2,366 results
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AE-Polyubiquitin-GCaMP6s
Plasmid#106868PurposeExpresses polyubiquitin GCaMP6s, a genetically encoded calcium indicator, for live calcium imaging in Aedes aegypti mosquitoes.DepositorInsertAE-Polyubiquitin-GCAaMP6s
ExpressionInsectPromoterAe. aegypti polyubiquitin (PUb)Available SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
mApple-PSD95-N-14
Plasmid#54941PurposeLocalization: Membrane Scaffold, Excitation: 568, Emission: 592DepositorInsertPSD95
TagsmAppleExpressionMammalianPromoterCMVAvailable SinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEF6 rglut1 flag complete
Plasmid#89571Purposerglut1 flag completeDepositorAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pORANGE Shank2-GFP KI
Plasmid#131496PurposeEndogenous tagging of Shank2: C-terminal (amino acid position: STOP codon)DepositorAvailable SinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-SnFR-gamma2-minWPRE
Plasmid#165497PurposeAAV for glutamate reporter fused to TARP gamma-2 for postsynaptic localisationDepositorInsertiGluSnFR extracellular domain fused to Stargazin (gamma-2) via NETO2 TM domain (Cacng2 Rat, Synthetic)
UseAAVPromoterhuman SynapsinAvailable SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBa-FRB-3myc-KIF1B beta tail 387-1770
Plasmid#64287PurposeKIF1B beta aa 387-1770 with N-terminal FRB and triple myc tagsDepositorInsertKIF1B beta (Kif1b Rat, Mouse)
Tags3myc and FRBExpressionMammalianMutationaa 387-1770, small portion of 5'end matches …Promoterchicken Beta ActinAvailable SinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
mEos2-Lysosomes-20
Plasmid#57397PurposeLocalization: Lysosome Membrane, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertLAMP1
TagsmEos2ExpressionMammalianMutationD50EPromoterCMVAvailable SinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Cherry-HA-KCC2
Plasmid#104077PurposeTo visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)DepositorInsertKCC2 (b isoform) (Slc12a5 Rat)
TagsmCherry-HA tagExpressionMammalianMutationEcoR1 site in rat KCC2 was removed by silent muta…PromoterUbCAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOC1 Halo-Actb KI
Plasmid#183426PurposeCreON knock-in for Halo-beta-actin (amino acid position: before start codon)DepositorInsertgRNA and Halo donor
UseCRISPRAvailable SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFSynWSyt1C2AB(80-421)msGFP
Plasmid#209014PurposeOverexpression of truncated syt1 tagged with GFP in mammalian cellsDepositorAvailable SinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CG2-Neurog1-Magneto2.0-p2A-mCherry-pA
Plasmid#74302PurposeExpression of Magneto2.0-p2A-mCherry in Rohon-Beard sensory neurons of the zebrafishDepositorUseZebrafishTagsFLAG, cmcl2::EGFP, and p2A-mCherryMutationTRPV4: delta 760-871PromoterNeurog1Available SinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
SnFR-gamma8
Plasmid#165496PurposeGreen fluorescent glutamate reporter fused to TARP gamma-8 for mammalian expression.DepositorInsertiGluSnFR extracellular domain fused to gamma-8 via NETO2 TM domain (Cacng8 Rat, Synthetic)
ExpressionMammalianPromoterCMVAvailable SinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
FKBP-AP180-GFP
Plasmid#186577PurposeExpression of the fusion protein FKBP-AP180-GFP. Uses rat AP180 residues 328-896.DepositorAvailable SinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCR8-Magneto2.0-p2A-mCherry
Plasmid#74334PurposeFor gateway cloning Magneto2.0-p2A-mCherry into destination vectorDepositorUseGateway vectorTagsFlag and p2A-mCherryMutationTRPV4: delta 760–871PromoternoneAvailable SinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-2xTS
Plasmid#109419PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
TagsBAP, HA, His6, T7, and Tyrosine sulfation (TS) se…ExpressionBacterialPromoterT7Available SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ACBEmax
Plasmid#139106PurposeMammalian ACBEmax expression vector (pSI-632)DepositorInsertBi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS
UseCRISPRExpressionMammalianPromoterCMV promoterAvailable SinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO-sh-Nix
Plasmid#100770PurposeLentiviral expression of shRNA targeting NixDepositorInsertLenti-sh-Nix
UseLentiviralExpressionMammalianPromoterhU6Available SinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
RIP7-RLuc-YFP-pBS
Plasmid#72482PurposeExpresses a renilla luciferase-YFP fusion protein under control of the rat insulin 2 gene promoter (RIP7) suitable for transfection or generation of transgenic animalsDepositorInsertsUseLuciferaseTagseYFP and renilla luciferaseExpressionMammalianMutationeYFP varientAvailable SinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct(delta1-6)-mRuby3
Plasmid#155223PurposeF-actin bidning mutant of MPActDepositorInsertMutated version of F-tractin (aa 15-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
TagsmRuby3ExpressionMammalianMutationDeletion of first 6 AA from F-tractin (9-14 from …Available SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only
