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

CS2-3c-ON8 riboswitch-regulated EGFP

Use

Synthetic Biology

Promoter

T7

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA47_dCas9-GFP_AAN_4

Plasmid

#231415

Purpose

This AND-AND-NOT-gate plasmid expresses dCas9 from a constitutive promoter. Its sgRNAs X & Y are repressible by sgRNAs A & B, respectively. Its GFP gene is repressible by any of sgRNAs X, Y, and C.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

dCas9
GFP
sgRNA-X
sgRNA-Y

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSJ107-2x

Plasmid

#208812

Purpose

Expresses SoxS activation domainand gRNA targeting two copies of 107 region within the synthetic promoter in bacterial cell

Depositor

Marc Erhardt

Article

Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:

Insert

sgRNA 107-2x_mRFP

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

CRISPR/dCas9-responsive synthetic promoter

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pSJ105

Plasmid

#208859

Purpose

Expresses SoxS activation domain and gRNA targeting 105 region within the synthetic promoter in bacterial cell

Depositor

Marc Erhardt

Article

Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:

Insert

sgRNA 105

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

CRISPR/dCas9-responsive synthetic promoter

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pSJ106-2x

Plasmid

#208860

Purpose

Expresses SoxS activation domain and gRNA targeting two copies of 106 region within the synthetic promoter in bacterial cell

Depositor

Marc Erhardt

Article

Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:

Insert

sgRNA 106-2x_mRFP

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

CRISPR/dCas9-responsive synthetic promoter

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLigify_dRibose

Plasmid

#215561

Purpose

Expresses GFP in response to D-Ribose

Depositor

Simon d'Oelsnitz, David Ross

Article

d'Oelsnitz et al ACS Synth Biol. 2024 Aug 16;13(8):2577-2

Inserts

GFP mut2
LcLacI

Expression

Bacterial

Promoter

P250 and Plclaci

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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GB4666

Plasmid

#215271

Purpose

pUPD2 for the Arabidopsis thaliana ABA-responsive MAPKKK18 promoter.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

promMAPKKK18

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2-DR

Plasmid

#216266

Purpose

Dox-responsive module fused with EGFP

Depositor

Susumu Tomita

Article

Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

Insert

Codon-optimized TetR - Lipid binding motif (KRf - KLK) - Codon-optimized TetR

Tags

EGFP

Expression

Mammalian

Available Since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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Salsa-BC293-P4B1

Plasmid

#203134

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC293

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC293

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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Salsa-BC170-P2G2

Plasmid

#203011

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC170

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC170

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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Salsa-BC157-P2F1

Plasmid

#202998

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC157

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC157

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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Salsa-BC120-P2B12

Plasmid

#202961

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC120

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC120

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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Salsa-BC121-P2C1

Plasmid

#202962

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC121

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC121

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Salsa-BC282-P3H6

Plasmid

#203123

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC282

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC282

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Salsa-BC232-P3D4

Plasmid

#203073

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC232

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC232

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Salsa-BC216-P3B12

Plasmid

#203057

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC216

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC216

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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Salsa-BC308-P4F3

Plasmid

#203149

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC308

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC308

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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Salsa-BC306-P4F1

Plasmid

#203147

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC306

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC306

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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Salsa-BC307-P4F2

Plasmid

#203148

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC307

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC307

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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