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We narrowed to 9,832 results for: pET

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Showing: 9821 - 9832 of 9832 results

  1. His6-SUMO-SPMc-SpyCatcher003-Ctag

    Plasmid
    #246648
    Purpose
    Expresses His6-SUMO-SPMc-SpyCatcher003-Ctag in bacterial cells for calcium-induced NeissLock protein ligation
    Depositor
    Mark Howarth
    Article
    Lim et al ACS Chem Biol. 2025 Sep 15. doi: 10.1021
    Insert
    His6-SUMO-SPMc-SpyCatcher003-Ctag
    Tags
    Ctag, His-tag, SUMO, and SpyCatcher003
    Expression
    Bacterial
    Mutation
    The SPMc comprises the C-terminal fragment of th…
    Promoter
    T7
    Available Since
    Oct. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    246648-plasmid-map-sequence-id-491284

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  2. cAMP-dependent protein kinase (PKA) regulatory 1a subunit (R1a)

    Plasmid
    #215436
    Purpose
    Bacterially expressed R1a (no HiBiT) Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Human cAMP-dependent protein kinase type I-alpha subunit (PRKAR1A) mRNA, complete cds, C-terminal 10X-His.Thrombin Tag
    Tags
    10X His-tag with thrombin site
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression, T7 gene…
    Promoter
    T7 Promotor
    Available Since
    June 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    215436-plasmid-map-sequence-id-428696

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  3. HisGB1-Fusion + CBP TAZ1(340-439)

    Plasmid
    #173762
    Purpose
    Bacterial coexpression of CBP TAZ1 and CITED2/Hif1a activation domain constructs
    Depositor
    Peter Wright
    Article
    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(
    Inserts
    CITED2(216-246):Hif1a(796-826) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    Tags
    His6GB1 and thrombin
    Expression
    Bacterial
    Mutation
    fusion of CITED2 and Hif1a
    Promoter
    T7
    Available Since
    Feb. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    173762-plasmid-map-sequence-id-353460

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  4. HisGB1-FusionL21A + CBP TAZ1(340-439)

    Plasmid
    #173763
    Purpose
    Bacterial Coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructs
    Depositor
    Peter Wright
    Article
    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(
    Inserts
    CITED2(216-246L231A):Hif1a(796-826) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    Tags
    His6GB1 and thrombin
    Expression
    Bacterial
    Mutation
    fusion of CITED2 and Hif1a
    Promoter
    T7
    Available Since
    Feb. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    173763-plasmid-map-sequence-id-353459

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  5. HIsGB1-FusionL63A + CBP TAZ1(340-439)

    Plasmid
    #173764
    Purpose
    Bacterial coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructs
    Depositor
    Peter Wright
    Article
    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(
    Inserts
    CITED2(216-246):Hif1a(796-826L822A) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    Tags
    His6GB1 and thrombin
    Expression
    Bacterial
    Mutation
    fusion of CITED2 and Hif1a
    Promoter
    T7
    Available Since
    Feb. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    173764-plasmid-map-sequence-id-353458

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  6. ybbR-ddfln4-RLC-ELP-LA-Xmod-DocIII-Ctag

    Plasmid
    #257336
    Purpose
    Polyprotein for applying force to the N-terminus of the RLC and the C-terminus of the lever arm of the RLC complex of human β-cardiac myosin
    Depositor
    Tom Perkins
    Article
    Deopa et al ACS Nano. 2026 Jun 9;20(22):15982-16000.
    Inserts
    RLC (MYL2 Human)
    Lever Arm (MYH7 Human)
    Tags
    Ctag, Dockerin III, ELP120, TEV, Xmod, ddfln4, an…
    Expression
    Bacterial
    Mutation
    only amino acids # 810-846 are used
    Promoter
    T7
    Available Since
    July 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    257336-plasmid-map-sequence-id-512392

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  7. AviTag-His6-OAZ-SPM(FrpA)-Ctag

    Plasmid
    #246646
    Purpose
    Expresses AviTag-His6-OAZ-SPM(FrpA)-Ctag in bacterial cells for calcium-induced NeissLock protein ligation
    Depositor
    Mark Howarth
    Article
    Lim et al ACS Chem Biol. 2025 Sep 15. doi: 10.1021
    Insert
    AviTag-His6-OAZ-SPM(FrpA)-Ctag
    Tags
    AviTag, Ctag, and His-tag
    Expression
    Bacterial
    Mutation
    Residues 95-219 of human Orinithine Decarboxylase…
    Promoter
    T7
    Available Since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    246646-plasmid-map-sequence-id-499057

