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We narrowed to 9,922 results for: pET

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Showing: 9821 - 9840 of 9922 results

  2. N229A Lo-MetQ

    Plasmid
    #118268
    Purpose
    N229A Mutation in Methionine Binding Protein (MetQ) used to solve crystal structure
    Depositor
    Douglas Rees
    Publication
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Insert
    MetQ (N229A) (metQ E. coli)
    Tags
    10x His Tag and Enterokinase Cleavage Site
    Expression
    Bacterial
    Mutation
    Changed Asparagine (Asn) 229 to Alanine (Ala)
    Promoter
    T7 Promoter
    Available since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    118268-plasmid-map-sequence-id-228534

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  9. PssaB-sfGFP-mRuby2

    Plasmid
    #257838
    Purpose
    Transcriptional reporter for SPI-2 (PssaB-sfGFP - no degradation tag) with constitutive mRuby2
    Depositor
    Keara Lane
    Publication
    Spratt et al Current Biology, Volume 36, Issue 13, 20
    Inserts
    PssaB-BBa_B0034-sfGFP-L3S2P21
    SJM901-PET3a_RBS-mRuby2-Bba_B0015
    Use
    Golden gate (ecoflex)
    Expression
    Bacterial
    Promoter
    PssaB and SJM901
    Available since
    Aug. 18, 2026
    Availability
    Academic Institutions and Nonprofits only
    257838-plasmid-map-sequence-id-515746

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  10. PssaG-sfGFP-mRuby2

    Plasmid
    #257840
    Purpose
    Transcriptional reporter for SPI-2 (PssaG-sfGFP - no degradation tag) with constitutive mRuby2
    Depositor
    Keara Lane
    Publication
    Spratt et al Current Biology, Volume 36, Issue 13, 20
    Inserts
    PssaG-BBa_B0034-sfGFP-L3S2P21
    SJM901-PET3a_RBS-mRuby2-Bba_B0015
    Use
    Golden gate (ecoflex)
    Expression
    Bacterial
    Promoter
    SJM901 and ssaG
    Available since
    Aug. 18, 2026
    Availability
    Academic Institutions and Nonprofits only
    257840-plasmid-map-sequence-id-515747

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  13. Ce iPGM-C-HiBiT

    Plasmid
    #207120
    Purpose
    Bacterially expressed iPGM-HiBiT for Ni-NTA purification
    Depositor
    James Inglese
    Publication
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    C. elegans-iPGM HiBIT His10 (ipgm-1 Nematode)
    Tags
    HiBiT Tag and Thrombin cleavage site + 10x His Ta…
    Expression
    Bacterial
    Mutation
    Codon optmization for expression in BL21DE3
    Promoter
    T7 Promotor
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    207120-plasmid-map-sequence-id-417169

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  14. T7 Lig-N-HiBiT

    Plasmid
    #215479
    Purpose
    Bacterially expressed T7 Lig-N-HiBiT for Ni-NTA purification
    Depositor
    James Inglese
    Publication
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Escherichia phage T7 DNA Ligase, N-terminal HiBiT, N-terminal 10XHis.Thrombin tag
    Tags
    10xHis-tag with thrombin site and HiBiT tag
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression, T7 gene…
    Promoter
    T7
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215479-plasmid-map-sequence-id-428721

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  16. *MtbCM-bio-His

    Plasmid
    #186027
    Purpose
    Expresses secreted Mycobacterium tuberculosis (M. tb) chorismate mutase (CM) containing a C-terminal biotinylation site and His tag
    Depositor
    James Inglese
    Publication
    van Neer et al ACS Infect Dis. 2025 Mar 14;11(3):703-71
    Insert
    Rv1885c Chorismate Mutase
    Tags
    Avi-tag for in vitro biotinylation and His-tag wi…
    Expression
    Bacterial
    Mutation
    Codon optimization for expression in BL21DE3
    Promoter
    T7 Promotor
    Available since
    March 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    186027-plasmid-map-sequence-id-370660

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  18. pJB55

    Plasmid
    #226310
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Publication
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    CsoS2 with V(T/S)G to AAA CsoS2 MR1-5
    Expression
    Bacterial
    Mutation
    VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…
    Promoter
    pTRC
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226310-plasmid-map-sequence-id-449922

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Showing: 9821 - 9840 of 9922 results