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promoter J23110 (upstream of RBS, mRFP1)

Plasmid

#117852

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Publication

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

medium-high promoter, RBS, mRFP1

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM60 FLAG NUFIP1 W40A

Plasmid

#110951

Purpose

expresses mammalian FLAG-NUFIP1 W40A

Depositor

David Sabatini

Publication

Wyant et al Science. 2018 May 18;360(6390):751-758.

Insert

NUFIP1 W40A (NUFIP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed Tryptophan 40 to alanine

Promoter

CMV

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::NeoR-bGHpA

Plasmid

#62438

Purpose

MXS_chaining vector with PGK::NeoR-bGHpA

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Neomycin with PGK Promoter

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pMX-mTAF4b

Plasmid

#44345

Depositor

Marc Timmers

Publication

Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

Insert

TAF4b (Taf4b Mouse)

Use

Retroviral

Tags

flag and SBP

Expression

Mammalian

Promoter

LTR

Available since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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CALCOCO1 W47A-mycDDK

Plasmid

#137769

Purpose

CALCOCO1 LIR mutant

Depositor

Carol Mercer

Publication

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

CALCOCO1 W47A

Tags

mycDDK

Expression

Mammalian

Mutation

W47A

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pRPa-iSL(MCS1+2)

Plasmid

#69244

Purpose

Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.

Depositor

David Horn

Publication

Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7

Type

Empty backbone

Use

Expression in trypanosoma brucei

Promoter

RRNA promoter

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRExT2A-NT-PmNG

Plasmid

#232200

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mNeonGreen

Depositor

Calvin Tiengwe

Publication

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

mNeongreen

Expression

Bacterial

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Human

Plasmid

#238130

Purpose

This plasmid encodes the full-length human TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.

Depositor

Dirk Daelemans

Publication

Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

Insert

TMEM106B (TMEM106B Human)

Use

Lentiviral

Tags

Blasticidin-P2A-3xHA

Expression

Mammalian

Promoter

CMV

Available since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pJCC_075 AsCas12a D908A

Plasmid

#171668

Purpose

For bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Publication

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

AsCas12a D908A

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed aspartate 908 to alanine

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJCC_076 AsCas12a R1226A

Plasmid

#171669

Purpose

For bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Publication

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

AsCas12a R1226A

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed arginine 1226 to alanine

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-eDHFR-Cdc42Q61L

Plasmid

#107267

Purpose

mCherry-eDHFR-Cdc42Q61L in pIVT vector for in vitro transcription. Note that Cdc42 in this plasmid keeps its CAAX domain.

Depositor

Michael Lampson

Publication

Akera et al Science. 2017 Nov 3;358(6363):668-672. d

Insert

mCherry-eDHFR-Cdc42Q61L (CDC42, dhfr Human)

Tags

mCherry

Expression

Mammalian

Promoter

T7

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pTrex-Luc-Phleo

Plasmid

#48337

Purpose

Trasgenically expresses firefly luciferase in T. cruzi with phleomycin selection

Depositor

Rick Tarleton

Publication

Canavaci et al PLoS Negl Trop Dis. 2010 Jul 13;4(7):e74

Insert

firely luciferase

Use

Trypanasoma cruzi expression

Promoter

T. cruzi rRNA

Available since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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mVenus-IQ6 WT

Plasmid

#198201

Purpose

FRET acceptor: IQ6 domain of Moysin Va tagged with N-terminal mVenus

Depositor

Christian Wahl-Schott

Publication

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

MYO5A IQ domain (Myo5a Mouse)

Tags

mVenus

Expression

Mammalian

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ166-iSpyMac

Plasmid

#149553

Purpose

Gateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMs

Depositor

Yiping Qi

Publication

Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

Insert

iSpyMacCas9

Use

CRISPR; Gateway compatible zispymaccas9 entry clo…

Tags

3X FLAG, NLS and NLS

Expression

Plant

Mutation

R221K, N394K, Protospacer interacting domain form…

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-nAChr-Alpha6

Plasmid

#48812

Purpose

this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

Depositor

Henry Lester

Publication

Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

Insert

nAChr-alpha6 (Chrna6 Mouse)

Expression

Mammalian

Promoter

CMV

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ERBB4 (R544W)

Plasmid

#29531

Depositor

Yardena Samuels

Publication

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Expression

Mammalian

Mutation

changed arginine 544 to tryptophan

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-M2-TLR2-P681H

Plasmid

#12290

Depositor

Bruce Beutler

Publication

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

A74V, M82I, H202Y and P681H; signal peptide (aa1-…

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
RGG-BcLOV-mCh-EGFR

Plasmid

#208280

Purpose

Expresses RGG-BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Publication

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

RGG-BcLOV-mCh-EGFR

Use

Lentiviral

Mutation

H225N in mCherry- please see depositor comment

Promoter

pCMV

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSMOX-mNG::LacI-V3

Plasmid

#210224

Purpose

Integrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.

Depositor

Calvin Tiengwe

Publication

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Insert

mNeongreen Lac repressor fusion protein

Use

Trypanosoma brucei expression

Promoter

Pol II readthrough transcription after integration

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRSETb MGlucoMeter2.6-1m

Plasmid

#247471

Purpose

Matryoshka-based Glucose Sensor

Depositor

Wolf Frommer

Publication

Ishikawa et al bioRxiv 2025.09.27.678933; doi: 10.1101/

Insert

Glucose Binding Protein (GBP) with Matryoshka dual fluorophore cassette

Expression

Bacterial

Available since

Feb. 13, 2026

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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