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Seattle_IPDA_control_29_006

Plasmid

#167352

Purpose

ddPCR gating control for droplets containing either env+5'pol targets OR env+3'pol targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol_env

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCRI006-pYFAC-riboB-PgpdA-dSpCas9-VPR-TtrpC

Plasmid

#140199

Purpose

Episomal expression of dSpCas9-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Insert

dSpCas9-VPR

Use

CRISPR and Synthetic Biology; Expression in asper…

Tags

VPR (VP64-p65-Rta), NLS

Expression

Mutation

Promoter

PgpdA

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCRI004-pYFAC-riboB-PgpdA-dLbCas12a-VPR-TtrpC

Plasmid

#140197

Purpose

Episomal expression of dLbCas12a-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Insert

dLbCas12a-VPR

Use

CRISPR and Synthetic Biology; Expression in asper…

Tags

VPR (VP64-p65-Rta), NLS

Expression

Mutation

D832A DNase deactivated

Promoter

PgpdA

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS_HA-VP35_EBOV

Plasmid

#103053

Purpose

Mammalian expression vector encoding HA-tagged Ebola virus VP35 gene under control of the CAG promoter

Depositor

Elke Mühlberger

Article

Nelson et al J Virol. 2016 Jul 27;90(16):7268-84. doi

Insert

VP35 (VP35 Zaire ebolavirus, Mayinga)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pEMS2113

Plasmid

#49138

Purpose

AAV plasmid with FEV (Ple67) promoter driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

ssAAV-Ple67-emGFP-WPRE

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

FEV

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pT2-TetR-neoR

Plasmid

#153310

Purpose

Tet-repressor with neomycin selection marker, to be used together with pT2-CMV/TetO2-EYFP-GW

Depositor

Spyros Petrakis

Article

Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

Insert

TetR

Use

Transposon

Tags

Expression

Mutation

Promoter

SV40

Available since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-hLC3

Plasmid

#87872

Purpose

Express a EGFP-human MAP1-LC3 in mammalian cells

Depositor

Isei Tanida, Eiki Kominami

Article

Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

Insert

MAP1LC3 (MAP1LC3B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-NRASQ61K-IRES-Puro

Plasmid

#120570

Purpose

Expresses Flag-HA-6xHIS-N-Ras Q61K fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Q61K

Promoter

CMV

Available since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pCX0393

Plasmid

#205984

Purpose

Ga0116245_10002768 T7/LacO-Cas6 + Cas14-TwinStrep + Cas7 + His14-Cas5::pJ23119-truncated DR-truncated Fn spacer

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Inserts

Ga0116245_10002768 candidate type VII proteins
Cas14
Cas5
truncated CRISPR array

Use

Tags

14xHis and TwinStrep-tag

Expression

Bacterial

Mutation

Promoter

T7 (proteins), pJ23119 (crRNA)

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pEMS1991

Plasmid

#49122

Purpose

AAV plasmid with FEV (Ple67) promoter driving expression of iCre. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

ssAAV-Ple67-iCre-WPRE

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

FEV

Available since

May 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pLEX-FHH-NRAS-IRES-Puro

Plasmid

#120569

Purpose

Expresses Flag-HA-6xHIS-N-Ras wildtype fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Puro-EF-1α>{H2B-RFP}

Plasmid

#201121

Purpose

RFP expression in cells under EF-1α promoter; cytoplasmic and histone-bound, nuclear

Depositor

Ori Bar-Nur

Article

Zvick et al Stem Cell Reports. 2022 Sep 13;17(9):194

Insert

H2B-RFP

Use

Lentiviral

Tags

Expression

Mutation

No

Promoter

Available since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pU6-mir144~451 EF1Alpha-puro-T2A-BFP

Plasmid

#164793

Purpose

Express miR-144~451 under the U6 promoter in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144~451 (MIR451A, MIR144 Human)
puro-T2A-BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1Alpha and U6

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX-FLAG-NRASQ61K-IRES-Puro

Plasmid

#120575

Purpose

Expresses Flag-N-Ras Q61K fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Q61K

Promoter

CMV

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCAP03-sp44/p21

Plasmid

#120232

Purpose

Carries bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites that can be amplified with homology sequence for refactoring.

Depositor

Bradley Moore

Article

Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

Insert

bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites

Use

Synthetic Biology

Tags

Expression

Mutation

aac(3)IV (apramycin resistance gene)

Promoter

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pEIGW-human KLF4

Plasmid

#100131

Purpose

Human KLF4 lentiviral expression vector

Depositor

Eva Hernando

Article

Segura et al Oncotarget. 2017 Apr 18;8(16):26298-2631

Insert

Kruppel like factor 4 (KLF4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

E1F

Available since

Feb. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLEX-uORF-HA-birA*-H-Ras(G12V)-IRES-Puro

Plasmid

#120560

Purpose

Expresses HA-birA*-H-Ras G12V mutant fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

HRAS (HRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Mutation

G12V

Promoter

CMV

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pT2-CMV/TetO2-EYFP-GW

Plasmid

#153309

Purpose

Tet-On YFP-tagged Gateway destination vector, to be used together with pT2-TetR-neoR transposon plasmid

Depositor

Spyros Petrakis

Article

Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

Type

Empty backbone

Use

Transposon

Tags

Yellow Fluorescent Protein

Expression

Mutation

Promoter

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX-FLAG-NRAS-IRES-Puro

Plasmid

#120574

Purpose

Expresses Flag-N-Ras wildtype fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-hGATE-16

Plasmid

#87870

Purpose

Express a EGFP-human GATE-16 in mammalian cells

Depositor

Isei Tanida, Eiki Kominami

Article

Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

Insert

GATE-16 (GABARAPL2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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