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pDonr 201_USP7_C223S

Plasmid

#240320

Purpose

Entry vector for Gateway with USP8 (C223A)

Depositor

Junjie Chen

Article

Nie et al Genes Dev. 2022 Sep 1;36(17-18):1016-103

Insert

USP7 (USP7 Human)

Expression

Bacterial

Mutation

C223A

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pET24+ CUL5(1-384)

Plasmid

#246504

Purpose

Recombinant protein expression of CUL5(1-384)

Depositor

Steven Clarke

Article

Pang et al bioRxiv [Preprint]. 2025 May 21:2025.05.

Insert

cullin-5 (CUL5 Human)

Expression

Bacterial

Mutation

Deleted amino acids 385-780

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-AHR-shRNA1

Plasmid

#166889

Purpose

Lentivirus for inducible knockdown of human AHR

Depositor

Roland Friedel

Article

Halawani et al bioRxiv 2023.11.04.565649. doi: https://

Insert

AHR shRNA (AHR Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pZNpF-∆48∆11

Plasmid

#245369

Purpose

Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-∆48∆11 (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Mutation

11-bp deletion, H96Wfs*4, plus synthetic 48-bp up…

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pVBL0001

Plasmid

#242429

Purpose

Stable genomic integration of Opto-IRE1 (HsIRE1dLD-mCherry-CRY2clust) using the Flp-in system.

Depositor

Vladislav Belyy

Article

Smith et al bioRxiv [Preprint]. 2025 Jul 21:2025.07.

Insert

IRE1alpha (ERN1 Human)

Tags

mCherry, CRY2clust

Expression

Mammalian

Mutation

Deletion of the lumenal domain

Promoter

CMV, tet-inducible

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA-d5-HT1AR

Plasmid

#221820

Purpose

Plasmid to express gRNA (gaccagtccactaccgcagc) for editing at the end of Drosophila 5-HT1AR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

d5-HT1AR gRNA (5-HT1A Fly)

Use

CRISPR

Expression

Insect

Promoter

zebrafish U6 promoter (U6b)

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA1-d5-HT1BR

Plasmid

#221821

Purpose

Plasmid to express gRNA1 (gaaaatttgatttcaactga) for editing at the end of Drosophila 5-HT1BR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

d5-HT1BR gRNA1 (5-HT1B Fly)

Use

CRISPR

Expression

Insect

Promoter

zebrafish U6 promoter (U6b)

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA2-5-HT1BR

Plasmid

#221822

Purpose

Plasmid to express gRNA2 (aatttcgacgggccttcaag) for editing at the end of Drosophila 5-HT1BR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

d5-HT1BR gRNA2 (5-HT1B Fly)

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6 promoter

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA1-5-HT2AR-isoformD

Plasmid

#221823

Purpose

Plasmid to express gRNA1 (tccttctggcgcaaacacgg) for editing at the end of Drosophila 5-HT2AR isoform D coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

d5-HT2AR isoform D gRNA1 (5-HT2 Fly)

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6 promoter

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA2-5-HT2AR-isoformD

Plasmid

#221824

Purpose

Plasmid to express gRNA2 (ctgaagacataattacgtgg) for editing at the end of Drosophila 5-HT2AR isoform D coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

d5-HT2AR isoform D gRNA2 (5-HT2 Fly)

Use

CRISPR

Expression

Insect

Promoter

zebrafish U6 promoter (U6b)

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA1-5-HT2AR-isoformBFH

Plasmid

#221825

Purpose

Plasmid to express gRNA1 (cgctatcggtctgtgacaga) for editing at the end of Drosophila 5-HT2AR isoforms B, F and H coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

d5-HT2AR isoforms BFH gRNA1 (5-HT2 Fly)

Use

CRISPR

Expression

Insect

Promoter

zebrafish U6 promoter (U6b)

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA2-5-HT2AR-isoformBFH

Plasmid

#221826

Purpose

Plasmid to express gRNA2 (ggaaaagccgctaattacag) for editing at the end of Drosophila 5-HT2AR isoforms B, F and H coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

d5-HT2AR isoforms BFH gRNA2 (5-HT2 Fly)

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6 promoter

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA-d5-HT7R

Plasmid

#221829

Purpose

Plasmid to express gRNA (ggcgagggagagctttctct) for editing at the end of Drosophila 5-HT1AR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

d5-HT7R gRNA (5-HT7 Fly)

Use

CRISPR

Expression

Insect

Promoter

zebrafish U6 promoter (U6b)

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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M7138

Plasmid

#219774

Purpose

MoClo-compatible Level M vector encoding hygromycin resistance cassette and a luciferase gene under control of ORCA3 promoter and terminator

Depositor

Karen Sarkisyan

Article

Balakireva et al bioRxiv 2025.02.17.636591; doi: https://

Insert

pNOS-HygR-ocsT | pORCA3-nnLuz-ORCA3_T

Use

Synthetic Biology

Expression

Plant

Promoter

pNOS-HygR-ocsT | pORCA3-nnLuz-ORCA3_T

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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M7691

Plasmid

#219775

Purpose

MoClo-compatible Level M vector encoding hygromycin resistance cassette and a luciferase gene under control of WRKY70 promoter and terminator

Depositor

Karen Sarkisyan

Article

Balakireva et al bioRxiv 2025.02.17.636591; doi: https://

Insert

pNOS-HygR-ocsT | pWRKY70-nnLuz-WRKY70_T

Use

Synthetic Biology

Expression

Plant

Promoter

pNOS-HygR-ocsT | pWRKY70-nnLuz-WRKY70_T

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pNK3558

Plasmid

#219776

Purpose

MoClo-compatible Level M vector encoding hygromycin resistance cassette and a luciferase gene under control of p35S promoter and act2 terminator

Depositor

Karen Sarkisyan

Article

Balakireva et al bioRxiv 2025.02.17.636591; doi: https://

Insert

pNOS-HygR-ocsT | p35S-nnLuz-act2

Use

Synthetic Biology

Expression

Plant

Promoter

pNOS-HygR-ocsT | p35S-nnLuz-act2

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-sfGFP-TurboID-ER

Plasmid

#240230

Purpose

Expression of ER-localized sfGFP-TurboID under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

BIP-sfGFP-TurboID-KDEL

Expression

Insect

Promoter

MT

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-sfGFP-TurboID

Plasmid

#240231

Purpose

Expression of sfGFP-TurboID under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

sfGFP-TurboID

Expression

Insect

Promoter

MT

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-Myc-BirA*G3-ER

Plasmid

#240234

Purpose

Expression of ER-localized Myc-BirA*-G3 under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

BiP-Myc-BirA*G3-KDEL

Expression

Insect

Promoter

MT

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pWal10-roe-sfGFP

Plasmid

#240239

Purpose

Gateway destination plasmid to generate C-terminal sfGFP fusions under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Type

Empty backbone

Use

Gateway destination vector

Expression

Insect

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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