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pAAV-EFS-hfCas13d-P2A-mCherry-pA

Plasmid

#233026

Purpose

To Express HA Tagged hfCas13d and mcherry from the mammalian EFS promoter. The hfCas13d and mCherry are separated by a P2A site

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

hfCas13d/mCherry

Use

AAV

Tags

mCherry

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-MBP-TEV-NAP1_delta-NDP52 (S37K/A44E)

Plasmid

#208872

Purpose

For expression in E. coli of NAP1 (delta-NDP52)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

MBP-TEV

Expression

Bacterial

Mutation

S37K/A44E (disrupts NDP52 binding)

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-MBP-TEV-NAP1_delta-TBK1 (L226Q/L233Q)

Plasmid

#208873

Purpose

For expression in E. coli of NAP1 (delta-TBK1)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

MBP-TEV

Expression

Bacterial

Mutation

L226Q/L233Q (disrupts TBK1 binding)

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-GFP-NAP1-S37K/A44E (delta-NDP52)

Plasmid

#208863

Purpose

Stably express GFP-tagged NAP1 (delta-NDP52) for CID induced mitophagy

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

FKBP-GFP

Expression

Mammalian

Mutation

S37K/A44E (disrupts NDP52 binding)

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-GFP-NAP1-I11S/L12S (delta-FIP200)

Plasmid

#208864

Purpose

Stably express GFP-tagged NAP1 (delta-FIP200) for CID induced mitophagy

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

FKBP-GFP

Expression

Mammalian

Mutation

I11S/L12S (disrupts FIP200 binding)

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-GFP-NAP1-L226Q/L233Q (delta-TBK1)

Plasmid

#208865

Purpose

Stably express GFP-tagged NAP1 (delta-TBK1) for CID induced mitophagy

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

FKBP-GFP

Expression

Mammalian

Mutation

L226Q/L233Q (disrupts TBK1 binding)

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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CSII-PPM1H-mApple-mito

Plasmid

#207351

Purpose

Mammalian expression of PPm1H-mito

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPm1H-mito (PPM1H Human)

Expression

Mammalian

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-PPM1H S124A

Plasmid

#208368

Purpose

Mammalian expression of HA-tagged human PPM1H S124A

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

HA-PPM1H S124A (PPM1H Human)

Expression

Mammalian

Mutation

S124A

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-PPM1H S211A

Plasmid

#208369

Purpose

Mammalian expression of HA-tagged human PPM1H S211A

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

HA-PPM1H S211A (PPM1H Human)

Expression

Mammalian

Mutation

S211A

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ10 PPM1H

Plasmid

#207335

Purpose

Mammalian expression of HA-Δ10 PPM1H

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ10

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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