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Showing: 981 - 1000 of 13379 results

  2. pAAV Syn ChR E90R-D156N-T159C 2A tDimer

    Plasmid
    #52494
    Purpose
    Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)
    Depositor
    Thomas Oertner
    Article
    Wietek et al Science. 2014 Mar 27.
    Inserts
    Channelrhodopsin-2
    red fluorescent protein
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    E90R, D156N, T159C
    Promoter
    human synapsin-1 and ribosomal skip sequence
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    52494-plasmid-map-sequence-id-81429

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  3. pAAV Syn ChR E90R-T159C 2A tDimer

    Plasmid
    #52495
    Purpose
    Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)
    Depositor
    Thomas Oertner
    Article
    Wietek et al Science. 2014 Mar 27.
    Inserts
    Channelrhodopsin-2
    red fluorescent protein
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    E90R, T159C
    Promoter
    human synapsin-1 and ribosomal skip sequence
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    52495-plasmid-map-sequence-id-81430

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  9. FB012

    Plasmid
    #119709
    Purpose
    Complete TU for hygromicin resistance in fungi, domesticated into pUPD2 with AATG/GCTT barcodes. Used for gene KO with dual selection, according to FungalBraid modular DNA assembly for ATMT
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    PtrpC:hph:Ttub
    Use
    Synthetic Biology; Domestication of dna parts for…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119709-plasmid-map-sequence-id-237149

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  11. pENTR-B/c

    Plasmid
    #227962
    Purpose
    Entry plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.
    Depositor
    Alberto Carbonell
    Article
    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka
    Insert
    B/c
    Use
    Tags
    Expression
    Mutation
    Promoter
    None
    Available since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    227962-plasmid-map-sequence-id-454806

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  12. pMDC32B-B/c

    Plasmid
    #227963
    Purpose
    Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.
    Depositor
    Alberto Carbonell
    Article
    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka
    Insert
    B/c
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    227963-plasmid-map-sequence-id-454828

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  13. pRAR051

    Plasmid
    #185029
    Purpose
    400 nt l31 promoter region, l31 5'UTR with nt. 47-51 and 80-84 mutated, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB
    Depositor
    Julius Lucks
    Article
    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127
    Insert
    L31-sfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Nucleotides 47-51 & 80-84 mutated rpmE 5'…
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    185029-plasmid-map-sequence-id-363327

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  14. pRAR052

    Plasmid
    #185030
    Purpose
    400 nt l31 promoter region, l31 5'UTR with nt. 56-61 and 68-73 mutated, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB
    Depositor
    Julius Lucks
    Article
    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127
    Insert
    L31-sfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Nucleotides 56-61 and 68-73 mutated in rpmE 5…
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    185030-plasmid-map-sequence-id-366943

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  15. pRAR064

    Plasmid
    #185031
    Purpose
    400 nt l31 promoter region, scrambled 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB
    Depositor
    Julius Lucks
    Article
    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127
    Insert
    L31-sfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Nucleotides of rpmE 5'UTR scrambled, Only fi…
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    185031-plasmid-map-sequence-id-373311

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  17. pRAR102

    Plasmid
    #185033
    Purpose
    400 nt l31 promoter region, l31 5'UTR U54C mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB
    Depositor
    Julius Lucks
    Article
    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127
    Insert
    L31-sfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    U54C mutation of rpmE 5'UTR, Only first 90 n…
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    185033-plasmid-map-sequence-id-366954

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  18. pRAR106

    Plasmid
    #185036
    Purpose
    400 nt l31 promoter region, l31 5'UTR G79U mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB
    Depositor
    Julius Lucks
    Article
    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127
    Insert
    L31-sfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    G79U mutation of rpmE 5'UTR, Only first 90 n…
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    185036-plasmid-map-sequence-id-374745

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  19. pRAR116

    Plasmid
    #185040
    Purpose
    400 nt l31 promoter region, l31 5'UTR U58C and U70C, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB
    Depositor
    Julius Lucks
    Article
    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127
    Insert
    L31-sfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    U58C and U70C of rpmE of 5'UTR, Only first 9…
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    185040-plasmid-map-sequence-id-363333

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Showing: 981 - 1000 of 13379 results