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  8. RLC-ELP-ybbR-LA-GB1-GB1-Xmod-DocIII-Ctag

    Plasmid
    #257334
    Purpose
    Polyprotein for applying force to the N- and C-termini of the lever arm in the RLC complex of human β-cardiac myosin
    Depositor
    Tom Perkins
    Article
    Deopa et al ACS Nano. 2026 Jun 9;20(22):15982-16000.
    Inserts
    RLC (MYL2 Human)
    Lever Arm (MYH7 Human)
    Tags
    Ctag, Dockerin III, ELP120 (Elastin-Like Polypept…
    Expression
    Bacterial
    Mutation
    only amino acids # 810-846 are used
    Promoter
    T7
    Available Since
    July 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    257334-plasmid-map-sequence-id-512397

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  9. LA-ELP-ybbR-RLC-ddfln4-Xmod-DocIII-Ctag

    Plasmid
    #257335
    Purpose
    Polyprotein for applying force to the N- and C-termini of the RLC in the RLC complex of human β-cardiac myosin
    Depositor
    Tom Perkins
    Article
    Deopa et al ACS Nano. 2026 Jun 9;20(22):15982-16000.
    Inserts
    RLC (MYL2 Human)
    Lever Arm (MYH7 Human)
    Tags
    Ctag, Dockerin III, ELP120 (Elastin-Like Polypept…
    Expression
    Mammalian
    Mutation
    only amino acids # 810-846 are used
    Promoter
    T7
    Available Since
    July 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    257335-plasmid-map-sequence-id-515911

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  10. ΔN_RLC-ELP-ybbR-LA-GB1-GB1-Xmod-DocIII-Ctag

    Plasmid
    #257337
    Purpose
    Polyprotein for applying force to the N- and C-termini of the lever arm in the RLC complex of human β-cardiac myosin, where residues of 2-22 of the RLC were deleted.
    Depositor
    Tom Perkins
    Article
    Deopa et al ACS Nano. 2026 Jun 9;20(22):15982-16000.
    Inserts
    RLC (MYL2 Human)
    Lever Arm (MYH7 Human)
    Tags
    Ctag, Dockerin III, ELP120, GB1, TEV (TEV cleavag…
    Expression
    Bacterial
    Mutation
    Amino acids 2 to 22 are deleted and only amino ac…
    Promoter
    T7
    Available Since
    July 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    257337-plasmid-map-sequence-id-512398

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  11. pABDP2

    Plasmid
    #249501
    Purpose
    Bidirectional chloroplast promoter (BDP2) enabling co-expression for transformation of Chlamydomonas reinhardtii photosynthetically deficient strain CC4388
    Depositor
    Isabel Desgagné-Penix
    Inserts
    mVenus (yellow/green fluorescence)
    tdTomato (red fluorescence)
    Use
    Synthetic Biology; Chlamydomonas reinhardtii chlo…
    Expression
    Bacterial
    Mutation
    Change of F46L, F64L, M153T, V163A, S175G and td…
    Promoter
    Bidirectional chloroplast promoter enabling co-ex…
    Available Since
    Jan. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    249501-plasmid-map-sequence-id-497979

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  12. AviTag-His6-OAZ-SPM(FrpA 298-318)-GSG-SpyTag003

    Plasmid
    #246647
    Purpose
    Expresses AviTag-His6-OAZ-SPM(FrpA 298-318)-GSG-SpyTag003 in bacterial cells for calcium-induced NeissLock protein ligation
    Depositor
    Mark Howarth
    Article
    Lim et al ACS Chem Biol. 2025 Sep 15. doi: 10.1021
    Insert
    AviTag-His6-OAZ-SPM(FrpA 298-318)-GSG-SpyTag003
    Tags
    AviTag, His-tag, and SpyTag003
    Expression
    Bacterial
    Mutation
    Orinithine Decarboxylase Antizyme (OAZ) with C175…
    Promoter
    T7
    Available Since
    Oct. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    246647-plasmid-map-sequence-id-491279

    Enlarge

Showing: 9821 - 9832 of 9832 results