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Showing: 10081 - 10100 of 16981 results

  2. FB013

    Plasmid
    #119710
    Purpose
    TU of the HSVtk negative marker for F2dU domesticated into pUPD2 with GGAG/TACT barcodes (reverse orientation). Used for gene KO with dual selec. According to FungalBraid modular DNA assembly for ATMT
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Negative selection marker HSVtk (Pgpda:HSVtk:Ttub)
    Use
    Synthetic Biology; Domestication of dna parts for…
    Available Since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119710-plasmid-map-sequence-id-237145

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  6. pLCHKO_TK1_HPRT1_Lb_4

    Plasmid
    #155062
    Purpose
    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155062-plasmid-map-sequence-id-308576

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  8. pAAV_hSyn_HA-TALE(NG)-Tet1FL-NLS_2A_EGFP_W3SL

    Plasmid
    #172195
    Purpose
    AAV sequence-specific control vector for TALE-Tet1FL-SID4X. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.
    Depositor
    Garrett Kaas
    Article
    Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d
    Insert
    HA-TALE(NG)-Tet1FL-NLS_2A_EGFP_W3SL
    Use
    AAV and Mouse Targeting; Tale
    Tags
    HA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    transcription regulatory domain removed
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available Since
    Aug. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    172195-plasmid-map-sequence-id-343564

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  12. pMCh2008_pLenti_Syn_mChe-cdc42E7-E63'UTR

    Plasmid
    #118622
    Purpose
    expression of mChe-tagged cdc42E7-E6-3'UTR under pSyn promoter for primary neurons
    Depositor
    Marina Chekulaeva
    Article
    Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525
    Insert
    cdc42E7-E6 3'UTR (Cdc42 Mouse)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    changed ttgcttt to cggtaag (496 - 502 nt in 3…
    Promoter
    synapsin I (rat)
    Available Since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    118622-plasmid-map-sequence-id-230551

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  15. FLAG-RFP-UCR1C

    Plasmid
    #67582
    Purpose
    Expresses C-terminal amino acids of UCR1 region of PDE4D3 in mammalian cells
    Depositor
    Graeme Carnegie
    Article
    Wang et al Cell Signal. 2015 May;27(5):908-22. doi:
    Insert
    C-terminus of upstream conserved region 1 of phosphodiesterase 4D3 (PDE4D Human)
    Tags
    FLAG and TagRFP
    Expression
    Mammalian
    Mutation
    Amino acid residues 80-136 of PDE4D3
    Available Since
    Oct. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. pDEST-CMV 3xFLAG-LC3B-G120A-GFP

    Plasmid
    #123108
    Purpose
    Expresses 3xFLAG-LC3B-G120A-EGFP in mammalian cells, negative control for monitoring ATG4 activity.
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Insert
    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    Tags
    3xFLAG and EGFP
    Expression
    Mammalian
    Mutation
    Glycine 120 to Alanine
    Promoter
    CMV
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123108-plasmid-map-sequence-id-244364

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Showing: 10081 - 10100 of 16981 results