We narrowed to 15,052 results for: pET

1. pET28a Gx-NL3

   Plasmid

   #169108

   Purpose

   Expresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

   Depositor

   Maarten Merkx

   Article

   Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

   Insert

   protein G - NanoLuc - NanoLuc - NanoLuc
   

   Tags

   his-tag and strep-tag

   Expression

   Bacterial

   Mutation

   Ala 24 stop

   Promoter

   T7

   Available Since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28b-PU.1-ETS

   Plasmid

   #85734

   Purpose

   Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272

   Depositor

   Gregory Poon

   Article

   Wang et al J Biol Chem. 2014 Aug 1;289(31):21605-16

   Insert

   ETS domain of murine PU.1 (residues 167-272) (Spi1 Human, Mouse)
   

   Tags

   6xHis following thrombin cleavage site LVPR|G

   Expression

   Bacterial

   Available Since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-HA-CPDSalI

   Plasmid

   #38254

   Depositor

   Matthew Bogyo, Aimee Shen

   Article

   Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

   Type

   Empty backbone

   Tags

   6xHis, HA, and Vibrio cholerae MARTX toxin cystei…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-HsSENP6

   Plasmid

   #71469

   Purpose

   Encodes human full length SENP6 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

   Depositor

   Jorge Eduardo Azevedo

   Article

   Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

   Insert

   SUMO1/sentrin specific peptidase 6 (SENP6 Human)
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Gene optimised for E. coli expression (Genscript)

   Promoter

   T7

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pET28-mHP1alpha

   Plasmid

   #181911

   Purpose

   Bacterial protein expression vector for HP1alpha

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   mHP1alpha (Cbx5 Mouse)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET29b-NpR5599

   Plasmid

   #45740

   Depositor

   Emily Balskus, Christopher T Walsh

   Article

   Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

   Insert

   NpR5599 (Npun_R5599 Nostoc punctiforme)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 3, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pDpsTE-Ypet

   Plasmid

   #98277

   Purpose

   mammalian expression of DpsTE-Ypet

   Depositor

   Mike Heilemann

   Article

   Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

   Insert

   Dps
   

   Tags

   Ypet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21a Hbb

   Plasmid

   #26639

   Depositor

   Phoebe Rice

   Article

   Mouw et al Mol Microbiol. 2007 Mar . 63(5):1319-30.

   Insert

   Hbb (hbb Borrelia burgdorferi)
   

   Expression

   Bacterial

   Available Since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

9. Ypet-RBD

   Plasmid

   #105291

   Purpose

   To measure Rho activity by binding and FRET analysis

   Depositor

   Kenneth Yamada

   Article

   Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

   Insert

   RBD (Rtkn Mouse)
   

   Tags

   Ypet

   Expression

   Mammalian

   Mutation

   aa encoding 7-89

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-UVRAG CC

    Plasmid

    #24400

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    UVRAG (UVRAG Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains on the CC domain, amino acids 200-270

    Available Since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pET29a-hNABP1-C

    Plasmid

    #128311

    Purpose

    Express hNABP1 C-terminus (125–204 aa) in E. coli with a C-terminal His-tag

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

    Insert

    Human nucleic acid-binding protein 1 (hNABP1)-C terminus (125-204 aa) (NABP1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21a - Mtb-TF

    Plasmid

    #111513

    Purpose

    Expresses M. tuberculosis Trigger Factor with a C-terminal His tag

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

    Insert

    Trigger Factor
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET51b(+)_S-Luc_CLIP

    Plasmid

    #113923

    Purpose

    Plasmid encoding the bacterial expression of the chimeric SNAP-NanoLuc fusion dubbed S-Luc c-terminally linked to CLIP-tag for BRET

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    S-Luc_CLIP
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET22b-HA-CPDSalI

    Plasmid

    #38253

    Depositor

    Matthew Bogyo, Aimee Shen

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Type

    Empty backbone

    Tags

    6xHis, HA, and Vibrio cholerae MARTX toxin cystei…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21-tVYN-Nt∆

    Plasmid

    #154431

    Purpose

    Plasmid used to express the tVYN-Nt∆ luminescent biosensor for use in live cell cyclic di-GMP detection

    Depositor

    Ming Hammond

    Article

    Dippel et al ACS Chem Biol. 2020 Mar 25. doi: 10.1021

    Insert

    VenusΔC12-NtYcgRΔ-NLucΔ4
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21-MRPP1_native+

    Plasmid

    #67865

    Purpose

    bacterial expression of TRMT10C, full coding sequence (mitoch. form)

    Depositor

    Walter Rossmanith

    Article

    Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

    Insert

    MRPP1 (TRMT10C Human)
    

    Expression

    Bacterial

    Mutation

    mitoch. form (contains aa 40-403)

    Promoter

    T7

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET32a-hAREG

    Plasmid

    #199229

    Purpose

    Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

    Depositor

    Nilson Zanchin

    Article

    Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

    Insert

    Amphiregulin preproprotein (AREG Human)
    

    Tags

    TrxA-6xHis-S-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Ubp8/PET32a

    Plasmid

    #26965

    Depositor

    Cynthia Wolberger

    Article

    Samara et al Science. 2010 May 21. 328(5981):1025-9.

    Insert

    Ubp8 (UBP8 Budding Yeast)
    

    Tags

    His6 and Trx

    Expression

    Bacterial

    Mutation

    TEV site is added t the 5' end and 4 alanine…

    Available Since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pET28a-HsSENP5

    Plasmid

    #71468

    Purpose

    Encodes human full length SENP5 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 5 (SENP5 Human)
    

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Gene optimised for E. coli expression (Genscript)

    Promoter

    T7

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

20. PL-452 C-YPet

    Plasmid

    #19177

    Depositor

    Patrik Verstreken

    Article

    Venken et al Nucleic Acids Res. 2008 Oct;36(18):e114.

    Insert

    YPet
    

    Use

    Cre/Lox

    Available Since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only