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Plasmid#12394DepositorInsertPL1
UseTags6xHisExpressionBacterialMutationRosettaDesign was used to design sequences. Align…PromoterAvailable sinceMarch 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_FL Gcn4
Plasmid#232955PurposeGalactose iduced expression of full-length Gcn4 in yeastDepositorInsertGcn4
UseTagsTEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_DBDHA
Plasmid#232987PurposeGalactose iduced expression of Gcn4 DBDAHA in yeastDepositorInsertDBD
UseTags3xHA and TEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 WT 44merHA
Plasmid#232988PurposeGalactose iduced expression of Gcn4 WT 44merHA in yeastDepositorInsertGcn4 WT 44mer
UseTags3xHA and TEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 L123A
Plasmid#232969PurposeGalactose iduced expression of Gcn4 L123A in yeastDepositorInsertGcn4 L123A
UseTagsTEV cleavage siteExpressionYeastMutationL123APromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Swap2
Plasmid#232965PurposeGalactose iduced expression of Gcn4 k0.19 in yeastDepositorInsertGcn4 k0.19
UseTagsTEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 K5
Plasmid#232966PurposeGalactose iduced expression of Gcn4 K+ in yeastDepositorInsertGcn4 K+
UseTagsTEV cleavage siteExpressionYeastMutationD103K, D115K, D127K, D133K, E143KPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Swap3
Plasmid#232960PurposeGalactose iduced expression of Gcn4 k0.47 in yeastDepositorInsertGcn4 k0.47
UseTagsTEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 R2
Plasmid#232970PurposeGalactose iduced expression of Gcn4 R+ in yeastDepositorInsertGcn4 R+
UseTagsTEV cleavage siteExpressionYeastMutationK118R, K140RPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 E+
Plasmid#232963PurposeGalactose iduced expression of Gcn4 E+in yeastDepositorInsertGcn4 E+
UseTagsTEV cleavage siteExpressionYeastMutationD105E, D118E, D139EPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 W120A
Plasmid#232962PurposeGalactose iduced expression of Gcn4 W120A in yeastDepositorInsertGcn4 W120A
UseTagsTEV cleavage siteExpressionYeastMutationW120APromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 R3
Plasmid#232973PurposeGalactose iduced expression of Gcn4 R++ in yeastDepositorInsertGcn4 R++
UseTagsTEV cleavage siteExpressionYeastMutationD103R, D133R, K143RPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Swap3GFP
Plasmid#233010PurposeGalactose iduced expression of Gcn4 k0.47GFP in yeastDepositorInsertGcn4 k0.47
UseTagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_DBD
Plasmid#232985PurposeGalactose iduced expression of Gcn4 DBD in yeastDepositorInsertGcn4 DBD
UseTagsTEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 F124I
Plasmid#232964PurposeGalactose iduced expression of Gcn4 F124I in yeastDepositorInsertGcn4 F124I
UseTagsTEV cleavage siteExpressionYeastMutationF124IPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Swap3HA
Plasmid#232998PurposeGalactose iduced expression of Gcn4 k0.47HA in yeastDepositorInsertGcn4 k0.47
UseTags3xHA and TEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 WT 44mer
Plasmid#232956PurposeGalactose iduced expression of Gcn4 WT 44mer in yeastDepositorInsertGcn4 WT 44mer
UseTagsTEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 WW+
Plasmid#232984PurposeGalactose iduced expression of Gcn4 WW+ in yeastDepositorInsertGcn4 WW+
UseTagsTEV cleavage siteExpressionYeastMutationE109N, S117D, T121W, N126W, T132WPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_DBDGFP
Plasmid#232999PurposeGalactose iduced expression of Gcn4 DBDGFP in yeastDepositorInsertDBD
UseTagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 WT 44merGFP
Plasmid#233000PurposeGalactose iduced expression of Gcn4 WT 44merGFP in yeastDepositorInsertGcn4 WT 44mer
UseTagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only