We narrowed to 241 results for: hedgehog

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pCS2 YFP-Smo-Weyer's

Plasmid

#41014

Depositor

Rajat Rohatgi

Publication

Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

Insert

smoothened homolog (Smo Mouse)

Tags

EVC2 (aa 1177-1220) and YFP

Expression

Mammalian

Mutation

YFP inserted after Smo signal sequence. Transfer…

Available since

Nov. 16, 2012

Availability

Academic Institutions and Nonprofits only

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RCAS(A) cIhh (CT#432)

Plasmid

#13998

Depositor

Cliff Tabin

Publication

Vortkamp et al Science. 1996 Aug 2. 273(5275):613-22.

Insert

Ihh (IHH Chicken)

Use

Retroviral; Avian expression

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
GBSGFP insulator

Plasmid

#121975

Purpose

Gli activity reporter, mouse transgenesis

Depositor

James Briscoe

Publication

Balaskas et al Cell. 2012 Jan 20;148(1-2):273-84. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GBS(long)-GFP-insulator (Gnb5 Mouse)

Use

Mouse transgenesis

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only
pEF5/FRT/DEST-3xHA/mGli2

Plasmid

#51250

Purpose

Encodes N-3xHA-TEV-mouseGli2-C

Depositor

Rajat Rohatgi

Publication

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
DN2-Gli3 in AINGpCAGGS

Plasmid

#121969

Purpose

strong Gli3 transcriptional activator

Depositor

James Briscoe

Publication

Stamataki et al Genes Dev. 2005 Mar 1;19(5):626-41. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DN2Gli3 (GLI3 Human)

Use

Chick electroporation

Tags

5xmyc

Expression

Mammalian

Mutation

N terminus deletion (nt1-1401)

Available since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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PD23

Plasmid

#164536

Purpose

Expresses SmoM2-FLAG

Depositor

Gregory Pazour

Publication

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened W539L (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

W539L

Promoter

CMV

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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GP757

Plasmid

#164540

Purpose

Expresses Sstr3-FLAG

Depositor

Gregory Pazour

Publication

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Somatostatin receptor type 3 (Sstr3 Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

WT

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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GP736

Plasmid

#164532

Purpose

Express Smo-FLAG-Ub

Depositor

Gregory Pazour

Publication

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened (Smo Mouse)

Use

Lentiviral

Tags

FLAG-Ub

Mutation

WT

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGL2-ptc-lu

Plasmid

#37678

Depositor

Philip Beachy

Publication

Chen et al Cell. 1999 Aug 6;98(3):305-16.

Insert

ptc promoter (−758 to +130)

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

ptc

Available since

Nov. 12, 2012

Availability

Academic Institutions and Nonprofits only
DN1-Gli3 in AINGpCAGGS

Plasmid

#121970

Purpose

medium Gli3 transcriptional activator

Depositor

James Briscoe

Publication

Stamataki et al Genes Dev. 2005 Mar 1;19(5):626-41. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DN2Gli3 (GLI3 Human)

Use

Chick electroporation

Tags

5xmyc

Expression

Mammalian

Mutation

N terminus deletion (nt1-864)

Available since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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GP799

Plasmid

#164544

Purpose

Expresses Smo-FLAG

Depositor

Gregory Pazour

Publication

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

WT

Promoter

GgCryD1

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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GP735

Plasmid

#164537

Purpose

Expresses SmoM2-FLAG-Ub

Depositor

Gregory Pazour

Publication

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened W539L (Smo Mouse)

Use

Lentiviral

Tags

FLAG-Ub

Mutation

W539L

Promoter

CMV

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT-DEST EVC2dW-TEV-YFP-Flag

Plasmid

#41006

Depositor

Rajat Rohatgi

Publication

Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

Insert

Ellis van Creveld syndrome 2 (EVC2 Mouse)

Tags

TEV-YFP-Flag

Expression

Mammalian

Mutation

deleted amino acids 1177-1220

Available since

Nov. 15, 2012

Availability

Academic Institutions and Nonprofits only
pEF5/FRT-DEST EVC2dW-Flag

Plasmid

#41007

Depositor

Rajat Rohatgi

Publication

Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

Insert

Ellis van Creveld syndrome 2 (EVC2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 1177-1220

Available since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only
35 pCR vegf 121

Plasmid

#13060

Depositor

Nathan Lawson

Publication

Lawson et al Dev Cell. 2002 Jul . 3(1):127-36.

Insert

vegf121

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pGEX mPtch-Cterm

Plasmid

#58704

Purpose

used for anti-mouse Patched antibody production (for injection into animals)

Depositor

Matthew Scott

Publication

Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

Insert

Ptch (Ptch1 Mouse)

Tags

GST

Expression

Bacterial

Mutation

contains only Cterminus (aa ~1238-1413)

Promoter

tac

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only
pMal mPtch-Cterm

Plasmid

#58705

Purpose

used for anti-mouse Patched antibody production (for affinity purification)

Depositor

Matthew Scott

Publication

Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

Insert

Ptch (Ptch1 Mouse)

Tags

MBP

Expression

Bacterial

Mutation

contains only Cterminus (aa ~1205-1434)

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pMuLE_ENTR_CBF-GLuc_L3-L2

Plasmid

#113868

Purpose

Single pathway reporter; notch promoter-driven expression of secreted Gaussia luciferase; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Publication

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

CBF (notch dependent promoter)
GLuc

Use

Gateway Cloning

Expression

Mammalian

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pMuLE_ENTR_TOP-NLuc1.1_L5-L4

Plasmid

#113867

Purpose

Single pathway reporter; wnt promoter-driven expression of intracellular NanoLuc; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Publication

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

TOP (wnt dependent promoter)
NLuc

Use

Gateway Cloning

Expression

Mammalian

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A

Plasmid

#51248

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to Ala

Depositor

Rajat Rohatgi

Publication

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Mutation

amino acids 849,865,877,907,980,1006 mutated to A…

Promoter

EF1a

Available since

March 7, 2014

Availability

Academic Institutions and Nonprofits only