Addgene

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C125-E01: stuffer

Plasmid

#162900

Purpose

Standard Gateway entry clone containing a non-transcribed/translated region for use in generating negative control vectors

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

stuffer fragment for vector controls

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCZGY3163

Plasmid

#101248

Purpose

GFP11::rps-18, ribosomal protein subunit, bound to C-terminal part of GFP. Shows GFP localized to ribosomes when combined with GFP 1-10.

Depositor

Yishi Jin

Article

Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

Insert

gfp11::rps-18 (rps-18 Nematode, Synthetic)

Use

Gateway

Tags

GFP11

Expression

Mutation

Promoter

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pME-Fv2E-flt4um200-ns

Plasmid

#67696

Purpose

middle entry plasmid with dimerizable extracellular domain fused to um200 mutant Danio Flt4 cytoplasmic domain

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

Fv2E-flt4um200

Use

Tags

HA

Expression

Mutation

um200, no stop codon; N1056S

Promoter

Available since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pAd PdxI-I-nGFP

Plasmid

#19411

Depositor

Douglas Melton

Article

Zhou et al Nature. 2008 Aug 27. ():.

Insert

pancreatic and duodenal homeobox 1 (Pdx1 Mouse)

Use

Adenoviral; Entry vector

Tags

GFP and IRES

Expression

Mutation

Promoter

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only
pYPQ154

Plasmid

#69305

Purpose

Gateway entry vector with AteCas9 (Arabidopsis codon optimized)

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

AteCas9 (Arabidopsis codon optimized)

Use

CRISPR

Tags

Expression

Mutation

NLS fusion

Promoter

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pEO100c

Plasmid

#50550

Purpose

Expresses sfGFP under the PompC1157 promoter

Depositor

Jeffrey Tabor

Article

Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

Insert

sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 22, 2014

Availability

Academic Institutions and Nonprofits only
p221z-dCAS9p-t35s

Plasmid

#118387

Purpose

Entry clone containing dCAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

dCAS9p

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pME GFP-PCNA

Plasmid

#105977

Purpose

entry clone for gateway cloning, cell cycle marker proliferative cell nuclear antigen PCNA

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

PCNA (PCNA Human)

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA1

Plasmid

#199727

Purpose

Golden Gate entry vector; 1st gRNA under OsU3 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

OsU3-sgRNA1 scaffold

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA2

Plasmid

#199728

Purpose

Golden Gate entry vector; 2nd gRNA under OsU3 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

OsU3-sgRNA2 scaffold

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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