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pJethlpAmNeonGreenKm

Plasmid

#206816

Purpose

Template for hlpA-mneongreen amplification associated with kanamycin resistance gene

Depositor

Virginie Libante

Article

Mignolet et al bioRxiv 2025.05.10.653270

Insert

hlpA

Use

Synthetic Biology

Tags

mNeonGreen

Expression

Mutation

Promoter

Available since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAM76

Plasmid

#227625

Purpose

pET28a Ubiquitin-G77C78

Depositor

Andreas Martin

Article

Worden et al Mol Cell. 2017 Sep 7;67(5):799-811.e8. d

Insert

Ub (UBI4 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

G77C78

Promoter

T7

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM59

Plasmid

#227655

Purpose

pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, Δ78–81)

Depositor

Andreas Martin

Article

Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-

Inserts

Rpn11 (RPN11 Budding Yeast)
Rpn8 (RPN8 Budding Yeast)

Use

Tags

H6-PreScission and PreScission-Strep

Expression

Bacterial

Mutation

1-179 and amino acids 2-239, Δ78–81

Promoter

T7

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM57

Plasmid

#227654

Purpose

pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, V80A)

Depositor

Andreas Martin

Article

Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-

Inserts

Rpn11 (RPN11 Budding Yeast)
Rpn8 (RPN8 Budding Yeast)

Use

Tags

H6-PreScission and PreScission-Strep

Expression

Bacterial

Mutation

1-179 and amino acids 2-239, V80A

Promoter

T7

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM54

Plasmid

#227653

Purpose

pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, L132A)

Depositor

Andreas Martin

Article

Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-

Inserts

Rpn11 (RPN11 Budding Yeast)
Rpn8 (RPN8 Budding Yeast)

Use

Tags

H6-PreScission and PreScission-Strep

Expression

Bacterial

Mutation

1-179 and amino acids 2-239, L132A

Promoter

T7

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM198

Plasmid

#227657

Purpose

pRS305 His-HRV3C-Rpn5 WT

Depositor

Andreas Martin

Article

Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

Insert

rpn5 (RPN5 Budding Yeast)

Use

Tags

His-HRV3C-

Expression

Yeast

Mutation

Promoter

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM200

Plasmid

#227659

Purpose

pRS305 3XFLAG-HRV3C-Rpn5 WT

Depositor

Andreas Martin

Article

Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

Insert

rpn5 (RPN5 Budding Yeast)

Use

Tags

3X FLAG-HRV3C-

Expression

Yeast

Mutation

Promoter

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM201

Plasmid

#227660

Purpose

pRS305 3XFLAG-HRV3C-Rpn5-VTENKIF

Depositor

Andreas Martin

Article

Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

Insert

rpn5 (RPN5 Budding Yeast)

Use

Tags

3x-FLAG-HRV3C-

Expression

Yeast

Mutation

(amino acids 121-131) VTENKIF mutated to AAAAAAA

Promoter

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM202

Plasmid

#227661

Purpose

pRS316 Rpn6 promoter-Rpn5 ORF-Rpn5 terminator

Depositor

Andreas Martin

Article

Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

Insert

rpn5 (RPN5 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM72

Plasmid

#227622

Purpose

pETDuet-1 Rpn8(1-179)-precission-Strep, H6-precission-Rpn11(2-239, G77P)

Depositor

Andreas Martin

Article

Worden et al Mol Cell. 2017 Sep 7;67(5):799-811.e8. d

Inserts

Rpn11 (RPN11 Budding Yeast)
rpn8 (RPN8 Budding Yeast)

Use

Tags

His-Prescission

Expression

Bacterial

Mutation

1-179 and aa 2-239, G77P

Promoter

T7

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM71

Plasmid

#227621

Purpose

pETDuet-1 H6-precission-Rpn11(24-160-GGSGSG-192-221) , Rpn8(1-179)

Depositor

Andreas Martin

Article

Worden et al Mol Cell. 2017 Sep 7;67(5):799-811.e8. d

Inserts

Rpn11 (RPN11 Budding Yeast)
rpn8 (RPN8 Budding Yeast)

Use

Tags

His-Prescission

Expression

Bacterial

Mutation

1-179 and amino acids 24-160-GGSGSG-192-221

Promoter

T7

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM248

Plasmid

#226892

Purpose

6xHis-PSP-GS-PANN(75-150)-(5aaGS)-Msp1(36-362)_W166A/Y167A

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

msp1 (MSP1 Budding Yeast)

Use

Tags

His-Precission-PANN

Expression

Bacterial

Mutation

W166A/Y167A

Promoter

T7

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAM298

Plasmid

#224257

Purpose

MCM WT ubiquitin

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

MCM Ubiquitin

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pAM75

Plasmid

#224249

Purpose

pET28a MWG-ubiquitin-G77-C78

Depositor

Andreas Martin

Article

Xie et al Methods Mol Biol. 2023;2591:79-100. doi:

Insert

MWG-ubiquitin-G77-C78

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pAM299

Plasmid

#224258

Purpose

MCMUbHis

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

MC-Ub-3C-His

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pAM257

Plasmid

#226901

Purpose

6xHis-PSP-MBP-Pex3(40-441)

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

pex3 (PEX3 Budding Yeast)

Use

Tags

6xHis-PSP-MBP

Expression

Bacterial

Mutation

amino acids 40-441

Promoter

T7

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM254

Plasmid

#226898

Purpose

mEOS3.2-IDR*-(7aaSG)-PSP-GSS-FLAG-6xHis

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

mEOS3.2

Use

Tags

7aaSG Prescission GSS-FLAG-6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM259

Plasmid

#226903

Purpose

mEOS3.2-MBP-Pex15(1-253)-PSP-FLAG-6xHis

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

mEOS3.2

Use

Tags

mEOS3.2-MBP-Pex15(1-253)-PSP-FLAG-6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM253

Plasmid

#226897

Purpose

mEOS3.2-PSP-GSS-FLAG-6xHis

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

mEOS3.2

Use

Tags

Prescission GSS-FLAG-6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM256

Plasmid

#226900

Purpose

mEOS3.2-GS-SUMO-1(3-103)-Pex15(1-309)-PSP-GSS-FLAG-6xHis

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

mEOS3.2

Use

Tags

GS-SUMO-1(3-103)-Pex15(1-309)-PSP-GSS-FLAG-6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge