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pDONR221_zCas9

Plasmid

#213913

Purpose

Entry clone containing 3×FLAG-NLS-zCas9-NLS encoding sequence, Pisum sativum rbcS E9 terminator. For use in plants and compatible with the MultiSite Gateway system

Depositor

Sébastien Mongrand

Article

Li et al Bio Protoc. 2024 Jul 5;14(13):e5029. doi

Insert

zCas9

Use

CRISPR

Mutation

Zea mays codon-optimized Cas9

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pJET-TtrpC

Plasmid

#213467

Purpose

Vector containing the TrpC terminator for Golden Gate cloning in the Fg vector

Depositor

Kim Hammond-Kosack

Article

Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

Insert

Terminator of tryptophan synthase of Aspergillus nidulans

Expression

Bacterial

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJET-PtrpC

Plasmid

#213465

Purpose

Vector containing the TrpC promoter for Golden Gate cloning in the Fg vector

Depositor

Kim Hammond-Kosack

Article

Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

Insert

Promoter of tryptophan synthase of Aspergillus nidulans

Expression

Bacterial

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pDESTpK7WG2-RPS5Ap-NLS-OleGFP

Plasmid

#193370

Purpose

nTALEN or nTALECD vector assembly, Destination vector for plants to express ORFs from entry clone with NLS under control of the RPS5A promoter and HSP terminator.

Depositor

Shin-ichi Arimura

Article

Hosoda et al Plant Biotechnol (Tokyo). 2024 Dec 25;41

Type

Empty backbone

Expression

Plant

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E3_pENTR_L3-L2_HD_G1397-DddtoxA-N

Plasmid

#191596

Purpose

ptpTALECD vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E3_pENTR_L3-L2_NI_G1397-DddtoxA-N

Plasmid

#191597

Purpose

ptpTALECD vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E3_pENTR_L3-L2_NN_G1397-DddtoxA-N

Plasmid

#191598

Purpose

ptpTALECD vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E1_pENTR_L1-L4_HD_G1397-DddtoxA-C

Plasmid

#191583

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E1_pENTR_L1-L4_NG_G1397-DddtoxA-C

Plasmid

#191584

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E1_pENTR_L1-L4_HD_G1397-DddtoxA-N

Plasmid

#191585

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor

Use

Other

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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