We narrowed to 15,398 results for: nans

1. P-rTetO-mCherry-T2A-RPL22-1xV5

   Plasmid

   #170325

   Purpose

   Tetracycline-inducible expression of V5 and mCherry tagged RPL22

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSET-XS-VWF

   Plasmid

   #64847

   Purpose

   Expresses aa1594–1670 of VWF A2 domain flanked by Venus and Cerulean and tagged with 7X His at C terminus

   Depositor

   Sriram Neelamegham

   Article

   Dayananda et al Anal Biochem. 2011 Mar 15;410(2):206-13.

   Insert

   Venus_VWF A2 domain aa 1594-1670_Cerulean_TEV_7X His (VWF Human, Synthetic)
   

   Use

   Tags

   7X His, Cerulean, and Venus

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYpet-Optop38i

   Plasmid

   #89749

   Purpose

   Expression of light-regulated p38 inhibitor, Ypet-fused, wild-type photosensor, inhibitor MK3BD3-13F

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   Optop38i3
   

   Use

   Fluorescent protein

   Tags

   Ypet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmCherry-3xNLS-OptoJNKi

   Plasmid

   #89740

   Purpose

   Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, wild-type photosensor, inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   NLS-OptoJNKi
   

   Use

   Fluorescent protein

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKCAG-mCherry-OptoJNKi

   Plasmid

   #89738

   Purpose

   Expression of light-regulated JNK inhibitor, wild-type photosensor, inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   OptoJNKi
   

   Use

   Fluorescent protein

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmCherry-NES-OptoJNKi

   Plasmid

   #89739

   Purpose

   Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to cytoplasm, wild-type photosensor, inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   NLS-OptoJNKi
   

   Use

   Fluorescent protein

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKCAG-H2BmCherry-OptoJNKi

   Plasmid

   #89741

   Purpose

   Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to chromatin with H2B, wild-type photosensor, inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   OptoJNKi
   

   Use

   Optogenetics

   Tags

   Histone2B and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQCXIH FRB-BFP

   Plasmid

   #202425

   Purpose

   TagBFP FKBP-rapamycin binding (FRB) dimerization domain

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

   Insert

   FKBP-rapamycin binding (FRB) dimerization domain
   

   Use

   Lentiviral

   Tags

   TagBFP

   Expression

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDECKO_mCherry_KLF4

   Plasmid

   #140104

   Purpose

   CRISPR-Cas9 library validation

   Depositor

   Roderic Guigo

   Article

   Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   gRNA1 under U6 and gRNA2 under H1

   Available since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLuc-OptoJNKi

    Plasmid

    #89744

    Purpose

    Expression of light-regulated JNK inhibitor, firefly luciferase-fused, wild-type photosensor, inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    OptoJNKi
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. lenti-EF1a-dCas9-VPR-Puro

    Plasmid

    #99373

    Purpose

    3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    (Sp)dCas9-VPR-P2A-Puro
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQCXIZ GFP PODXL A WT

    Plasmid

    #202413

    Purpose

    Expression of GFP-tagged PODXL (isoform A) WT

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    PODXL (PODXL Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQCXIZ GFP PODXL B WT

    Plasmid

    #202414

    Purpose

    Expression of GFP-tagged PODXL (isoform B) WT

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    PODXL (PODXL Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pmCherry-N1 GBP (GBP-mCherry)

    Plasmid

    #162879

    Purpose

    Expression in mammalian cells of GFP binding protein (GBP) tagged with mCherry

    Depositor

    Frederic Meunier

    Article

    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

    Insert

    GFP Binding Protein
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEAT8-137 M1V

    Plasmid

    #173807

    Purpose

    Expression and purification of mature (no signal sequence) human alpha1-antitrypsin wildtype variant M1V (UniProt P01009) from E. coli BL21(DE3) cells

    Depositor

    Anne Gershenson

    Article

    Krishnan et al Methods Mol Biol. 2017;1639:195-209. doi

    Insert

    SERPiNA1 (SERPINA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E1M Otherwise this plasmid encodes the most commo…

    Promoter

    T7

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMXs-LN2

    Plasmid

    #188235

    Purpose

    Polycistronic human LIN28 and NANOG were cloned into the pMXs vector

    Depositor

    Young Tang

    Article

    Wang et al Biol Open. 2019 Dec 5;8(12). pii: 8/12/b

    Inserts

    LIN28A (LIN28A Human)
    NANOG (NANOG Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMXs-LN

    Plasmid

    #188231

    Purpose

    Polycistronic human LIN28 and NANOG were cloned into the pMXs vector

    Depositor

    Young Tang

    Article

    Wang et al Biol Open. 2019 Dec 5;8(12). pii: 8/12/b

    Insert

    LIN28A and NANOG separated by 2A sequence (LIN28A, NANOG Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pmCherry-3xNLS-OptoJNKi-dsm

    Plasmid

    #89743

    Purpose

    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    NLS-OptoJNKi dark-state mutant
    

    Use

    Fluorescent protein

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    C450A mutation of AsLov2Jalpha

    Promoter

    CMV

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmCherry-3xNLS-OptoJNKi-lsm

    Plasmid

    #89742

    Purpose

    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    NLS-OptoJNKi lit-state mutant
    

    Use

    Fluorescent protein

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    I539E mutation of AsLov2Jalpha

    Promoter

    CMV

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQCXIH FRB Ubiqutin-BFP

    Plasmid

    #202426

    Purpose

    TagBFP FKBP-rapamycin binding (FRB) dimerization domain fused to ubiquitin

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    FKBP-rapamycin binding (FRB) dimerization domain
    

    Use

    Lentiviral

    Tags

    Ubiquitin, TagBFP

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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