Addgene

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pSIBR003

Plasmid

#177666

Purpose

SIBR Intron 3 (Int3) interrupts the CDS of FnCas12a

Depositor

John van der Oost

Article

Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

lacUV5

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-FOXP2

Plasmid

#153146

Purpose

Expression of Flag tagged human FOXP2 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXP2 (FOXP2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-FOXC2

Plasmid

#153115

Purpose

Expression of Flag tagged human FOXC2 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXC2 (FOXC2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-FOXN1

Plasmid

#153137

Purpose

Expression of Flag tagged human FOXN1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXN1 (FOXN1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pAL175

Plasmid

#22276

Purpose

Expresses YFP-PIF for red-light-induced binding to Phy

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

PIF6APB (PIL2 Mustard Weed)

Use

Synthetic Biology

Tags

mYFP

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pISH-Barx1

Plasmid

#84306

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Barx1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Barx1 (Barx1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only

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Flag-FOXD1

Plasmid

#153116

Purpose

Expression of Flag tagged human FOXD1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXD1 (FOXD1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-FOXL2

Plasmid

#153135

Purpose

Expression of Flag tagged human FOXL2 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXL2 (FOXL2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5-ActRIIB

Plasmid

#13501

Depositor

Jeff Wrana

Article

Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

Insert

Activin receptor IIB (Acvr2b Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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34a_PU1 in pmiRGlo

Plasmid

#78131

Purpose

luciferase reporter for miRNA activity

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

PU1

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

3'UTR containing miRNA binding sites

Promoter

PGK

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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Flag-FOXQ1

Plasmid

#153149

Purpose

Expression of Flag tagged human FOXQ1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXQ1 (FOXQ1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-FOXF2

Plasmid

#153123

Purpose

Expression of Flag tagged human FOXF2 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXF2 (FOXF2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-FOXJ3

Plasmid

#153131

Purpose

Expression of Flag tagged human FOXJ3 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXJ3 (FOXJ3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-FOXF1

Plasmid

#153122

Purpose

Expression of Flag tagged human FOXF1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXF1 (FOXF1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-FOXD3

Plasmid

#153118

Purpose

Expression of Flag tagged human FOXD3 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXD3 (FOXD3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLOXp-GUS

Plasmid

#154059

Purpose

Level 0.5 Golden Gate vector, loxP-GUS-nosT-loxP

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

beta-glucoronidase (E. coli)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

N/A

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pAL3

Plasmid

#114673

Purpose

T7 vector having very low basal expression of target protein can clone and express almost any gene. Asymmetric ligation clones as many as 3 genes simultaneously for co-expression from the same mRNA.

Depositor

William Studier

Article

Studier et al Curr Protoc Mol Biol. 2018 Oct;124(1):e6

Type

Empty backbone

Use

Tags

optional Ser-Gly-6His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pTetR-BCD20-mCherry-ssrA P66U23C90T20 v40-3a (588)

Plasmid

#70745

Purpose

for building 3-color reporter plasmid

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

mCherry-ssrA

Use

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Chronos-mRuby2-ST

Plasmid

#105446

Purpose

Cation channelrhodopsin Chronos fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:

Insert

Chronos-mRuby2-ST

Use

Tags

mRuby2 and soma targeting motif

Expression

Mutation

Promoter

pCAGGS

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Tshz1

Plasmid

#74360

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Tshz1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

teashirt zinc finger family member 1 (Tshz1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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