We narrowed to 87 results for: Gla

...columns, it’s bound to glass syringe filters. Normally DNA does not bind silica or glass, but the addition ...coaxes it into sticking to the hydrophobic glass filter. Each glass filter can capture up to 150 ug of DNA...rather a glass powder slurry that has a milk-like appearance. To make DNA stick to the glass particles...and 2) it helps DNA stick to the glass. After binding DNA, the glass particles are pelleted by centrifugation...Plasmid purification and DNA gel extraction with glass syringe filters Starting material: bacterial cultures...binding capacity. Figure 1: Purify DNA using glass filters. Image: Kim and Morrison, 2009. This ...Morrison method, you can just adjust the number of glass filters you use depending on the size of bacterial...

... Gladys Kaba from the University of Health and Allied Sciences in Ghana will be receiving the third Addgene...Duedu who were awarded similar grants back in June, Gladys will be using plasmids in creative ways that we...applications. Cervical cancer screening in Ghana Gladys is currently working to enhance cervical cancer...of high risk (HR) Human Papillomavirus (HPV). As Gladys wrote in her application, “Cervical Cancer is the...plasmids as positive controls To start her work, Gladys will improve cervical cancer screening for a limited...alongside the cytological tests currently in use. Gladys will use plasmids containing a variety of HPV genes...management. We are incredibly happy to hear that Gladys will be using plasmids in a way that can potentially...

...lab One stingy science project was to make my own glass milk for purifying cut plasmid DNA from agar slices...lifetime supply for about $10 US dollars worth of glass powder pruchased from a pottery supply house.  These... when noting that one step involves boiling the glass powder in 50% nitric acid, but I never let that ...that stop me. I only had to replenish my glass milk supplies every 10 years or so. Actually, I was happy ... smaller gel apparatus until I settled on using glass slides. With just the right amount of agarose, I... I could pour a very thin gel on a large glass slide using only surface tension.  It's a bit tricky to...

...Research Symposium organizer, David T. Riglar PhD. Here, Dr. Riglar discusses one of the six panel discussions...Many thanks to our guest blogger David T. Riglar! David T Riglar is a postdoc at Harvard Medical School....science and public policy. Follow David on Twitter @driglar.     References 1. Alberts, Bruce, et al. "Rescuing...

...in the past year. Cambridge Biological Society, England Since 1996, Cambridge Biosoc’s goal has been to... up to professors. Oxford Biological Society, England The Biological Society based at the University ...networking events. Imperial Synthetic Biology Society, England The Synthetic Biology Society is a new society ...Inspiring future scientists At Addgene, we’re incredibly glad to see these biological societies engaging with ...

...by New England Biolabs, Inc. Many thanks to our guest blogger Lydia Morrison from New England Biolabs...contributed by guest blogger Lydia Morrison from New England Biolabs. What is DNA assembly? In the context of...the NEBuilder® HiFi DNA Assembly method from New England Biolabs® has minimal upfront requirements and allows...

...Many thanks to our guest blogger, David T. Riglar. David T Riglar is a postdoc at Harvard Medical School....post was contributed by guest blogger, David T. Riglar Advocating for Science Symposium and Workshop 2016...science and public policy. Follow David on Twitter @driglar.   References: 1. Alberts, Bruce, et al. "Rescuing...

... Kucera, M.Sc. and Eric Cantor, Ph.D. from New England Biolabs. Golden gate assembly limitations Embraced... Kucera, M.Sc. and Eric Cantor, Ph.D. from New England Biolabs. Becky Kucera is a Principal Development...Development focusing on DNA cloning and assembly, New England Biolabs, Inc.         Eric Cantor is a molecular...overseeing a team bringing new products to market, New England Biolabs, Inc.       References 1. Vladimir, Potapov...

...: The Novel of England by Edward Rutherfurd "A 10,000 year history of southern England. While the length...fiction. Two dueling magicians in the 1800s in England who help bring back actual magic (which had been...

...inactivate the DEPC.  Ensure that plasticware or glassware used is RNase free. RNase-free plasticware is ...readily available from scientific suppliers and glassware should be treated with a DEPC solution for 1 hour...autoclaved to remove residual DEPC. Alternatively, glassware can be baked at 180°C for at least 4 hours.   ...

...growing Arabidopsis are also available (Weigel and Glazebrook, 2002). Find plant plasmid resources at Addgene...recipes, and Agrobacterium strains see Weigel and Glazebrook, 2002. Tips: Do a colony PCR on your agrobacterium....70216. PubMed PMID: 17690442. 5. Weigel, D. and Glazebrook, J. (2002) Arabidopsis : a laboratory manual....

... Greg Lohman, a biochemistry researcher at New England Biolabs. When do you need a high fidelity ligase—and...Optimization through high-throughput profiling New England Biolabs® (NEB) researchers recently published a...Greg Lohman is a biochemistry researcher at New England Biolabs, specializing in the enzymology of DNA ...

... that when it reaches the interface between the glass coverslip and a sample in aqueous buffer it is completely...due to the refractive index mismatch between the glass and the water-like buffer the sample is immersed...fluorophores within a few hundred nanometers of the glass/water interface. Super resolution microscopy Super...

...in their labs. Dr. Richard Roberts, CSO of New England Biolabs, gave examples of how you don’t have to...publishing in all but open access journals.  Dr. David Glass, Global Head Muscle Diseases at Novartis, opened...

...done my fair share of venting, I’m glad I did a PhD and even more glad that I thought about careers outside...

...you a little bit about what I learned from a New England Biolabs (NEB) hosted workshop at Lab Central and...drawstring backpack and a double wall insulated glass to take home with us. Tips to to get the most out...

...starting cell culture work. 3. NEB Tools by New England BioLabs In my lab we do a lot of genetic construction...experiments. Not all of our lab enzymes are made by New England BioLabs, but the NEB Tools app comes in handy when...

...supported this project with funding. Alnylam and New England Biolabs sponsored the capital construction of our... solicited seed money for the new project. New England Biolabs and Alnylam see the value to the scientific...

...mammary gland biology and neoplasia 10.3 (2005): 249-260. PubMed PMID: 16807804. Hanahan, Douglas, and Lisa...

...high-complexity reactions? The researchers over at New England Biolabs® thought otherwise! They suspected a data-driven... Golden Gate Assemblies. Image courtesy of New England Biolabs.   DAD Tools The three DAD tools used...