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Plasmid#226544PurposeBacterial expression of N-terminally 6His tagged A1-LCD X2DepositorInsertA1-LCD swap X2
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDA313
Plasmid#195214PurposeExpression of superfolder GFP (sfGFP) by a hybrid T7 promoter with tetO.DepositorInsertsfGFP with tetO and a hairpin
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVG97
Plasmid#164849PurposePlasmid for replacement of PEST sequence in strains with pr-yEVenus-PEST-ADH1t constructDepositorInsertPart of URA3 5' UTR and yEVenus with stop codon for recombination with pr-yEVenus-PEST-ADH1t:URA
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
smHA multi-frame tag
Plasmid#128611Purposea spaghetti monster with 10x HA epitopes repeat upstream of the HIV frameshift sequence which is followed by FLAG epitopes in the 0ORF are separated from one another by SunTag epitopes in the -1ORF.DepositorInsertsmHA MF tag
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICH47742:FCP:Cas9YFP
Plasmid#85986PurposeGolden Gate Level 1 cassette encoding a Cas9-YFP fusion under the FCP promoterDepositorInsertCas9
UseCRISPR; Golden gate assemblyTagsSV40 NLS, YFPExpressionMutationPromoterFCPAvailable sinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL3-fugu SLC2A4 promoter
Plasmid#65101PurposeLuciferase reporter construct under the fugu (Takifugu rubripes) SLC2A4 promoter.DepositorInsertSLC2A4 promoter
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
6xmyc_cort_UAS-P
Plasmid#112991PurposeP element vector for transposon insertion of Myc-tagged Drosophila cort (Cdc20) into Drosophila genomeDepositorInsertcort (Cdc20) (cort Fly)
UseP insertion vectorTags6X myc epitopeExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0062 RanCas13 (B06) His6-TwinStrep-SUMO-BsaI
Plasmid#115213PurposeExpresses RanCas13b from a SUMO-tagged bacterial expression constructDepositorInsertRanCas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLAG-Claspin
Plasmid#12659DepositorInsertClaspin (CLSPN Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAGM4723:TpCC_Urease
Plasmid#85982PurposeGolden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAsDepositorInsertsCas9
Urease-targeting gRNA 1
Urease-targeting gRNA 2
UseCRISPR; Diatom (t. pseudonana)TagsNLS and YFPExpressionMutationPromoterFCP and U6Available sinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaI
Plasmid#115210PurposeExpresses AspCas13b from a SUMO-tagged bacterial expression constructDepositorInsertAspCas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCW57-GFP-miR-302cluster
Plasmid#132549PurposeDoxycycline-inducible expression of human miR-302 clusterDepositorInserthuman miR-302 cluster: MIR302B, MIR302C, MIR302A, MIR302D, MIR367
UseLentiviral; Doxycycline inducible, microrna expre…TagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCA16-Ubc-NLS-HA-dSaCas9-NLS-[10XGCN4]-P2A-BSD
Plasmid#199447PurposeExpession of dSaCas9-10XGCN4 that recruits scFV-tdTomato (pCA15) for amplifying fluorescent signalDepositorInsertdSaCas9-10xGCN4
UseLentiviralTagsExpressionMammalianMutationPromoterhuman ubiquitin C (UBC)Available sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJM31
Plasmid#52986PurposeA secreted Annexin V (sAnxV::GFP) Phosphatidylserine sensorDepositorInsertAnnexin V (ANXA5 Human)
UseTagsGFP (w/ introns) and signal sequenceExpressionWormMutationPromoterhsp-16.41Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJM483
Plasmid#52987PurposeA secreted Lactadherin (sGFP::LactC1C2) Phosphatidylserine sensorDepositorInsertLactaherin (Mfge8 Mouse)
UseTagsGFP (w/ introns) and signal sequenceExpressionWormMutationdeleted native signal sequence, deleted C-termina…Promoterhsp-16.41Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
EC2_2_dCas9_VP64_sgRNA
Plasmid#163707PurposeYeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domainDepositorInsertsdCas9
VP64+SV40 NLS
UseTagsExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM656
Plasmid#53086PurposeA non-Phosphatidylserine binding mutant form of Lactadherin(sGFP::LactC1C2mut) to be used as a controlDepositorInsertLactaherin (Mfge8 Mouse)
UseTagsGFP (w/ introns) and signal sequenceExpressionWormMutationdeleted native signal sequence, deleted C-termina…Promoterhsp-16.41Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
human rSHIP2 (18-893)
Plasmid#128568PurposeExpression of human SHIP2 in bacteriaDepositorInsertSHIP2 (INPPL1 Human)
UseTagsHis Xpress (N-terminal)ExpressionBacterialMutationSHIP2 coding sequence from amino acid 18 (A) till…PromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNoROS-CAT
Plasmid#170152PurposeMammalian expression vector encoding a constitutive nucleolar targeted roGFP and a doxycycline inducible nucleolar targeted catalase. Used to study nucleolar oxidationDepositorInsertsUseGenome integration via sleeping beauty transposon…TagsFLAG tag and Nucleolar Localization Sequence (NoL…ExpressionMammalianMutationPromoterRPBSA and TRE promoterAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
123_pAAV-ProC1-CatCh-GFP-WPRE
Plasmid#125937PurposeTargeting expression to neuronal and glial cell types in mice, non-human primates, and human retinaDepositorInsertCatCh-GFP
UseAAVTagsExpressionMutationPromoterProC1Available sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only