We narrowed to 63,866 results for: cell

1. pFX-H2B-Venus

   Plasmid

   #175840

   Purpose

   Express Venus with nucleus-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   Venus with nucleus-targeting sequence
   

   Tags

   H2B (human histone 2B)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. 3xFLAG-dCas9/MSCV-EGFP

   Plasmid

   #82613

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al Genes Cells. 2018 Feb 26. doi: 10.1111/g

   Insert

   3xFLAG-dCas9
   

   Use

   Retroviral

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. EK0307 CMV-TO-BFPmut-stop-3UTR(Bdnf) (FLP-IN)

   Plasmid

   #191145

   Purpose

   Expression of a synthetic construct with 3' UTR of murine Bdnf in the 3' UTR of an inactive BFP in mammalian cells.

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   3UTR(Bdnf)
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV-TO

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFX-H2B-iRFP

   Plasmid

   #175842

   Purpose

   Express iRFP with nucleus-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   iRFP with nucleus-targeting sequence
   

   Tags

   H2B (human histone 2B)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG CAG Glucagon-ICAM1-3xHA-pre-mGRASP

   Plasmid

   #153008

   Purpose

   Expresses glucagon peptide on mammalian cell surface

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   Glucagon-ICAM1-pre-mGRASP
   

   Tags

   3x HA

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST-CMV 3xFLAG-LC3C-GFP

   Plasmid

   #123110

   Purpose

   Expresses 3xFLAG-LC3C-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3C

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
   

   Tags

   3xFLAG and EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK5RS-HERCD533

   Plasmid

   #65224

   Purpose

   Expression of dominant negative EGFR in mammalian cells

   Depositor

   Axel Ullrich

   Article

   Redemann et al Mol Cell Biol. 1992 Feb;12(2):491-8.

   Insert

   HERCD533 (EGFR Human)
   

   Expression

   Mammalian

   Mutation

   deleted AA 654-1186

   Promoter

   CMV

   Available Since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

8. EFF-1::V5

   Plasmid

   #132961

   Purpose

   Expresses EFF-1 A full length protein in mammalian cells

   Depositor

   Benjamin Podbilewicz

   Article

   Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581.

   Insert

   EFF-1::V5
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF-pIgH-ESCargo*

   Plasmid

   #140159

   Purpose

   Expresses a regulatable secretory cargo (without an ER export signal) for mammalian cells

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   piGH-DsRed-Express2-FKBP(LV)(C22V)
   

   Tags

   ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. NSK024 SFFV-mCherry-SG(p53R248Q.s2)SG-EGFP (FLP-IN)

    Plasmid

    #191169

    Purpose

    Expression of a sensor for p53 mutant R248Q in mammalian cells; mCherry marker, EGFP output

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:p53(R248Q).s2:EGFP
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLJC2-UMPS-3xFLAG

    Plasmid

    #87975

    Purpose

    3rd generation lentiviral vector for Expression of UMPS in mammalian cells

    Depositor

    David Sabatini

    Article

    Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

    Insert

    UMPS (UMPS Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUBC_V5_MS2-mVenus

    Plasmid

    #107606

    Purpose

    Transient expression of humanized MS2 (V75E/A81G), fused to mVenus in mammalian cells, under a hUBC promoter, V5 tag

    Depositor

    John Rinn

    Article

    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

    Insert

    MS2-mVenus
    

    Use

    Synthetic Biology

    Tags

    V5 and mVenus

    Mutation

    MS2: V75E/A81G

    Promoter

    human UBC

    Available Since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFX-GalT-EGFP

    Plasmid

    #174471

    Purpose

    Express EGFP with golgi apparatus-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    EGFP with golgi apparatus-targeting sequence
    

    Tags

    β1,4-galactose transferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLib6.4-Aalb_743

    Plasmid

    #176662

    Purpose

    Expression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Tet-O-FUW-Nkx6.1

    Plasmid

    #45846

    Depositor

    Marius Wernig

    Article

    Yang et al Nat Biotechnol. 2013 May;31(5):434-9. do

    Insert

    Nkx6.1 (Nkx6-1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    tet-ON

    Available Since

    July 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJBL7085

    Plasmid

    #167249

    Purpose

    orthogonal mRFP reporter output for cell-free expression

    Depositor

    Julius Lucks

    Article

    Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

    Insert

    pAKSIRV-mRFP1
    

    Use

    Synthetic Biology

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2-PLK1-mCherry

    Plasmid

    #127154

    Purpose

    Expresses mCherry PLK1 in mammalian cells and zebrafish embryos

    Depositor

    Heidi Hehnly

    Article

    Colicino et al Mol Biol Cell. 2019 Jun 15;30(13):1598-1

    Insert

    PLK1-mCherry (PLK1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-mGold-Rab4a-C-7

    Plasmid

    #158002

    Purpose

    Visualization of endosome in mammalian cells.

    Depositor

    Francois St-Pierre

    Article

    Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

    Insert

    Rab4a (RAB4A Human)
    

    Tags

    mGold

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF Itk myc

    Plasmid

    #20705

    Depositor

    Leslie Berg

    Article

    Bunnell et al J Biol Chem. 2000 Jan 21. 275(3):2219-30

    Insert

    Itk (Itk Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    March 17, 2009

    Availability

    Academic Institutions and Nonprofits only

20. Tet-O-FUW-Zfp536

    Plasmid

    #45845

    Depositor

    Marius Wernig

    Article

    Yang et al Nat Biotechnol. 2013 May;31(5):434-9. do

    Insert

    Zfp536 (Zfp536 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Tet-ON

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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