We narrowed to 10 results for: Gla

1. Lab Safety for Biosafety Levels One and Two

   Type

   Protocol

   ...your skin, including, but not limited to broken glass or needles. Sharps containers must be thick walled...Working in a BSL-2 laboratory requires laboratory glasses in addition to coat and gloves. This lab coat should...

2. Gibson Assembly Protocol

   Type

   Protocol

   ...fragments at a time. Resources and References New England Biolabs (NEB) (Link opens in a new window) sells... window) . Gibson Assembly® is licensed to New England Biolabs by TelesisBio. Gibson DG, Young L, Chuang...

3. Protocol - pLKO.1 – TRC Cloning Vector

   Type

   Protocol

   ...Materials Material Vendor and catalog # AgeI New England Biolabs (NEB) #R0552S EcoRI NEB #R0101S NEB buffer... at all times. Use plastic pipettes in place of glass pipettes or needles. Liquid waste should be decontaminated...

4. Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs

   Type

   Protocol

   ... be used as needed. For BSL-2 work always wear glasses/goggles in addition to the BSL-1 requirements. ...

5. Protocol - How to Purify DNA from an Agarose Gel

   Type

   Protocol

   ...UV box, it is a good idea to place the gel on a glass plate if available. Unlike the plastic tray, this...

6. Protocol - How to Perform a Diagnostic Digest

   Type

   Protocol

   ...commercially available restriction enzymes, see New England Biolabs' website . Last Upload: May 18, 2018 Protocol...

7. Protocol - How to Inoculate a Bacterial Culture

   Type

   Protocol

   ...mL of LB, weigh out the following into a 500 mL glass bottle: 4 g NaCl 4 g Tryptone 2 g Yeast Extract ...

8. Protocol - How to Run an Agarose Gel

   Type

   Protocol

   ...concentration of 10 mg/mL) Gel loading buffer, New England Biolabs B7022S Procedure Pouring a Standard 1% ...

9. Handling Plasmids from Addgene - Purifying Plasmid DNA

   Type

   Protocol

   ...Potassium Acetate) 120 mL 5M Potassium acetate 23 mL glacial acetic acid 57 mL of dH 2 O Store Solution III ...

10. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type

    Protocol

    ...enzyme buffer (see (Link opens in a new window) New England Biolabs for more information about restriction ...