We narrowed to 11,805 results for: nsf

1. qTAG-C-Solo-mStayGold-CANX

   Plasmid

   #227280

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the CANX locus. For ER visualization. To be co-transfected with sgRNA plasmid px330-CANX (Addgene #227279)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   CANX Homology Arms flanking a mStayGold Tag (CANX Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-TRE-DIO-ChR2-EYFP

   Plasmid

   #183765

   Purpose

   This plasmid is for use with neuronal cell-type selective activity tagging. The ChR2-EYFP expression requires both neural activity and Cre recombinase.

   Depositor

   William Wisden

   Article

   Yu et al Science. 2022 Jul;377(6601):63-72. doi:

   Insert

   AAV transgene -teto promoter-flex/dio-ChR2-EYFP
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Tags

   ChR2-EYFP

   Expression

   Mammalian

   Promoter

   tetO promoter

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-miniDq-P2A-mCherry

   Plasmid

   #204357

   Purpose

   AAV vector for miniDq expression under the control of human synapsin promoter

   Depositor

   Masao Doi

   Article

   Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

   Insert

   miniDq-P2A-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Mutation

   The third intracellular loop (ICL3) of hM3Dq was …

   Promoter

   hSyn

   Available Since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZac2.1 GfaABC1D hM3D mCherry SV40

   Plasmid

   #92284

   Purpose

   AAV DREADD expression

   Depositor

   Baljit Khakh

   Article

   Chai et al Neuron. 2017 Aug 2;95(3):531-549.e9. doi

   Insert

   hM3D (CHRM3 Human)
   

   Use

   AAV

   Tags

   mCherry

   Promoter

   GfaABC1D

   Available Since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-FLEX-SaCas9-U6-sgGria1

   Plasmid

   #124853

   Purpose

   Mutagenesis of Gria1

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gria1 (Gria1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EF1a-mCherry-IRES-Flpo

   Plasmid

   #55634

   Purpose

   Expresses Flpo in Mammalian Cells

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde and AAV1

   Inserts

   mCherry
   Flpo
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Ef1a and Ef1a/IRES

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-C-Solo-mStayGold-VIM

   Plasmid

   #227302

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the VIM locus. For intermediate filament visualization. To be co-transfected with sgRNA plasmid px330-PITCh-VIM (Addgene #227301)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   VIM Homology Arms flanking a mStayGold Tag (VIM Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-FLEX-ArchT-tdTomato

   Plasmid

   #28305

   Purpose

   Cre-dependent viral expression of ArchT-tdTomato for neuronal inhibition

   Depositor

   Edward Boyden

   Article

   Han et al Front Syst Neurosci. 2011;5:18. Epub 201

   Has Service

   AAV5

   Insert

   ArchT-tdTomato
   

   Use

   AAV and Cre/Lox; Adeno-associated virus

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CAG

   Available Since

   Aug. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-UBC-S2-cAMPr

   Plasmid

   #160723

   Purpose

   Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter cAMPr (cAMP indicator), HA tag, and S2 protein scaffold.

   Depositor

   Edward Boyden

   Article

   Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

   Insert

   S2-cAMPr
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Human ubiquitin C (UBC) promoter

   Available Since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1_-DIO-GRAB_NPY1.0

    Plasmid

    #208677

    Purpose

    Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0
    

    Use

    AAV

    Promoter

    EF1_

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn1-DIO-ExRai-AKAR2

    Plasmid

    #171846

    Purpose

    Cre-dependent, synapsin promoter-driven expression of the green fluorescent ExRai-AKAR2 PKA biosensor in neurons.

    Depositor

    Richard Huganir

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    ExRai-AKAR2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn1

    Available Since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV CD68-hM4D(Gi)-mCherry

    Plasmid

    #75033

    Purpose

    Expression of hM4D(Gi) using the CD68 promoter.

    Depositor

    Bryan Roth

    Article

    Grace et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Has Service

    AAV1 and AAV9

    Insert

    hM4D(Gi)-mCherry
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CD68

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. qTAG-C-Solo-mStayGold-TOMM20

    Plasmid

    #227307

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TOMM20 Homology Arms flanking a mStayGold Tag (TOMM20 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn Con/Fon EYFP

    Plasmid

    #55650

    Purpose

    Cre-on/Flp-on EYFP under the Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde and AAV8

    Insert

    eYFP with introns
    

    Use

    AAV; Cre on/flp on eyfp

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. hSyn-somBiPOLES-mCerulean

    Plasmid

    #154945

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Promoter

    human synapsin

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-DIO-GRAB_ACh3.0

    Plasmid

    #121923

    Purpose

    express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

    Insert

    GPCR activation based ACh sensor GRAB-ACh3.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. GLuc-PM

    Plasmid

    #164783

    Purpose

    Gaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors

    Depositor

    Laszlo Hunyady

    Article

    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.

    Insert

    Gaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Gaussia princeps, Human)
    

    Tags

    GLuc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-RHO-IntC-dCas9C-VPR-polyA

    Plasmid

    #166692

    Purpose

    Express C-terminal part of split dCas9-VPR

    Depositor

    Stylianos Michalakis

    Article

    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

    Insert

    IntC-dCas9C-VPR
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    short human rhodopsin promoter

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Ef1a-DIO hChR2(C128S/D156A)-EYFP

    Plasmid

    #35503

    Purpose

    AAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.

    Depositor

    Karl Deisseroth

    Article

    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

    Insert

    hChR2(C128S/D156A)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    C128S and D156A

    Promoter

    Ef1a

    Available Since

    April 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEMS2115

    Plasmid

    #49140

    Purpose

    The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    ssAAV-Ple155-emGFP-WPRE
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    MiniPromoter Ple155 from the human PCP2 gene

    Available Since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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