We narrowed to 2,455 results for: GEM

1. pCIneo-lambdaN-HA-CeSmg5-Q94E_S

   Plasmid

   #147459

   Purpose

   Mammalian Expression of CeSmg5-Q94E

   Depositor

   Elisa Izaurralde

   Article

   Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

   Insert

   CeSmg5-Q94E (smg-5 Nematode)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCIneo-V5-SBP-CeSmg7_S

   Plasmid

   #147465

   Purpose

   Mammalian Expression of CeSmg7

   Depositor

   Elisa Izaurralde

   Article

   Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

   Insert

   CeSmg7 (smg-7 Nematode)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCIneo-V5-SBP-CeSmg7-G80E_S

   Plasmid

   #147466

   Purpose

   Mammalian Expression of CeSmg7-G80E

   Depositor

   Elisa Izaurralde

   Article

   Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

   Insert

   CeSmg7-G80E (smg-7 Nematode)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCIneo-V5-SBP-CeSmg7-T83E_S

   Plasmid

   #147467

   Purpose

   Mammalian Expression of CeSmg7-T83E

   Depositor

   Elisa Izaurralde

   Article

   Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

   Insert

   CeSmg7-T83E (smg-7 Nematode)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX-6P-1-CeSMG5_1-420_S

   Plasmid

   #147468

   Purpose

   Bacterial Expression of CeSMG5_1-420

   Depositor

   Elisa Izaurralde

   Article

   Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

   Insert

   CeSMG5_1-420 (smg-5 Nematode)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRSFDuetMP-CeSmg7_1-395_S

   Plasmid

   #147475

   Purpose

   Bacterial Expression of CeSmg7_1-395

   Depositor

   Elisa Izaurralde

   Article

   Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

   Insert

   CeSmg7_1-395 (smg-7 Nematode)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCIneo-lambdaN-HA-CeSmg5-H116E_S

   Plasmid

   #147458

   Purpose

   Mammalian Expression of CeSmg5-H116E

   Depositor

   Elisa Izaurralde

   Article

   Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

   Insert

   CeSmg5-H116E (smg-5 Nematode)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p005-RFP-weak

   Plasmid

   #108316

   Purpose

   Synthetic RFP based on Corynactis spp under weak synthetic constitutive promoter pFAB4024; kanamycin resistant.

   Depositor

   Jaroslaw Bryk

   Article

   Siddall et al bioRxiv 2021.06.20.449138

   Insert

   RFP under controlf of weak synthetic constitutive promoter pFAB4024
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pFAB4024

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBP-BBa_J23100

   Plasmid

   #74097

   Purpose

   BBa_J23100 promoter (iGEM collection)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Bba_J23100 constitutive promoter (Sigma70)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   None

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCas8

    Plasmid

    #82380

    Purpose

    dCas9 without a promoter in acsA

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCas73

    Plasmid

    #82398

    Purpose

    Δccm:Km, used to delete ccm operon

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    ccm deletion
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBP-BBa_B0012

    Plasmid

    #72997

    Purpose

    BBa_B0012 iGEM terminator (5' TCGA/ 3' TGTT fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Bba_B0012 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBP-BBa_B0015

    Plasmid

    #72998

    Purpose

    BBa_B0015 iGEM terminator (5' TCGA/ 3' TGTT fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    BBa_B0015 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBP_BBa_B0034

    Plasmid

    #72980

    Purpose

    Bba_B0034 iGEM RBS (5' GTAC/3' CATA fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Bba_B0034 RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pK18sB

    Plasmid

    #177838

    Purpose

    Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size and lacking oriT required for conjugation.

    Depositor

    Gregg Beckham, Christopher Johnson

    Article

    Jayakody et al Energy Environ. Sci., 2018,11, 1625-1638

    Type

    Empty backbone

    Use

    Bacterial gene replacement

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEF1-alpha-V

    Plasmid

    #27290

    Depositor

    Timothy Springer

    Article

    Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

    Insert

    integrin alpha V (ITGAV Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Isoform 2

    Promoter

    Available since

    March 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TetOn-mCherry-eGFP-RAMP4

    Plasmid

    #109014

    Purpose

    Tet-On construct to induce expression of tandem eGFP-mCherry tagged RAMP4 (ER marker)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    RAMP4 (SERP1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry-eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA

    Plasmid

    #63166

    Purpose

    GAL4-responsive UAS "flp-On" spaghetti monster GFP-HA reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-HA"
    

    Use

    Tags

    N-myristoylation

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. tubP-FRT>GAL80-6-FRT>

    Plasmid

    #63173

    Purpose

    ubiquitous expression of a "flp-Out" Drosophila codon-optimized GAL80 gene cassette

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "Flp-Out" GAL80
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCI-SEP_NR2B

    Plasmid

    #23998

    Depositor

    Robert Malinow

    Article

    Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

    Insert

    NR2B (Grin2b Rat)
    

    Use

    Tags

    NR2B signal sequence (aa1-31) and SEP (supereclip…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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