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Plasmid#187138Purposemammalian cell expression of 3xFLAG-HUWE1 H669QDepositorInsertHUWE1 (HUWE1 Human)
UseTags3xFLAGExpressionMammalianMutationH669QPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8-HUWE1-HECTonly
Plasmid#187119Purposeinsect cell expression of HUWE1 HECT domainDepositorInsertHUWE1 (HUWE1 Human)
UseTagsStrepExpressionInsectMutationdelta1-3992PromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_UBA/UIM-mut
Plasmid#187151PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationM1328A/F1330A/A1376G/S1382APromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_UBM-mut
Plasmid#187149PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationL2976A/P2977APromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_UBA-mut
Plasmid#187145PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationM1328A/F1330APromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_BH3-4E
Plasmid#187143PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationV1976E/V1780E/L1983E/M1987EPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_delta169-189
Plasmid#187134PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationdelta169-189PromoterAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_delta3896-3951
Plasmid#187132PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationdelta3896-3951PromoterAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_I3969A/F3982A
Plasmid#187128PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationI3969A/F3982APromoterAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_YH/GG_delta3291-3298
Plasmid#187126PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationY355G/H356G, delta3291-3298PromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_delta3291-3298
Plasmid#187124PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationdelta3291-3298PromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_YH/GG
Plasmid#187122PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationY355G/H356GPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOME0233M
Plasmid#178415PurposeReverse compl seq of pOME0134 flanked by Kpn and EcoRI for insertion into pcDNA3.1-DepositorInsertReverse complement of pOME0134 upstream MIR-SYN
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOME0232M
Plasmid#178414PurposeREVERSE complement seqeunce KSHV Downstream Mir-SYN RNA IN PCDNA3.1-DepositorInsertKSHV Reverse complement of downstream MIR-SYN
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgGabrg2
Plasmid#124857PurposeMutagenesis of Gabrg2DepositorInsertGabrg2 (Gabrg2 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTET2-RlucCP-3MS2
Plasmid#198354PurposePart of Renilla reporter system, encodes Renilla luciferase with MS2 stem loops in the 3'UTRDepositorInsertRenilla Luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterpTET2Available sinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgGrin2b
Plasmid#124851PurposeMutagenesis of Grin2bDepositorInsertGrin2b (Grin2b Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTET2-lucCP-3MS2
Plasmid#198351PurposePart of Firefly reporter system, encodes Firefly luciferase with MS2 stem loops in the 3'UTRDepositorInsertFirefly Luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterpTET2Available sinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1_UBA/UIM-mut
Plasmid#187152Purposemammalian cell expression of FLAG-HUWE1 M1328A/F1330A/A1376G/S1382ADepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationM1328A/F1330A/A1376G/S1382APromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-SaCas9-U6-sgSlc17a6
Plasmid#124860PurposeMutagenesis of Slc17a6DepositorInsertSlc17a6 (Slc17a6 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only