We narrowed to 2,836 results for: try
-
Plasmid#137769PurposeCALCOCO1 LIR mutantDepositorInsertCALCOCO1 W47A
TagsmycDDKExpressionMammalianMutationW47APromoterCMVAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRPa-iSL(MCS1+2)
Plasmid#69244PurposeTet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.DepositorTypeEmpty backboneUseExpression in trypanosoma bruceiPromoterRRNA promoterAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-NT-PmNG
Plasmid#232200PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mNeonGreenDepositorInsertmNeongreen
ExpressionBacterialAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Human
Plasmid#238130PurposeThis plasmid encodes the full-length human TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.DepositorInsertTMEM106B (TMEM106B Human)
UseLentiviralTagsBlasticidin-P2A-3xHAExpressionMammalianPromoterCMVAvailable SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJCC_075 AsCas12a D908A
Plasmid#171668PurposeFor bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tagDepositorInsertAsCas12a D908A
Tags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged aspartate 908 to alanineAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJCC_076 AsCas12a R1226A
Plasmid#171669PurposeFor bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tagDepositorInsertAsCas12a R1226A
Tags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged arginine 1226 to alanineAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-eDHFR-Cdc42Q61L
Plasmid#107267PurposemCherry-eDHFR-Cdc42Q61L in pIVT vector for in vitro transcription. Note that Cdc42 in this plasmid keeps its CAAX domain.DepositorAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTrex-Luc-Phleo
Plasmid#48337PurposeTrasgenically expresses firefly luciferase in T. cruzi with phleomycin selectionDepositorInsertfirely luciferase
UseTrypanasoma cruzi expressionPromoterT. cruzi rRNAAvailable SinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mVenus-IQ6 WT
Plasmid#198201PurposeFRET acceptor: IQ6 domain of Moysin Va tagged with N-terminal mVenusDepositorAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ166-iSpyMac
Plasmid#149553PurposeGateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMsDepositorInsertiSpyMacCas9
UseCRISPR; Gateway compatible zispymaccas9 entry clo…Tags3X FLAG, NLS and NLSExpressionPlantMutationR221K, N394K, Protospacer interacting domain form…Available SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-nAChr-Alpha6
Plasmid#48812Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorAvailable SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ERBB4 (R544W)
Plasmid#29531DepositorAvailable SinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-M2-TLR2-P681H
Plasmid#12290DepositorInsertTLR2 (Tlr2 Mouse)
TagsFLAG (FLAG-M2)ExpressionMammalianMutationA74V, M82I, H202Y and P681H; signal peptide (aa1-…Available SinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
RGG-BcLOV-mCh-EGFR
Plasmid#208280PurposeExpresses RGG-BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.DepositorInsertRGG-BcLOV-mCh-EGFR
UseLentiviralMutationH225N in mCherry- please see depositor commentPromoterpCMVAvailable SinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMOX-mNG::LacI-V3
Plasmid#210224PurposeIntegrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.DepositorInsertmNeongreen Lac repressor fusion protein
UseTrypanosoma brucei expressionPromoterPol II readthrough transcription after integrationAvailable SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSETb MGlucoMeter2.6-1m
Plasmid#247471PurposeMatryoshka-based Glucose SensorDepositorInsertGlucose Binding Protein (GBP) with Matryoshka dual fluorophore cassette
ExpressionBacterialAvailable SinceFeb. 13, 2026AvailabilityAcademic Institutions and Nonprofits only -
pATZ-BFP-HA-GFP11
Plasmid#177721PurposeER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to blue fluorescence protein (BFP) tag protein, an HA tag and the eleventh β-strand of GFPDepositorInsertalpha-1-antitrypsin (SERPINA1 Human)
TagsGFP11, HA, and TagBFPExpressionBacterial and MammalianMutationE342K (ATZ mutant)Available SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEXndoS(E235Q)
Plasmid#44656DepositorInsertndoS (ndoS Streptococcus pyogenes)
TagsGstExpressionBacterialMutationChanged Glutamic acid 235 to GlutaminePromotertacAvailable SinceMay 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMMS22L-siR3
Plasmid#221013Purposemammalian expression vector of myc-Flag tagged MMS22L siRNA resistantDepositorInsertMMS22L (MMS22L Human)
TagsFlag and mycExpressionMammalianMutationsilent mutations MMS22L: 5'-aaAacGtgTtgCtgTg…PromoterCMVAvailable SinceJuly 31, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBad-CA TAG20
Plasmid#105666Purposeexpresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 20 and C-terminal 6x his tagDepositorArticleInserthuman carbonic anhydrase II thermostable variant (CA2 Human)
Tags6x his tagExpressionBacterialMutationTAG stop codon at site 20PromoterpBad/arabinoseAvailable SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only