Addgene

Showing: 81 - 100 of 585 results

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Nano-lantern(Ca2+)_63/pcDNA3

Plasmid

#51986

Purpose

mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=63nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM_E104Q-4GS

Use

Tags

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Availability

Academic Institutions and Nonprofits only
Nano-lantern(Ca2+)_11/pcDNA3

Plasmid

#51987

Purpose

mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=11nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM-4GS

Use

Tags

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Availability

Academic Institutions and Nonprofits only
Anti-Cavbeta1 Ca2+ channel [N7/18.1R]

Plasmid

#114497

Purpose

Mammalian Expression Plasmid of anti-Cavbeta1 Ca2+ channel (Human). Derived from hybridoma N7/18.1.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Cavbeta1 Ca2+ channel (Home sapiens) recombinant mouse monoclonal antibody (CACNB1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Availability

Academic Institutions and Nonprofits only

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Anti-Cavbeta2 Ca2+ channel [N8B/1R]

Plasmid

#149458

Purpose

Mammalian Expression Plasmid of anti-Cavbeta2 Ca2+ channel (Rat). Derived from hybridoma N8B/1.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

Mammalian Expression of anti-Cavbeta2 Ca2+ channel (Rat) recombinant mouse monoclonal antibody (Cacnb2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Availability

Academic Institutions and Nonprofits only

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Cav1.2 Ca2+ channel scFv [N263/31]

Plasmid

#182083

Purpose

Mammalian Expression of Cav1.2 Ca2+ channel scFv. Derived from hybridoma N263/31.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Cav1.2 Ca2+ channel (Rattus norvegicus) (Cacna1c Mouse)

Use

Tags

HA, His

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Anti-Cavbeta4 Ca2+ channel [N10/7R]

Plasmid

#177492

Purpose

Mammalian Expression Plasmid of anti-Cavbeta4 Ca2+ channel (Rat). Derived from hybridoma N10/7.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Cavbeta4 Ca2+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Cacnb4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Cav1.3 Ca2+ channel [N38/8R]

Plasmid

#177540

Purpose

Mammalian Expression Plasmid of anti-Cav1.3 Ca2+ channel (Rat). Derived from hybridoma N38/8.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Cav1.3 Ca2+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Cacna1d Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Availability

Academic Institutions and Nonprofits only

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pCA23-sgRNA vector-U6-sgTS2 (SpCas9)

Plasmid

#199454

Purpose

U6-driven sgRNA targeting TS2 sequence (GGAGCTTACTGAGACTCTTC)

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

TS2 sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

mouse U6

Availability

Academic Institutions and Nonprofits only

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pCA27-pHR-MiniCMV-TriTag (mTagBFP)

Plasmid

#199458

Purpose

Expression of TriTag (mTagBFP) in mammalian cells

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

TriTag

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

MiniCMV

Availability

Academic Institutions and Nonprofits only

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pCA28-pMa-PspCas13b crRNA-TS1

Plasmid

#199459

Purpose

U6-driven crRNA targeting TS1 sequence (CCTCCTCGGAGAGCATCGGTGC )

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

TS1 crRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

mouse U6

Availability

Academic Institutions and Nonprofits only

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Anti-Cav3.1 Ca2+ channel [N178A/9R-2b]

Plasmid

#206660

Purpose

Mammalian Expression Plasmid of anti-Cav3.1 Ca2+ channel (Mouse). Derived from hybridoma N178A/9-2b.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Cav3.1 Ca2+ channel (Mus musculus) recombinant Mouse monoclonal antibody (Cacna1g Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Cavbeta1 Ca2+ channel [N7/18R-2b]

Plasmid

#206676

Purpose

Mammalian Expression Plasmid of anti-Cavbeta1 Ca2+ channel (Human). Derived from hybridoma N7/18-2b.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Cavbeta1 Ca2+ channel (Homo sapiens) recombinant Mouse monoclonal antibody (CACNB1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
Cav1.3 Ca2+ channel scFv [N473/36]

Plasmid

#206786

Purpose

Mammalian Expression of Cav1.3 Ca2+ channel scFV. Derived from hybridoma N473/36 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Cav1.3 Ca2+ channel (Homo sapiens) recombinant scFV (CACNB3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
Cav1.3 Ca2+ channel scFv [L48A/9]

Plasmid

#190515

Purpose

Mammalian Expression of Cav1.3 Ca2+ channel scFV. Derived from hybridoma L48A/9.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Cav1.3 Ca2+ channel (Rattus norvegicus) recombinant scFV (Cacna1d Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Carl-CeNL(Ca2+)_110μ-KDEL-pcDNA3

Plasmid

#111925

Purpose

Cyan color luminescent Ca2+ indicator targeted to endoplasmic reticulum. Calreticulin and a KDEL signal for ER retention located at the N-terminus and C-terminus of CeNL(Ca2+)_110μ

Depositor

Takeharu Nagai

Article

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

CeNL(Ca2+)_110μ

Use

Tags

Calreticulin and KDEL

Expression

Mammalian

Mutation

E31D, F92W, E104D. D133E at CaM

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Mito-Cyan Nano-lantern(Ca2+)/pcDNA3

Plasmid

#71345

Purpose

Express Mito-Cyan Nano-lantern-based luminescent Ca2+ indicator in mammalian cel

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

hOTC_Cyan Nano-lantern(Ca2+)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
Nuc-Orange Nano-lantern(Ca2+)/pcDNA3

Plasmid

#71349

Purpose

Express Nuc-Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cel

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Orange Nano-lantern(Ca2+)_H2B

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
Anti-Cav3.1 Ca2+ channel [N178A/9.1R]

Plasmid

#114520

Purpose

Mammalian Expression Plasmid of anti-Cav3.1 Ca2+ channel (Mouse). Derived from hybridoma N178A/9.1.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Cav3.1 Ca2+ channel (Mus musculus) recombinant mouse monoclonal antibody (Cacna1g Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Cav1.2 Ca2+ channel [L57/23R]

Plasmid

#177484

Purpose

Mammalian Expression Plasmid of anti-Cav1.2 Ca2+ channel (Rabbit). Derived from hybridoma L57/23.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Cav1.2 Ca2+ channel (Oryctolagus cuniculus) recombinant mouse monoclonal antibody (CACNA1C Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Cav1.2 Ca2+ channel [N263/31R]

Plasmid

#177523

Purpose

Mammalian Expression Plasmid of anti-Cav1.2 Ca2+ channel (Rat). Derived from hybridoma N263/31.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Cav1.2 Ca2+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Cacna1c Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Availability

Academic Institutions and Nonprofits only

Enlarge