We narrowed to 12,188 results for: LEA

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pAG415Gal_Gcn4 Aro2+

Plasmid

#232982

Purpose

Galactose iduced expression of Gcn4 Aro2+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro2+

Tags

TEV cleavage site

Expression

Yeast

Mutation

N116F, S117E, E119N, P129Q, T132Y

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Aro3+

Plasmid

#232983

Purpose

Galactose iduced expression of Gcn4 Aro3+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro3+

Tags

TEV cleavage site

Expression

Yeast

Mutation

D103Y, N112T, L113Y, S117F, K140R

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WW+GFP

Plasmid

#233003

Purpose

Galactose iduced expression of Gcn4 WW+GFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WW+

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

E109N, S117D, T121W, N126W, T132W

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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ppAG415Gal_Gcn4 L113A+

Plasmid

#232972

Purpose

Galactose iduced expression of Gcn4 L113A in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 L113A

Tags

TEV cleavage site

Expression

Yeast

Mutation

L113A, E114Y, N126H, D127E, S136A

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 StoL

Plasmid

#232981

Purpose

Galactose iduced expression of Gcn4 StoL in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 StoL

Tags

TEV cleavage site

Expression

Yeast

Mutation

S104L, S117L, S136L, S144L

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 R3W

Plasmid

#232975

Purpose

Galactose iduced expression of Gcn4 R++ W+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 R++ W+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

D103R, N126W, D133R, K143R

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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GB4003

Plasmid

#215248

Purpose

Module for the constitutive expression of PIF6 and PhyB:VP16

Depositor

Diego Orzaez

Publication

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

PIF6-PhyB:VP16

Use

Synthetic Biology

Mutation

V234L in PIF6- please see dep. sequence, BsaI and…

Available since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pAIP4_5GUQ

Plasmid

#234171

Purpose

Heterologous protein expression of ASCH domain-containing ribonuclease in Escherichia coli

Depositor

Christopher Bahl

Publication

Cárcamo Noriega et al Protein Sci. 2026 Sep;35(9):e70746. doi:

Insert

5GUQ

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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esgRNA_NbATML1-2pro and NbATML1-1pro

Plasmid

#231155

Purpose

T-DNA encoding TRV2 with mobile gRNAs targeting NbATML1-2pro and NbATML1-1pro

Depositor

Daniel Voytas

Publication

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

mobile gRNAs targeting NbATML1-2pro and NbATML1-1pro

Expression

Plant

Promoter

PEBV sub-genomic

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-Puro-P2A-NES/PKI-mNeonGreen2x-NLS/FUS

Plasmid

#228400

Purpose

Encodes NES/PKI-mNeonGreen2x-NLS/FUS which is a tandem of mNeongreen with an N-terminal PKI NES and a C-terminal FUS NLS that shuttles between the nucleus and the cytoplasm

Depositor

Dirk Daelemans

Publication

Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

Insert

NES/PKI-mNeonGreen2x-NLS/FUS

Expression

Mammalian

Promoter

CMV

Available since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4-CMV-NLS/cMyc-AcGFP2x-NES/iKB2-P2A-Puro

Plasmid

#228398

Purpose

Encodes NLS/cMyc-AcGFP2x-NES/iKB2 which is a tandem of AcGFP with an N-terminal cMyc NLS and a C-terminal iKB2 NES that shuttles between the nucleus and the cytoplasm

Depositor

Dirk Daelemans

Publication

Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

Insert

NLS/cMyc-AcGFP2x-NES/iKB2

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-F-box:gs10:FRB:HA

Plasmid

#228531

Purpose

F-box domain of human Skp2 protein in fusion with FRB domain through a flexible glycine-serine linker.

Depositor

Roman Jerala

Publication

Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

Insert

F-box:gs10:FRB:HA (SKP2 Human, Synthetic)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc:FRB:gs10:Cul1(dC501)

Plasmid

#228533

Purpose

A fragment of humanCul1 protein in fusion with FRB domain through a flexible glycine-serine linker.

Depositor

Roman Jerala

Publication

Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

Insert

myc:FRB:gs10:Cul1(dC501) (CUL1 Human, Synthetic)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCR8/GW_TOPO_Cdk13_Untagged

Plasmid

#127342

Purpose

Untagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into Gateway entry vector

Depositor

Phil Sharp

Publication

Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

Insert

Cyclin Dependent Kinase 13 (Cdk13 Mouse)

Use

Entry vector with transgene for gateway cloning

Mutation

Base pairs 1-1554 of transgene are codon optimize…

Promoter

None

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-nucGFP11-3xPax7gRNA

Plasmid

#224570

Purpose

Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

Depositor

Erica Hutchins

Publication

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

GFP11-H2B

Use

CRISPR

Tags

Histone H2B (nuclear localization)

Mutation

Codon optimized

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pACE SOX (ORF37) K246A

Plasmid

#192051

Purpose

Expresses a catalytic mutant of SOX (K246A)

Depositor

Britt Glaunsinger

Publication

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (K246A)

Use

Unspecified

Mutation

Catalytic mutant K246A

Promoter

CMV

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pACE SOX (ORF37) K250A

Plasmid

#192052

Purpose

Expresses a DNA binding mutant K250A (ORF37)

Depositor

Britt Glaunsinger

Publication

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (K250A)

Use

Unspecified

Mutation

DNA binding mutant K250A

Promoter

CMV

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Mito(OM)-deltaCKAR

Plasmid

#222431

Purpose

FRET-based reporter for monitoring deltaPKC activity at the mitochondrial outer membrane in cells.

Depositor

Alexandra Newton

Publication

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Mitochondrial outer membrane delta-selective C kinase activity reporter

Tags

CFP and YFP

Expression

Mammalian

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a-DIO-PSAML-141F,Y115F- GlyR-2A-FusionRed

Plasmid

#226590

Purpose

Pharmacologically Selective Actuator Module, chimeric ligand-gated ion channels that can be used to manipulate electrophysiological activity.

Depositor

D. James Surmeier

Insert

PSAML-141F,Y115F-GlyR

Use

AAV

Tags

P2A-Fusion Red

Expression

Mammalian

Promoter

EF1a

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBacI-KDAC7 CD

Plasmid

#224345

Purpose

Expresses human KDAC7 (HDAC7) catalytic domain in insect cells

Depositor

Terry Watt

Publication

Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

Insert

KDAC7 (HDAC7 Human)

Tags

TEV-cleavable His6

Expression

Insect

Mutation

Only includes residues 521-942 (catalytic domain).

Promoter

Polyhedrin

Available since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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