2,226 results

1. pCMV5M/rDlg1

   Plasmid

   #28259

   Depositor

   Jeffrey Frost

   Article

   Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

   Insert

   Dlg1 I3
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 14, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pCMV-dCpf1-BE-YE

   Plasmid

   #107686

   Purpose

   Expresses dLbCpf1-BE-YE in mammalian cells

   Depositor

   Jia Chen

   Article

   Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   dLbCpf1-BE-YE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   APOBEC1(W90Y/R126E), dLbCpf1(D832A/E925A/D1148A)

   Promoter

   CMV

   Available since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. tdTurboRFP-Lysosomes-20

   Plasmid

   #58061

   Purpose

   Localization: Lysosome Membrane, Excitation: 553, Emission: 576

   Depositor

   Michael Davidson

   Insert

   LAMP1
   

   Use

   Tags

   tdTurboRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 4, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pcDNA3 βarr1 S412D Flag

   Plasmid

   #42196

   Depositor

   Robert Lefkowitz

   Article

   Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

   Insert

   β-arrestin 1 S412D (Arrb1 Rat)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S412D

   Promoter

   CMV

   Available since

   Jan. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-syn-SnFR-gamma8-minWPRE

   Plasmid

   #165498

   Purpose

   AAV for glutamate reporter fused to TARP gamma-8

   Depositor

   Andrew Plested

   Article

   Hao et al eLife. 2023 Jan 9;12:e84029. doi: 10.755

   Insert

   iGluSnFR extracellular domain fused to TARP gamma-8 via NETO2 TM domain (Cacng8 Synthetic, Rat)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSynapsin

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8s-p10

   Plasmid

   #218865

   Purpose

   LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

   Insert

   RSET-jGCaMP8s
   

   Use

   Tags

   6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

   Expression

   Insect

   Mutation

   S26M F286Y Q315H

   Promoter

   hsp70

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. MBP-rPKM2 WT

   Plasmid

   #107158

   Purpose

   MBP-rPKM2 WT for production and purification of rat PKM2 WT

   Depositor

   Jesse Rinehart

   Article

   Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

   Insert

   MBP-tagged rat pyruvate kinase M2 isoform (Pkm Rat)
   

   Use

   Tags

   C-term 6-his and N-term MBP

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-eBE-S3

   Plasmid

   #101093

   Purpose

   Expresses eBE-S3 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

   Insert

   eBE-S3
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28b-BE3∆UGI

   Plasmid

   #96943

   Purpose

   The plasmid encoding the His6-rAPOBEC1-XTEN-nCas9 protein (BE3∆UGI) was generated by site-directed mutagenesis using pET28b-BE1 (Addgene plasmid #73018).

   Depositor

   Daesik Kim

   Article

   Kim et al Nat Biotechnol. 2017 May;35(5):475-480.

   Insert

   BE3∆UGI
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOC2 Tubb3-GFP KI

    Plasmid

    #183432

    Purpose

    CreOFF knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and GFP donor
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Lenti-evoA1-BE4max

    Plasmid

    #174700

    Purpose

    Base Editing

    Depositor

    Rahul Kohli

    Article

    Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

    Insert

    Lenti-evoA1-BE4max
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mPlum-Lysosomes-20

    Plasmid

    #55982

    Purpose

    Localization: Lysosome Membrane, Excitation: 590, Emission: 649

    Depositor

    Michael Davidson

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Tags

    mPlum

    Expression

    Mammalian

    Mutation

    D50E

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-dCpf1-eBE-YE

    Plasmid

    #107689

    Purpose

    Expresses dLbCpf1-eBE-YE in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-eBE-YE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    APOBEC1(W90Y/R126E), dLbCpf1(D832A/E925A/D1148A)

    Promoter

    CMV

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA

    Plasmid

    #74306

    Purpose

    Bicistronic Cre-dependent expression of TRPV4 & ferritin in mammalian cells

    Depositor

    Ali Guler

    Article

    Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

    Inserts

    TRPV4 (Trpv4 Rat)
    ferritin (FTL and HTL1) (FTL, FTH1 Human)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG and p2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (after cre recombination)

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV5-rat ERK2-K52R

    Plasmid

    #40813

    Depositor

    Natalie Ahn

    Article

    Emrick et al Proc Natl Acad Sci U S A. 2006 Nov 28;10

    Insert

    MAPK1 (Mapk1 Rat)
    

    Use

    Tags

    His6

    Expression

    Mammalian

    Mutation

    K52R

    Promoter

    CMV

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

16. sq5

    Plasmid

    #24499

    Depositor

    Bruce Conklin

    Article

    Conklin et al Mol Pharmacol. 1996 Oct . 50(4):885-90.

    Insert

    Gs alpha (Gnas Rat)
    

    Use

    Tags

    HA (see comments)

    Expression

    Mammalian

    Mutation

    This is Gs alpha with the C-terminal amino acids …

    Promoter

    Available since

    Aug. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-rBcan

    Plasmid

    #18958

    Depositor

    Mariano Viapiano

    Article

    Viapiano et al J Neurooncol. 2008 Jul . 88(3):261-72.

    Insert

    Brevican (Bcan Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

18. Myr.PKCzeta.FLAG

    Plasmid

    #10802

    Depositor

    Alex Toker

    Article

    Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

    Insert

    PKC zeta (Prkcz Rat)
    

    Use

    Tags

    FLAG and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pEGFP-N1-VAPA(1-242) K87D/M89D

    Plasmid

    #18875

    Depositor

    Axel Brunger

    Article

    Kaiser et al Structure. 2005 Jul . 13(7):1035-45.

    Insert

    VAP-A (Vapa Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    double point mutant K87D / M89D

    Promoter

    Available since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pMRX-IP-GFP-LC3-mRuby3

    Plasmid

    #184898

    Purpose

    Stable expression of GFP-LC3-mRuby3 in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Okawa et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    LC3B
    

    Use

    Tags

    EGFP and mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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