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We narrowed to 107 results for: Gla

Showing: 1 - 20 of 107 results

  1. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...Depositor Collections New England Biolabs Cell-Imaging Plasmid Collection New England Biolabs Cell-Imaging ...New England Biolabs has deposited plasmids at Addgene to generate SNAP, CLIP, ACP or MCP tagged plasmids...Technology Plasmids Supplemental Information New England Biolabs offers non-toxic protein labeling systems...To learn more about this system, visit the New England Biolabs website. For a comprehensive comparison...Substrate source (PDF, 46 KB) Please note: New England Biolabs (NEB) has discontinued the ACP-tag/MCP-...

  2. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...GENIE, Douglas Kim AV-1-PV2817 100839-AAV1 pAAV.CAG.Flex.GCaMP6m.WPRE.SV40 Biosensor GENIE, Douglas Kim ...GENIE, Douglas Kim AV-1-PV2819 100833-AAV1 pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 Biosensor GENIE, Douglas Kim ...GENIE, Douglas Kim AV-1-PV2821 100845-AAV1 pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 Biosensor GENIE, Douglas Kim ...GENIE, Douglas Kim AV-1-PV2823 100841-AAV1 pAAV.Syn.GCaMP6m.WPRE.SV40 Biosensor GENIE, Douglas Kim AV-...GENIE, Douglas Kim AV-1-PV2833 100844-AAV1 pAAV.CAG.GCaMP6s.WPRE.SV40 Biosensor GENIE, Douglas Kim AV-...GENIE, Douglas Kim AV-1-PV3081 100836-AAV1 pAAV.CAG.GCaMP6f.WPRE.SV40 Biosensor GENIE, Douglas Kim AV-...GENIE, Douglas Kim AV-1-PV3846 100857-AAV1 pAAV.Syn.NES-jRGECO1b.WPRE.SV40 Biosensor GENIE, Douglas Kim ...

  3. DNA Purification Without a Kit

    Type
    Blog Post
    ...columns, it’s bound to glass syringe filters. Normally DNA does not bind silica or glass, but the addition ...coaxes it into sticking to the hydrophobic glass filter. Each glass filter can capture up to 150 ug of DNA...rather a glass powder slurry that has a milk-like appearance. To make DNA stick to the glass particles...and 2) it helps DNA stick to the glass. After binding DNA, the glass particles are pelleted by centrifugation...Plasmid purification and DNA gel extraction with glass syringe filters Starting material: bacterial cultures...binding capacity. Figure 1: Purify DNA using glass filters. Image: Kim and Morrison, 2009. This ...Morrison method, you can just adjust the number of glass filters you use depending on the size of bacterial...

  4. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...microcompartments. Nat Methods. 2019 Jul;16(7):649-657. Douglas Kim , GENIE Project Calcium GCaMP6f/m/s calcium...activity. Nature. 2013 Jul 18;499(7458):295-300. Douglas Kim , GENIE Project Calcium Bicistronic vector ...Neurosci. 2012 Oct 3;32(40):13819-40. Baljit Khakh , Douglas Kim , Loren Looger , GENIE Project Calcium GCaMP3...neural activity. eLife. 2016 Mar 24;5. pii: e12727. Douglas Kim , GENIE Project Calcium Red Calcium sensor ...

  5. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

    Type
    Blog Post
    ... Gladys Kaba from the University of Health and Allied Sciences in Ghana will be receiving the third Addgene...Duedu who were awarded similar grants back in June, Gladys will be using plasmids in creative ways that we...applications. Cervical cancer screening in Ghana Gladys is currently working to enhance cervical cancer...of high risk (HR) Human Papillomavirus (HPV). As Gladys wrote in her application, “Cervical Cancer is the...plasmids as positive controls To start her work, Gladys will improve cervical cancer screening for a limited...alongside the cytological tests currently in use. Gladys will use plasmids containing a variety of HPV genes...management. We are incredibly happy to hear that Gladys will be using plasmids in a way that can potentially...

  6. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...lab One stingy science project was to make my own glass milk for purifying cut plasmid DNA from agar slices...lifetime supply for about $10 US dollars worth of glass powder pruchased from a pottery supply house.  These... when noting that one step involves boiling the glass powder in 50% nitric acid, but I never let that ...that stop me. I only had to replenish my glass milk supplies every 10 years or so. Actually, I was happy ... smaller gel apparatus until I settled on using glass slides. With just the right amount of agarose, I... I could pour a very thin gel on a large glass slide using only surface tension.  It's a bit tricky to...

