We narrowed to 39,881 results for: lat

1. SAM-DNMT3A+sgAAVS1_145

   Plasmid

   #213166

   Purpose

   Vector with sgAAVS1 for induction of global DNA methylation.

   Depositor

   Sefi Rosenbluh

   Article

   Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

   Insert

   sgAAVS1_145
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   E1Fa and U6

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. SAM-DNMT3A+sgAAVS1_118

   Plasmid

   #213167

   Purpose

   Vector with sgAAVS1 for induction of global DNA methylation.

   Depositor

   Sefi Rosenbluh

   Article

   Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

   Insert

   sgAAVS1_118
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   E1Fa and U6

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. SAM-DNMT3A-inactiveEmpty

   Plasmid

   #213168

   Purpose

   Empty Vector (no sgRNA) used as a negative control (inactive DNMT3A) for induction of global DNA methylation.

   Depositor

   Sefi Rosenbluh

   Article

   Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   E1Fa and U6

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXD70DAmt1-DadR/DadS-Pdadh-DadhABC(R506S, A2)

   Plasmid

   #191631

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain (R506S mutation) with transcriptional regulators DadR/DadS

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   Dopamine dehydroxylase (R506S mutation, inactive form)
   

   Expression

   Bacterial

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXD71Cas10.0RTcgr-Pct5.1-crRNA(AarI)-RT(Δcgr12)

   Plasmid

   #191651

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer) cgr1/cgr2-deleting repair template, used as negative control for pXD71Cas10.4RT4

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   CymR
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTS2308-Tier1-pGL3-PPGK-NLuc_TB

   Plasmid

   #169551

   Purpose

   Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   pGL3-insulator-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
   

   Expression

   Mammalian

   Promoter

   PPGK

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTER SADB shRNA Human

   Plasmid

   #67151

   Purpose

   Doxycycline-regulated mammalian expression vector for expressing shRNA against human SADB

   Depositor

   Ana Carrera, Maria Alvarado-Kristensson

   Article

   Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

   Insert

   BRSK1 (BRSK1 Human)
   

   Use

   RNAi

   Expression

   Mammalian

   Promoter

   H1 Tet

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pET28aAviHisCD72aCTLD

   Plasmid

   #129065

   Purpose

   generation of biotinylated mouse CD72a CTLD protein using bacteria

   Depositor

   Takeshi Tsubata

   Article

   Akatsu et al J Exp Med. 2016 Nov 14;213(12):2691-2706

   Insert

   CD72a (Cd72 Mouse)
   

   Expression

   Bacterial

   Availability

   Academic Institutions and Nonprofits only

   

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9. pShuttle-EGFP-CLASP2 340-1362 9xS/A

   Plasmid

   #24373

   Depositor

   Torsten Wittmann

   Article

   Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

   Insert

   CLASP2 (340-1362) 9xS/A (CLASP2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Nonphosphorylatable CLASP2 deletion mutant. M…

   Available Since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-ZIP(WT)

    Plasmid

    #27134

    Depositor

    Ron Vale

    Article

    Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

    Insert

    CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Mouse, Human)
    

    Use

    Lentiviral

    Tags

    eGFP and mCherry

    Expression

    Mammalian

    Available Since

    April 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLQ4428 pHR (EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE)

    Plasmid

    #214885

    Purpose

    Lentiviral vector encoding TMP-regulatable RfxCas13d-DD fusion

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-FLAG-Akt2-CA

    Plasmid

    #64050

    Purpose

    Myristoylated-FLAG-tagged Akt2, constitutively active

    Depositor

    Weiping Han

    Article

    Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

    Insert

    AKT2 (AKT2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and MYR

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUT_mNF-Cas13d-Triplex

    Plasmid

    #227709

    Purpose

    LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Include a 3' UTR Triplex motif as a template backbone for MONARCH 2.0 system.

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    mCherry-P2A-RfxCas13d-Triplex
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV-d2i

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHub10-His-YFP-PIP2;1

    Plasmid

    #99386

    Purpose

    overexpression and isolation of recombinant At-PIP2;1 protein

    Depositor

    Ning Li

    Article

    Qing et al Mol Plant. 2016 Jan 4;9(1):158-74. doi:

    Insert

    PIP2;1 (PIP2A Mustard Weed)
    

    Tags

    His and YFP

    Expression

    Plant

    Promoter

    2xCaMV35S

    Available Since

    Aug. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-mCherry-TSC2(AA,3Q)

    Plasmid

    #192444

    Purpose

    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q ) (TSC2 Human)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-CLCb (EED/QQN)

    Plasmid

    #47422

    Depositor

    Peter McPherson

    Article

    Poupon et al Proc Natl Acad Sci U S A. 2008 Jan 8;105

    Insert

    CLCb EED/QQN (CLTB Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EED changed to QQN, in aa20-41 region

    Promoter

    CMV

    Available Since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pGEX-2T-TRR-C421-Y2404F

    Plasmid

    #182846

    Purpose

    mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG induction

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2014 Mar;141(5):1129-39. do

    Insert

    TRR 2011-2431 (trr Fly)
    

    Expression

    Bacterial

    Mutation

    mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 20…

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAdEasy-EGFP-CLASP2 340-1084 9xS/A

    Plasmid

    #24525

    Depositor

    Torsten Wittmann

    Article

    Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

    Insert

    CLASP2 (340-1084) 9xS/A (CLASP2 Human)
    

    Use

    Adenoviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Nonphosphorylatable CLASP2 deletion mutant. M…

    Available Since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pShuttle-EGFP-CLASP2 340-1084 9xS/A

    Plasmid

    #24384

    Depositor

    Torsten Wittmann

    Article

    Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

    Insert

    CLASP2 (340-1084) 9xS/A (CLASP2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Nonphosphorylatable CLASP2 deletion mutant. M…

    Available Since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SAM-DNMT3A-inactive+sgGFP

    Plasmid

    #213169

    Purpose

    Vector with sgGFP used as a negative control (inactive DNMT3A) for induction of global DNA methylation.

    Depositor

    Sefi Rosenbluh

    Article

    Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

    Insert

    sgGFP
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    E1Fa and U6

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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