Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 137 results for: pyes

Showing: 81 - 100 of 137 results

  3. pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Monkey

    Plasmid
    #238132
    Purpose
    This plasmid encodes the full-length African Green Monkey (Chlorocebus sabaeus) TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.
    Depositor
    Dirk Daelemans
    Article
    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.
    Insert
    TMEM106B
    Use
    Lentiviral
    Tags
    Blasticidin-P2A-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    238132-plasmid-map-sequence-id-476568

    Enlarge

  4. pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Human

    Plasmid
    #238130
    Purpose
    This plasmid encodes the full-length human TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.
    Depositor
    Dirk Daelemans
    Article
    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.
    Insert
    TMEM106B (TMEM106B Human)
    Use
    Lentiviral
    Tags
    Blasticidin-P2A-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    238130-plasmid-map-sequence-id-476571

    Enlarge

  5. pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Mouse

    Plasmid
    #238133
    Purpose
    This plasmid encodes the full-length Mouse (Mus musculus) TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.
    Depositor
    Dirk Daelemans
    Article
    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.
    Insert
    TMEM106B (Tmem106b Mouse)
    Use
    Lentiviral
    Tags
    Blasticidin-P2A-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    238133-plasmid-map-sequence-id-476563

    Enlarge

  6. pLCKO-CMV-TMEM106B-M210A_F213A-P2A-Blasticidin

    Plasmid
    #237479
    Purpose
    Encodes full-length human TMEM106B. M210A/F213A mutations block S1 binding and SARS-CoV-2 Belgium/GHB-03021/2020 TMEM106B mediated infection, without affecting TMEM106B expression or localization.
    Depositor
    Dirk Daelemans
    Article
    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.
    Insert
    TMEM106B (TMEM106B Human)
    Use
    Lentiviral
    Tags
    P2A-Blasticidin
    Expression
    Mammalian
    Mutation
    M210A + F213A
    Promoter
    CMV
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    237479-plasmid-map-sequence-id-476565

    Enlarge

  7. pLCKO-CMV-SARS-CoV-2-S-Bel-P5_5-7-IRES-mNeonGreen-NES/PKI-P2A-Blasticidin

    Plasmid
    #237480
    Purpose
    Encodes full-length SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate), featuring the E484D substitution, which likely emerged during passaging and enhances TMEM106B binding.
    Depositor
    Dirk Daelemans
    Article
    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.
    Insert
    SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate)
    Use
    Lentiviral
    Tags
    IRES-mNeonGreen-NES/PKI and P2A-Blasticidin
    Expression
    Mammalian
    Mutation
    S484D + S813I + deleted amino acids 68-76 + 676-6…
    Promoter
    CMV
    Available Since
    July 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    237480-plasmid-map-sequence-id-477747

    Enlarge

  11. KAP95-FLU (113)

    Plasmid
    #24045
    Depositor
    Michael Rout
    Insert
    KAP95 (KAP95 Budding Yeast)
    Use
    Tags
    FLU
    Expression
    Yeast
    Mutation
    Promoter
    Available Since
    March 29, 2010
    Availability
    Academic Institutions and Nonprofits only

  12. KAP123-FLU (116)

    Plasmid
    #24048
    Depositor
    Michael Rout
    Insert
    KAP123 (KAP123 Budding Yeast)
    Use
    Tags
    FLU
    Expression
    Yeast
    Mutation
    Promoter
    Available Since
    March 29, 2010
    Availability
    Academic Institutions and Nonprofits only

  13. KAP121-FLU (115)

    Plasmid
    #24047
    Depositor
    Michael Rout
    Insert
    KAP121 (PSE1 Budding Yeast)
    Use
    Tags
    FLU
    Expression
    Yeast
    Mutation
    Promoter
    Available Since
    March 29, 2010
    Availability
    Academic Institutions and Nonprofits only

  15. pREF-GFP kan

    Plasmid
    #122061
    Purpose
    reporter plasmid for termination and terminator override to express GFP. Marked with URA3
    Depositor
    Daniel Reines
    Article
    Reines et al Methods. 2019 Jan 4. pii: S1046-2023(18)
    Inserts
    intergenic terminator from yeast IMD2
    green fluorescent protein
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL1 comes in pYES2
    Available Since
    Feb. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    122061-plasmid-map-sequence-id-236157

    Enlarge

  20. pEFEX1-A2aGH

    Plasmid
    #160543
    Purpose
    pYES2 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.
    Depositor
    Michelle O'Malley
    Article
    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:
    Insert
    Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    N/A
    Promoter
    Available Since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    160543-plasmid-map-sequence-id-317760

    Enlarge

Showing: 81 - 100 of 137 results