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Human Lentiviral sgRNA Library - Unknown Function

Pooled Library

#51043

Purpose

Sub-pool of Sabatini/Lander sgRNA library enriched for gene targets of unknown function.

Depositor

Eric Lander, David Sabatini

Article

Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only
HUNK_pCSdest

Plasmid

#53809

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

HUNK (HUNK Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
CL20_mEGFP_UNK_B4GALNT2

Plasmid

#205951

Purpose

Express mEGFP-tagged fusion protein, UNK_B4GALNT2 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

UNK_B4GALNT2 (UNK, B4GALNT2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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UNK_pGEX_noSBP

Plasmid

#228803

Purpose

Recombinant expression of UNK with mono-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Unkempt (UNK Human)

Tags

GST and HIS

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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UNK_Swap_pGEX

Plasmid

#228804

Purpose

Recombinant expression of UNK Swap with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Unkempt Zinc Fingers 4-6, 1-3 (UNK Human)

Tags

GST and SBP

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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UNK_Dual_pGEX

Plasmid

#228805

Purpose

Recombinant expression of UNK Dual with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Unkempt Zinc Fingers 4-6 x2 (UNK Human)

Tags

GST and SBP

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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UNK_6xZNF_pGEX

Plasmid

#215703

Purpose

Recombinant expression of UNK with dual-affinity tag

Depositor

Christopher Burge, Daniel Dominguez

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

UNK (UNK Human)

Tags

GST and SBP

Expression

Bacterial

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_UNK_G1

Plasmid

#127118

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA UNK (UNK Human)

Use

CRISPR

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pSkunk3-BLA

Plasmid

#61531

Purpose

Phagemid expressing streptomycin/spectinomycin resistance gene

Depositor

Marc Ostermeier

Article

Firnberg et al PLoS One. 2012;7(12):e52031. doi: 10.137

Insert

aadA

Expression

Bacterial

Promoter

lacI

Available Since

Dec. 12, 2014

Availability

Academic Institutions and Nonprofits only

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UNK_ZnF1-3_pGEX

Plasmid

#219664

Purpose

Recombinant expression of UNK ZnF1-3 with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al Nat Commun. 2024 Sep 27;15(1):8400. doi:

Insert

Unkempt Zinc Fingers 1-3

Tags

GST and SBP

Expression

Bacterial

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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UNK_ZnF4-6_pGEX

Plasmid

#219665

Purpose

Recombinant expression of UNK ZnF4-6 with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al Nat Commun. 2024 Sep 27;15(1):8400. doi:

Insert

Unkempt Zinc Fingers 4-6

Tags

GST and SBP

Expression

Bacterial

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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HUNK gRNA (BRDN0001148239)

Plasmid

#75940

Purpose

3rd generation lentiviral gRNA plasmid targeting human HUNK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HUNK (HUNK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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HUNK gRNA (BRDN0001146355)

Plasmid

#75939

Purpose

3rd generation lentiviral gRNA plasmid targeting human HUNK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HUNK (HUNK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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HUNK gRNA (BRDN0001145500)

Plasmid

#75941

Purpose

3rd generation lentiviral gRNA plasmid targeting human HUNK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HUNK (HUNK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
HUNK_pCSdest

Plasmid

#53809

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

HUNK (HUNK Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
px458_2A_GFP_sgRNA_UNK_G1

Plasmid

#127118

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA UNK (UNK Human)

Use

CRISPR

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pSkunk3-BLA

Plasmid

#61531

Purpose

Phagemid expressing streptomycin/spectinomycin resistance gene

Depositor

Marc Ostermeier

Article

Firnberg et al PLoS One. 2012;7(12):e52031. doi: 10.137

Insert

aadA

Expression

Bacterial

Promoter

lacI

Available Since

Dec. 12, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
HUNK gRNA (BRDN0001148239)

Plasmid

#75940

Purpose

3rd generation lentiviral gRNA plasmid targeting human HUNK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HUNK (HUNK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
HUNK gRNA (BRDN0001146355)

Plasmid

#75939

Purpose

3rd generation lentiviral gRNA plasmid targeting human HUNK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HUNK (HUNK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
HUNK gRNA (BRDN0001145500)

Plasmid

#75941

Purpose

3rd generation lentiviral gRNA plasmid targeting human HUNK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HUNK (HUNK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge