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pVC0_2_04_PJ23102

Plasmid

#249865

Purpose

constitutive promoter, lv0, 5th strongest

Depositor

Georg Fritz

Publication

Faber et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

constitutive promoter, lv0, 5th strongest

Use

Synthetic Biology

Available since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pH35C-gRNA-Cas9

Plasmid

#246794

Purpose

Express Cas9 protein and gRNA in plant cells (protoplast or callus) for Cas9-mediated editing

Depositor

Caixia Gao

Publication

Li et al Nat Biotechnol. 2026 Jun 5. doi: 10.1038

Insert

gRNA, Cas9, HygR

Use

CRISPR

Available since

March 17, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx2_WT_GFP (ABE8e_L34W_nCas9_WT)

Plasmid

#251549

Purpose

Mammalian expression plasmid encoding an nCas9-WT adenine base editor (ABE8e) carrying the L34W mutation in the deaminase domain

Depositor

George Church

Publication

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_L34W-nCas9_WT

Use

CRISPR

Expression

Mammalian

Mutation

ABEx2 L34W

Available since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx2_SpRY (ABE8e_L34W_nCas9_SpRY)

Plasmid

#251545

Purpose

Mammalian expression plasmid encoding an nCas9-SpRY adenine base editor (ABE8e) carrying the L34W mutation in the deaminase domain

Depositor

George Church

Publication

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_L34W-nCas9_SpRY

Use

CRISPR

Expression

Mammalian

Mutation

ABEx2 L34W

Available since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx4_WT_GFP (ABE8e_V28C_M151E_nCas9_WT)

Plasmid

#251551

Purpose

Mammalian expression plasmid encoding an nCas9-WT adenine base editor (ABE8e) carrying the V28C and M151E mutations in the deaminase domain

Depositor

George Church

Publication

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_V28C_M151E-nCas9_WT

Use

CRISPR

Expression

Mammalian

Mutation

ABEx4 V28C_M151E

Available since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx3_WT_GFP (ABE8e_M151E_nCas9_WT)

Plasmid

#251550

Purpose

Mammalian expression plasmid encoding an nCas9-WT adenine base editor (ABE8e) carrying the M151E mutation in the deaminase domain

Depositor

George Church

Publication

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_M151E-nCas9_WT

Use

CRISPR

Expression

Mammalian

Mutation

ABEx3 M151E

Available since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pANC79b(P3-Myc-nTEV-IRES-EBFP)

Plasmid

#248357

Purpose

Lentiviral vector that can express P3 along with N terminal split TEV. Expressed off of CMV tet ON promoter with EBFP marker

Depositor

Arnab Mukherjee

Publication

Chacko et al Sci Adv. 2026 Jan 16;12(3):eaec1211. doi

Insert

P3-Myc-nTEV

Use

Lentiviral

Promoter

tight TRE

Available since

Jan. 30, 2026

Availability

Academic Institutions and Nonprofits only

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pANC79a(P4-GGGSGGG-cTEV-HA-IRES-dTomato)

Plasmid

#248356

Purpose

Lentiviral vector that can express P4 along with C terminal split TEV. Expressed off of CMV tet ON promoter with dTomato marker

Depositor

Arnab Mukherjee

Publication

Chacko et al Sci Adv. 2026 Jan 16;12(3):eaec1211. doi

Insert

P4-GGGSGGG-cTEV-HA

Use

Lentiviral

Promoter

tight TRE

Available since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pET-21(+)-His-zf438-NB23

Plasmid

#247372

Purpose

Express zf438-NB23 that bind to SARS-CoV-19 Spike protein cooperatively with NB15-T7 RNAP.

Depositor

Sebastian Maerkl

Publication

Lee et al bioRxiv 2025.06.18.660355

Insert

His-zf438-NB23

Tags

6xHis

Expression

Bacterial

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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C-term_C-myc (4a)

Plasmid

#238907

Purpose

Plasmid insert contains the C-myc C-terminal tag sequence for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Publication

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

C-term_C-myc

Use

Synthetic Biology

Available since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pJM220-PphoA-mNeongreen-J23116-mScarletI

Plasmid

#240200

Purpose

MiniTn7 donor plasmid for constructing a ratiometric reporter for P limitation in Pseudomonas synxantha. A weak Anderson promoter (J23116) drives expression of mScarlet-I

Depositor

Richard Murray

Publication

Larsson et al ACS Synth Biol. 2024 Jan 19;13(1):384-39

Inserts

mNeongreen
mScarlet-I

Promoter

J23116 and PphoA

Available since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pHK302.6

Plasmid

#235471

Purpose

Message phagemid carrying sgRNA6 (prom. J23110, backbone pBR322)

Depositor

Yolanda Schaerli

Publication

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA6

Use

Synthetic Biology

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHK002.1

Plasmid

#235460

Purpose

Message phagemid carrying sgRNA1 (prom. J23119, backbone pBR322)

Depositor

Yolanda Schaerli

Publication

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA1

Use

Synthetic Biology

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHK002.3

Plasmid

#235462

Purpose

Message phagemid carrying sgRNA3 (prom. J23119, backbone pBR322)

Depositor

Yolanda Schaerli

Publication

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA3

Use

Synthetic Biology

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHK002.4

Plasmid

#235463

Purpose

Message phagemid carrying sgRNA4 (prom. J23119, backbone pBR322)

Depositor

Yolanda Schaerli

Publication

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA4

Use

Synthetic Biology

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHK002.5

Plasmid

#235464

Purpose

Message phagemid carrying sgRNA5 (prom. J23119, backbone pBR322)

Depositor

Yolanda Schaerli

Publication

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA5

Use

Synthetic Biology

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHK002.6

Plasmid

#235465

Purpose

Message phagemid carrying sgRNA6 (prom. J23119, backbone pBR322)

Depositor

Yolanda Schaerli

Publication

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA6

Use

Synthetic Biology

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHK302.1

Plasmid

#235466

Purpose

Message phagemid carrying sgRNA1 (prom. J23110, backbone pBR322)

Depositor

Yolanda Schaerli

Publication

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA1

Use

Synthetic Biology

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHK302.2

Plasmid

#235467

Purpose

Message phagemid carrying sgRNA2 (prom. J23110, backbone pBR322)

Depositor

Yolanda Schaerli

Publication

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA2

Use

Synthetic Biology

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHK302.3

Plasmid

#235468

Purpose

Message phagemid carrying sgRNA3 (prom. J23110, backbone pBR322)

Depositor

Yolanda Schaerli

Publication

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA3

Use

Synthetic Biology

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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