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Anti-SAPAP1/2 [N238/31R]

Plasmid

#182110

Purpose

Mammalian Expression Plasmid of anti-SAPAP1/2 (). Derived from hybridoma N238/31.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-SAPAP1/2 () recombinant mouse monoclonal antibody

Expression

Mammalian

Promoter

Dual CMV

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-NDUFA10-FKBP-HA

Plasmid

#185810

Purpose

NDUFA10 subunit of Complex I fused with C-terminal FKBP and HA epitope

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFA10 (NDUFA10 Human)

Use

Lentiviral

Tags

FKBP and HA

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFUGW-shRIIα-RIIαS97A-IRES-EGFP

Plasmid

#183457

Purpose

Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97A variant

Depositor

Matthew Gold

Article

Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

Insert

sgRNA and Prkar2a (Prkar2a Rat)

Use

Lentiviral

Mutation

shRNA-resistant mutations: C135>G, G138>A, …

Promoter

shRNA: H1 / gene: ubiquitin

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pFUGW-shRIIα-RIIαS97E-IRES-EGFP

Plasmid

#183458

Purpose

Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97E variant

Depositor

Matthew Gold

Article

Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

Insert

sgRNA and Prkar2a (Prkar2a Rat)

Use

Lentiviral

Mutation

shRNA-resistant mutations: C135>G, G138>A, …

Promoter

shRNA: H1 / gene: ubiquitin

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-SopB(172-554)

Plasmid

#183664

Purpose

N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 172-554

Promoter

CMV

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-SopB(211-554)

Plasmid

#183665

Purpose

N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 211-554

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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Parvalbumin scFv [L114/81]

Plasmid

#182073

Purpose

Mammalian Expression of Parvalbumin scFv. Derived from hybridoma L114/81.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Parvalbumin (Rattus norvegicus) (Pvalb Mouse)

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-TRIP8b (constant) [N212/7R]

Plasmid

#177514

Purpose

Mammalian Expression Plasmid of anti-TRIP8b (constant) (Rat). Derived from hybridoma N212/7.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-TRIP8b (constant) (Rattus norvegicus) recombinant mouse monoclonal antibody (Pex5l Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA4 3xFLAG Halo E55A E57A K58A nsp1

Plasmid

#175427

Purpose

Expresses E55A E57A K58A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

nsp1 E55A E57A K58A

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

N-terminal mutants E55A E57A K58A

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAW-BiP-Nb127D01-mCherry-KDEL

Plasmid

#171575

Purpose

Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cell ER membrane

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

BiP-Nb127D01-mCherry-KDEL (CXCR2 Human)

Tags

BiP signal peptide and mCherry-KDEL

Expression

Insect

Promoter

fly actin5C promoter

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAW-BiP-NbVHH05-mCherry-KDEL

Plasmid

#171574

Purpose

Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cell ER membrane

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

BiP-NbVHH05-mCherry-KDEL (UBE2J1 Human)

Tags

BiP signal peptide and mCherry-KDEL

Expression

Insect

Promoter

fly actin5C promoter

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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p35S_GFP-EVDinter-GUS

Plasmid

#167123

Purpose

Plant binary expression vector containing the intron and proximal polyadenylation signal sequences of the Copia93 retroelement EVADE (AT5G17125) between mGFP5 and GUS under CaMV 35S promoter.

Depositor

Arturo Marí-Ordóñez, Olivier Voinnet

Article

Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

Insert

EVADE GAG intron and terminator (EVD_in/ter) (AT5G17125 Mustard Weed)

Tags

GUS and mGFP5

Expression

Plant

Promoter

CaMV 35S

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV ShadowY-AURKA-mTurq2

Plasmid

#157770

Purpose

Expression of AuroraA kinase biosensor under CMV promoter

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Tags

mTurquoise2 and shadowY

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-TRIP8b [N212/7R]

Plasmid

#164154

Purpose

Mammalian Expression Plasmid of anti-TRIP8b (Rat). Derived from hybridoma N212/7.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-TRIP8b (Rattus norvegicus) recombinant mouse monoclonal antibody (Pex5l Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Iduna/RNF146 [N201/35R]

Plasmid

#164157

Purpose

Mammalian Expression Plasmid of anti-Iduna/RNF146 (Mouse). Derived from hybridoma N201/35.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Iduna/RNF146 (Mus musculus) recombinant mouse monoclonal antibody (Rnf146 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-ZNF423 [N328B/37R]

Plasmid

#140077

Purpose

Mammalian Expression Plasmid of anti-ZNF423 (Human). Derived from hybridoma N328B/37.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-ZNF423 (Human) recombinant mouse monoclonal antibody (ZNF423 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-N-3HA-GK_v2

Plasmid

#134277

Purpose

Lentivector encoding 3XHA-tagged GK (isoform 2)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk (Gk Mouse)

Use

Lentiviral

Tags

3X HA

Expression

Mammalian

Mutation

553 amino acids, isoform 2

Promoter

CMV

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pSECC-sgMARK4-A

Plasmid

#138676

Purpose

Expresses a mouse MARK4-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgMARK4 mouse (Mark4 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSECC-sgMARK4-B

Plasmid

#138677

Purpose

Expresses a mouse MARK4-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgMARK4 mouse (Mark4 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-REEP1/2 [N326D/29R]

Plasmid

#114510

Purpose

Mammalian Expression Plasmid of anti-REEP1/2 (Mouse). Derived from hybridoma N326D/29.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-REEP1/2 (Mus musculus) recombinant mouse monoclonal antibody (Reep1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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