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pRK5R 15xGly-uFMR1-3xHA

Plasmid

#242191

Purpose

Transient expression of 15xGly-uFMR1-3xHA

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

15xGly-uFMR1-3xHA (FMR1 Human)

Tags

3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLJY1 DD-FAM98B[1-330]

Plasmid

#211353

Purpose

Constitutive lentiviral expression of DD-V5-FAM98B[1-330] fusion protein

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

DD-FAM98B[1-330] (FAM98B Human)

Use

Lentiviral

Tags

FKBP12 DD and V5

Mutation

Truncation of C-terminal glycine-rich IDR

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pRhobin2-H2B-mNG3K-IRES-NLS-mTagBFP-2xNLS

Plasmid

#239020

Purpose

Co-overexpression of Rhobin2-H2B-mNeonGreen3K fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

Depositor

William DeGrado, Bo Huang

Article

Chen et al bioRxiv 2025.06.24.661379

Insert

Rhobin2-H2B (H2BC21 Synthetic, Human)

Tags

TagBFP and mNeonGreen3K

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pRhobin9-H2B-mNG3K-IRES-NLS-mTagBFP-2xNLS

Plasmid

#239021

Purpose

Co-overexpression of Rhobin9-H2B-mNeonGreen3K fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

Depositor

William DeGrado, Bo Huang

Article

Chen et al bioRxiv 2025.06.24.661379

Insert

Rhobin9-H2B (H2BC21 Synthetic, Human)

Tags

TagBFP and mNeonGreen3K

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-TS.FF4x1-mRuby2-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235316

Purpose

Lentiviral expression of mRuby2 with 5' microRNA target site only (closed-loop)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Step-FLAG FBXO21

Plasmid

#236458

Purpose

transient overexpression of FBXO21 in mammalian cells

Depositor

Michele Pagano

Article

Donato et al Nat Cell Biol. 2017 Apr;19(4):341-351. d

Insert

FBXO21 (FBXO21 Human)

Tags

Strep-FLAG

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-TS.FF6x1-mRuby2-miRE.FF4-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235323

Purpose

Lentiviral expression of ComMAND open-loop circuit regulating mRuby2, design 3

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-TS.FF6x1-mRuby2-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235315

Purpose

Lentiviral expression of mRuby2 with 5' microRNA target site only (open-loop)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-TS.FF6x1-mRuby2(miRE.FF4)-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235321

Purpose

Lentiviral expression of ComMAND open-loop circuit regulating mRuby2, design 2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-mRuby2-TS.FF6x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235313

Purpose

Lentiviral expression of mRuby2 with 3' microRNA target site only (open-loop)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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