We narrowed to 3,597 results for: Gla

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pEB2_mScarletI_mVenusNB-MrkH

Plasmid

#182291

Purpose

expresses c-di-GMP biosensor in E. coli

Depositor

Howard Berg

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2022 Feb 8;119

Insert

mVenusNB-MrkH

Tags

mVenusNB internal fusion to MrkH

Expression

Bacterial

Promoter

proC

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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(302) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5)

Plasmid

#168192

Purpose

HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGT of 4 TPRs

Depositor

Christina Woo

Article

Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

Insert

HA-LaG6-(EAAAK)4-OGT(4)

Tags

HA-Tag

Expression

Mammalian

Promoter

T7/CMV

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAJM.969

Plasmid

#108537

Purpose

MphRAM + EryR + PMph-YFP Reporter. Ery sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

MphRAM + EryR + PMph-YFP

Expression

Bacterial

Mutation

MphR: N94D

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GoldenGate-VP64

Plasmid

#47389

Purpose

Expression plasmid for TALE proteins fused to VP64 activation domain compatible with GoldenGate cloning system

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nat Methods. 2013 Mar;10(3):239-42. doi:

Type

Empty backbone

Use

TALEN

Tags

FLAG, HA, and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pXD70LacZ2-Pdadh-LacZ

Plasmid

#191618

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), Pdadh dopamine dehydroxylase promoter-lacZ

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET21b-LaG9(S151C)

Plasmid

#172490

Purpose

Encodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).

Depositor

Siegfried Musser

Article

Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

Insert

LaG9(S151C)

Tags

6xHis, PPX cleavage site, and pelB leader sequenc…

Expression

Bacterial

Mutation

S151C

Promoter

T7

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pPBpuro-COX2-EBFP2

Plasmid

#184720

Purpose

Exogenous COX2

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

COX2-EBFP2 (PTGS2 Synthetic, Human)

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pUB-Cas9-@GL1

Plasmid

#86779

Purpose

Disruption of GLABROUS1 gene in Arabidopsis using CRISPR/Cas9

Depositor

Andreas Weber

Article

Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

Insert

sgRNA against GLABROUS1

Use

Synthetic Biology

Expression

Plant

Promoter

Arabidopsis U6-26 promoter

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pL2-GGA(EAAAK)2AGG

Plasmid

#137104

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

GGA(EAAAK)2AGG

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pYC34

Plasmid

#63915

Purpose

Recipient overexpressing vector to construct mCherry tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

mCherry

Expression

Yeast

Promoter

TEF1 (Saccharomyces cerevisiae)

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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Human USP30 (64-502, construct 13, MG-26-82)

Plasmid

#110746

Purpose

Bacterial expression of human USP30 (construct 13)

Depositor

David Komander

Article

Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

Insert

USP30 (USP30 Human)

Tags

N-His6-3C-GST-3C

Expression

Bacterial

Mutation

F348D, M350S, I353E + insertion deletion: 64-178;…

Promoter

T7

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pXD70Tet(DadR)-DadR

Plasmid

#191636

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR complementation

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

DadR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSW002-PpsbA

Plasmid

#108236

Purpose

Broad host bacterial expression vector with constitutive PsbA promoter

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

PsbA promoter

Expression

Bacterial

Available Since

May 7, 2018

Availability

Academic Institutions and Nonprofits only

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pYC121

Plasmid

#63917

Purpose

Recipient overexpressing vector to construct MYC tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

MYC

Expression

Yeast

Promoter

TEF1 (Saccharomyces cerevisiae)

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pYC119

Plasmid

#63916

Purpose

Recipient overexpressing vector to construct FLAG tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

FLAG

Expression

Yeast

Promoter

TEF1 (Saccharomyces cerevisiae)

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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(366) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4, K852A)

Plasmid

#168189

Purpose

HA-tagged Ubc6E nanobody fused to an inactive truncated OGT of 4 TPRs with K852A mutation

Depositor

Christina Woo

Article

Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

Insert

HA-nUbc6E-(EAAAK)4-OGT(4,K852A)

Tags

HA-Tag

Expression

Mammalian

Promoter

T7/CMV

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pJJF439_PU6_empty_sgRNAscaffold - sgRNA cloning backbone

Plasmid

#164266

Purpose

sgRNA cloning backbone for expression of sgRNA in C. elegans. Contains BbsI sites for insertion of spacer sequences. U6 promoter from W05B2.8 gene.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Type

Empty backbone

Use

CRISPR

Expression

Worm

Promoter

U6 promoter from W05B2.8

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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Pediculus humanus corporis PINK1 (143 - 575)

Plasmid

#110751

Purpose

Bacterial expression of PhPINK1 (crystallography)

Depositor

David Komander

Article

Schubert et al Nature. 2017 Dec 7;552(7683):51-56. doi:

Insert

Phum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)

Tags

N-His6-SUMO

Expression

Bacterial

Promoter

T7

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pXD70LacZ3-Pcsd-LacZ

Plasmid

#191619

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), Pcsd promoter-lacZ, E. lenta constitutive promoter

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pYC25

Plasmid

#63914

Purpose

Recipient overexpressing vector to construct YFP tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

YFP

Expression

Yeast

Promoter

TEF1 (Saccharomyces cerevisiae)

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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