Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 10,556 results for: aav

Showing: 10121 - 10140 of 10556 results

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pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-6

Plasmid

#129034

Purpose

targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA6 targeting human TSDR

Depositor

Julia K Polansky

Publication

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA6 (huTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-7

Plasmid

#129035

Purpose

targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA7 targeting human TSDR

Depositor

Julia K Polansky

Publication

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA7 (huTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-9

Plasmid

#129037

Purpose

targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA9 targeting human TSDR

Depositor

Julia K Polansky

Publication

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA9 (huTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-10

Plasmid

#129038

Purpose

targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA10 targeting human TSDR

Depositor

Julia K Polansky

Publication

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA10 (huTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-11

Plasmid

#129039

Purpose

targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA11 targeting human TSDR

Depositor

Julia K Polansky

Publication

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA11 (huTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-2

Plasmid

#129030

Purpose

targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA2 targeting human TSDR

Depositor

Julia K Polansky

Publication

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA2 (huTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-3

Plasmid

#129031

Purpose

targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA3 targeting human TSDR

Depositor

Julia K Polansky

Publication

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA3 (huTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-4

Plasmid

#129032

Purpose

targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA4 targeting human TSDR

Depositor

Julia K Polansky

Publication

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA4 (huTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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GGDestX2-Amp

Plasmid

#157654

Purpose

Golden Gate assembly and mammalian expression of dCas9 fusion-proteins plus gRNA

Depositor

Karmella Haynes

Publication

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

U6, CBh

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pKH-1699

Plasmid

#124227

Purpose

Mammalian SpCas9-HF1 expression

Depositor

Ervin Welker

Publication

Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

Insert

SpCas9-HF1

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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PX458-Lck

Plasmid

#159430

Purpose

CRISPR knockout of Lck

Depositor

Arthur Salomon

Publication

Chua et al J Proteome Res. 2020 Nov 13. doi: 10.102

Insert

sgRNA targeting Lck exon 2 (LCK Human)

Use

CRISPR

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX330_gRNA_H19-DMR-2

Plasmid

#159930

Purpose

Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

Depositor

Anton Wutz

Publication

Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

Insert

gRNA mouse H19-DMR (H19-icr Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6, CBh

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330_gRNA_IG-DMR-1

Plasmid

#159931

Purpose

Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

Depositor

Anton Wutz

Publication

Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

Insert

gRNA mouse IG-DMR

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6, CBh

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330_gRNA_H19-DMR-1

Plasmid

#159929

Purpose

Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

Depositor

Anton Wutz

Publication

Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

Insert

gRNA mouse H19-DMR (H19-icr Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6, CBh

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330_gRNA_IG-DMR-2

Plasmid

#159932

Purpose

Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

Depositor

Anton Wutz

Publication

Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

Insert

gRNA mouse IG-DMR

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6, CBh

Available since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-U6+27-Linear-2_PolyU(1)_HairpinDistance(10)nts_Tornado-Corn

Plasmid

#159501

Purpose

Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.

Depositor

Julius Lucks

Publication

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-2_PolyU(1)_HairpinDistance(10)nts

Expression

Mammalian

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-U6+27-Linear-2_PolyU(4)_HairpinDistance(10)nts_Tornado-Corn

Plasmid

#159502

Purpose

Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.

Depositor

Julius Lucks

Publication

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-2_PolyU(4)_HairpinDistance(10)nts

Expression

Mammalian

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-U6+27-Linear-3_PolyU(1)_Tornado-Corn

Plasmid

#159503

Purpose

Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Publication

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-3_PolyU(1)

Expression

Mammalian

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-U6+27-Linear-3_PolyU(1)_HairpinDistance(10)nts_Tornado-Corn

Plasmid

#159504

Purpose

Tests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.

Depositor

Julius Lucks

Publication

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-3_PolyU(1)_HairpinDistance(10)nts

Expression

Mammalian

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(10)nts_Tornado-Corn

Plasmid

#159506

Purpose

Tests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.

Depositor

Julius Lucks

Publication

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-3_PolyU(4)_HairpinDistance(10)nts

Expression

Mammalian

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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