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LLP340 pEF1a-NLS-scFvGCN4-DNMT3a (mutant)

Plasmid

#100944

Purpose

Single chain variable fragment antibody GCN4 fused to DNMT3a catalytic mutant, binds SunTag, with 3xTy1 tag

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

scFvGCN4, sfGFP, GB1, DNMT3a catalytic mutant (DNMT3A Synthetic, Human)

Tags

3xTy1

Expression

Mammalian

Mutation

F636A, E660A, E725A, R295A

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pSc1-DD

Plasmid

#80439

Purpose

Expresses eGFP with ecDHFR along with an U6 promoter driven gRNA which cleaves the plasmid in-cell

Depositor

Ervin Welker

Article

Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

Inserts

sp Cas9 gRNA
eGFP

Use

CRISPR

Tags

E. coli dihydrofolate reductase

Expression

Mammalian

Promoter

CMV and U6

Available Since

Aug. 11, 2016

Availability

Academic Institutions and Nonprofits only

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LMPd Ametrine Got2 shRNA#3

Plasmid

#220593

Purpose

Retroviral vector with Ametrine marker for expression shRNA with an "UltramiR" microRNA scaffold

Depositor

Russell Jones

Article

Ma et al bioRxiv [Preprint]. 2023 Jun 11:2023.06.

Insert

Got2 shRNA (Got2 Mouse)

Use

Retroviral

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-EE-UdgX-NG-nCas9-UdgX

Plasmid

#163572

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-EE-UdgX-NG-nCas9-UdgX

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pNTKV- Elf5-KI-3'UTR-2A-EYFP-NLS

Plasmid

#128833

Purpose

Knock-in of nuclear EYFP into mouse Elf5 locus

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

Nuclear EYFP into Elf5 locus (Elf5 Mouse)

Use

Mouse Targeting

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PPO-Venus

Plasmid

#139502

Purpose

Expresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Insert

parapinopsin

Tags

Venus tag following 3x Ala linker

Expression

Mammalian

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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VARP GFP-pLXIN

Plasmid

#62950

Purpose

full length human VARP with C-terminal EGFP tag cloned into pLXIN

Depositor

Paul Luzio

Article

Hesketh et al Dev Cell. 2014 Jun 9;29(5):591-606. doi:

Insert

VARP (ANKRD27 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

silent mutations of amino acids 422-428 to produc…

Promoter

LTR

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CoV2-Omicron BA.1 spike

Plasmid

#194602

Purpose

Mammalian expression of RBD domain from SARS-CoV-2 omicron (BA.1)

Depositor

Ben Murrell

Article

Sheward et al Lancet Infect Dis. 2022 Jun;22(6):813-82

Insert

CoV2-Omicron BA.1 spike (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SHP-2(N)-SH2

Plasmid

#46498

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHP-2(N) (PTPN11 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-104

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk1a1MKK7B2

Plasmid

#19731

Depositor

Roger Davis

Article

Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

Insert

Jnk1a1MKK7b2 (Map2k7, MAPK8 Human, Mouse)

Tags

Flag

Expression

Mammalian

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-mScarlet-gamma9

Plasmid

#140097

Purpose

Expresses an mScarlet-tagged gamma9 subunit for visualizing G beta-gamma translocation

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Insert

GNGT2

Tags

N-terminal mScarlet tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX Abl-SH2

Plasmid

#46402

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Abl (ABL1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 120-225

Promoter

Tac

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP C1-Eps8 ∆cap

Plasmid

#74786

Purpose

mammalian expression of the capping activity mutant Eps8 fused to GFP

Depositor

Giorgio Scita

Article

Hertzog et al PLoS Biol. 2010 Jun 1;8(6):e1000387. doi

Insert

Eps8 (Eps8 Mouse)

Expression

Mammalian

Mutation

V689D, L693D

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-CMV-CBA-hGBA1-cm-hGBA1-hGHpA

Plasmid

#218795

Purpose

AAV reporter genome plasmid

Depositor

Ben Deverman

Article

Huang et al Science. 2024 Jun 14;384(6701):1220-1227

Insert

CMV-CBA-hGBA1-cm-hGBA1-hGHpA

Use

AAV

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCSCRET2(#323)

Plasmid

#184058

Purpose

cyclofen-inducible CRE activation via mammalian cell transfection or mRNA synthesis

Depositor

Sophie Vriz

Article

Sinha et al Zebrafish. 2010 Jun;7(2):199-204. doi: 1

Insert

6myc-CRE-ERT2

Use

Synthetic Biology

Tags

6xMyc

Expression

Mammalian

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

sCMV+SP6

Available Since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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R620-M15-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 D614G

Plasmid

#170201

Purpose

Mammalian expression of SARS-CoV-2 soluble spike trimer protein (D614G mutant)

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

SARS-CoV-2 S (spike) D614G (S SARS-CoV-2)

Tags

His6

Expression

Mammalian

Mutation

D614G

Promoter

CMV51

Available Since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

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3322_pETcon_SARS2_Omicron-BA2

Plasmid

#184409

Purpose

yeast surface display of the SARS-CoV-2 Omicron BA.2 variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Omicron BA.2 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

G339D+S371F+S373P+S375F+T376A+D405N+R408S+K417N+N…

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3_ATP10B

Plasmid

#203695

Purpose

expresses ATP10B

Depositor

Peter Vangheluwe

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Expression

Mammalian

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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TRCN0000072225

Plasmid

#78160

Purpose

shLacZ control

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

LacZ

Use

Lentiviral and RNAi

Promoter

hU6 and hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pQCXIP mScarlet-MOSPD2 W201E

Plasmid

#186477

Purpose

Expression of human MOSPD2 (mutant W201E, unable to bind lipid droplets) fused to mScarlet in mammalian cells

Depositor

Catherine Tomasetto

Article

Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Use

Retroviral

Tags

mScarlet

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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