  7. Immunology Research Plasmids and Resources

    Type
    Collection
    ...PAFR PTGDR prostaglandin D2 receptor (DP) AS1, ASRT1, DP, DP1, MGC49004 PTGDS prostaglandin D2 synthase..., PGDS2 PTGER1 prostaglandin E receptor 1 (subtype EP1), 42kDa EP1 PTGER2 prostaglandin E receptor 2 (...MGC27302 PTGER4 prostaglandin E receptor 4 (subtype EP4) EP4, EP4R, MGC126583 PTGFR prostaglandin F receptor... (subtype EP2), 53kDa EP2 PTGER3 prostaglandin E receptor 3 (subtype EP3) EP3, EP3-I, EP3-II, EP3-III,...

  8. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...Research Symposium organizer, David T. Riglar PhD. Here, Dr. Riglar discusses one of the six panel discussions...Many thanks to our guest blogger David T. Riglar! David T Riglar is a postdoc at Harvard Medical School....science and public policy. Follow David on Twitter @driglar.     References 1. Alberts, Bruce, et al. "Rescuing...

  9. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    ...in the past year. Cambridge Biological Society, England Since 1996, Cambridge Biosoc’s goal has been to... up to professors. Oxford Biological Society, England The Biological Society based at the University ...networking events. Imperial Synthetic Biology Society, England The Synthetic Biology Society is a new society ...Inspiring future scientists At Addgene, we’re incredibly glad to see these biological societies engaging with ...

  10. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...by New England Biolabs, Inc. Many thanks to our guest blogger Lydia Morrison from New England Biolabs...contributed by guest blogger Lydia Morrison from New England Biolabs. What is DNA assembly? In the context of...the NEBuilder® HiFi DNA Assembly method from New England Biolabs® has minimal upfront requirements and allows...

  11. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    ...Many thanks to our guest blogger, David T. Riglar. David T Riglar is a postdoc at Harvard Medical School....post was contributed by guest blogger, David T. Riglar Advocating for Science Symposium and Workshop 2016...science and public policy. Follow David on Twitter @driglar.   References: 1. Alberts, Bruce, et al. "Rescuing...

  12. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    ... Kucera, M.Sc. and Eric Cantor, Ph.D. from New England Biolabs. Golden gate assembly limitations Embraced... Kucera, M.Sc. and Eric Cantor, Ph.D. from New England Biolabs. Becky Kucera is a Principal Development...Development focusing on DNA cloning and assembly, New England Biolabs, Inc.         Eric Cantor is a molecular...overseeing a team bringing new products to market, New England Biolabs, Inc.       References 1. Vladimir, Potapov...

  13. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...: The Novel of England by Edward Rutherfurd "A 10,000 year history of southern England. While the length...fiction. Two dueling magicians in the 1800s in England who help bring back actual magic (which had been...

  14. RNA Extraction Without A Kit

    Type
    Blog Post
    ...inactivate the DEPC.  Ensure that plasticware or glassware used is RNase free. RNase-free plasticware is ...readily available from scientific suppliers and glassware should be treated with a DEPC solution for 1 hour...autoclaved to remove residual DEPC. Alternatively, glassware can be baked at 180°C for at least 4 hours.   ...

  15. Tips for arabidopsis transformation

    Type
    Blog Post
    ...growing Arabidopsis are also available (Weigel and Glazebrook, 2002). Find plant plasmid resources at Addgene...recipes, and Agrobacterium strains see Weigel and Glazebrook, 2002. Tips: Do a colony PCR on your agrobacterium....70216. PubMed PMID: 17690442. 5. Weigel, D. and Glazebrook, J. (2002) Arabidopsis : a laboratory manual....

  16. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ... Greg Lohman, a biochemistry researcher at New England Biolabs. When do you need a high fidelity ligase—and...Optimization through high-throughput profiling New England Biolabs® (NEB) researchers recently published a...Greg Lohman is a biochemistry researcher at New England Biolabs, specializing in the enzymology of DNA ...

  17. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    ... that when it reaches the interface between the glass coverslip and a sample in aqueous buffer it is completely...due to the refractive index mismatch between the glass and the water-like buffer the sample is immersed...fluorophores within a few hundred nanometers of the glass/water interface. Super resolution microscopy Super...

  18. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...in their labs. Dr. Richard Roberts, CSO of New England Biolabs, gave examples of how you don’t have to...publishing in all but open access journals.  Dr. David Glass, Global Head Muscle Diseases at Novartis, opened...

  20. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...you a little bit about what I learned from a New England Biolabs (NEB) hosted workshop at Lab Central and...drawstring backpack and a double wall insulated glass to take home with us. Tips to to get the most out...
Showing: 1 - 20 of 107 results