We narrowed to 16,981 results for: tes

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pADSS-EGFP

Plasmid

#228197

Purpose

expressed human ADSS with an EGFP fluorescent protein tag

Depositor

Stephen Benkovic

Article

Zhao et al J Biol Chem. 2015 Mar 13;290(11):6705-13

Insert

ADSS (ADSS1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNK115

Plasmid

#219745

Purpose

MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Nicotiana benthamiana

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

mutant of fungal luciferase

Use

Luciferase and Synthetic Biology

Mutation

I3S, N4T, F11L, I63T, T99P, T192S, A199P

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-AGG-SpCas9

Plasmid

#214603

Purpose

encode SpCas9; in vitro transcribed modified RNA vector

Depositor

Yuxuan Guo

Article

Huang et al Int Immunopharmacol. 2024 Oct 17;143(Pt

Insert

SpCas9

Use

CRISPR and Unspecified

Promoter

T7

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-SPARCL1-N-HATag

Plasmid

#218185

Purpose

To tag Hevin with HA at the N-terminal

Depositor

Cagla Eroglu

Article

Bindu et al eLife. 2024, Reviewed Preprint. 13:RP103

Insert

sgRNA (Sparcl1 Mouse)

Use

AAV and CRISPR

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-Vamp2-GeneTRAP

Plasmid

#218187

Purpose

To KO and genetrap mouse Vamp2 simultaneously

Depositor

Cagla Eroglu

Article

Bindu et al eLife. 2024, Reviewed Preprint. 13:RP103

Insert

sgRNA (Vamp2 Mouse)

Use

AAV and CRISPR

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-SPARCL1-C-mCherryTag

Plasmid

#218183

Purpose

To tag Hevin with mCherry at its C-terminal

Depositor

Cagla Eroglu

Article

Bindu et al eLife. 2024, Reviewed Preprint. 13:RP103

Insert

sgRNA (Sparcl1 Mouse)

Use

AAV and CRISPR

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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CMV-dCas9_puroR

Plasmid

#219825

Purpose

CMV-driven expression of dCas9 with puromycin resistance (Adapted from plasmid #68416)

Depositor

Philipp Maass

Insert

dCas9

Use

CRISPR

Expression

Mammalian

Mutation

D10A/H841A

Promoter

CMV

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-BI-hTFR1-4

Plasmid

#218799

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Article

Huang et al Science. 2024 Jun 14;384(6701):1220-1227

Insert

AAV Rep-Cap BI-hTFR1-4

Expression

Mammalian

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pZG71

Plasmid

#202577

Purpose

RNA editing in plants. Plasmid contains 35S-dADARcd-LIC1-ccdB-LIC2-dADARcd-35S-hptII

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Expression

Plant

Mutation

E488Q

Available Since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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G1095C_BattB(GA)_jGCaMP7c_IRES_shBleR-P2A-HygroR

Plasmid

#200638

Purpose

jGCaMP7c recombination plasmid for Matreyek Bxb1(GA) landing pad in dual landing pad cells

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

jGCaMP7c

Expression

Mammalian

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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PB-Blast-Dest-LifeAct-mRuby

Plasmid

#194907

Purpose

To generate LifeAct-mRuby expressing stable cell lines with piggybac transposase co-expression and blastocidin selections.

Depositor

Clare Buckley

Article

Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

Insert

LifeAct

Use

Mouse Targeting

Tags

mRuby

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-Camk2a

Plasmid

#186818

Purpose

Fluorescent reporter for Camk2a expression

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

Camk2a promoter (Camk2a Mouse)

Use

Luciferase

Promoter

Camk2a promoter

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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V5-W279A-short-UBASH3A

Plasmid

#192086

Purpose

Mammalian expression of human W279A short-form UBASH3A with V5 tag

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Tags

V5

Expression

Mammalian

Mutation

W279A

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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PALI1_pHIVEGFP

Plasmid

#191252

Purpose

Expresses PALI1 in mammalian cells

Depositor

Chen Davidovich

Article

Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

Insert

LCoR

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

NA

Promoter

EF1alpha

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1_Orange

Plasmid

#160444

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Orange chromoprotein in E. coli

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Orange

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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ApoE3_mCh-SspB (pBS1144)

Plasmid

#185326

Purpose

For the mammalian expression of the human protein ApoE3 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE3

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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ApoE2_mCh-SspB (pBS1142)

Plasmid

#185324

Purpose

For the mammalian expression of the human protein ApoE2 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE2

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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AfrLEA3m_mCh-2xFKBP (pBS1137)

Plasmid

#185321

Purpose

For the mammalian expression of the brine shrimp protein AfrLEA3m attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

AfrLEA3m

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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p416-GPD-HA-PPK

Plasmid

#183943

Purpose

Expresses HA-tagged E. coli PPK from yeast GPD promoter

Depositor

Michael Downey

Article

McCarthy et al mBio. 2022 Aug 30;13(4):e0039022. doi: 1

Insert

PPK (ppk E. coli)

Tags

HA

Expression

Yeast

Mutation

WT

Promoter

GPD

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_KPNA4_Arm

Plasmid

#178495

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

KPNA4_Arm (KPNA4 Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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