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pLCHKOv5

Plasmid

#237452

Purpose

Lentiviral backbone for expressing doxycycline (Dox)-inducible, Pol II-driven hybrid guide (hg)RNAs.

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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E. coli DNA Polymerase III subunit Delta' (holB) in pet28PP

Plasmid

#217410

Purpose

Expresses E. coli DNA Polymerase III subunit delta' (holb) with a His-tag in bacterial cells

Depositor

Brian Kelch

Article

Landeck et al J Biol Chem. 2024 Apr;300(4):107166. doi

Insert

DNA polymerase III subunit Delta' (holB E. coli)

Tags

His

Expression

Bacterial

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1_-FCRL3-3xFLAG-puro

Plasmid

#248155

Purpose

Lentiviral vector expressing human FCRL3 with 3xFLAG tags

Depositor

Silvia Monticelli

Article

Bianchi et al J Exp Med (2026) 223 (1): e20242474.

Insert

FCRL3 (FCRL3 Human)

Use

Lentiviral

Tags

3x FLAG

Mutation

A 3xFLAG epitope tag is fused in frame to the C-t…

Promoter

EF1_

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pHR Myc-Oskar-FusionRed-FRB-iLID

Plasmid

#223289

Purpose

The Oskar germ granule protein tagged with optogenetic and chemogenetic tags for recruitment of desired cargo. Used to demonstrate condensate dissolution using the OptoMBP system.

Depositor

Jared Toettcher

Article

Brumbaugh-Reed et al Nat Commun. 2024 Aug 7;15(1):6717. doi:

Insert

myc-Oskar-FusionRed-FRB-iLID

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr201 RNF146 deltaWWE

Plasmid

#240331

Purpose

Entry vector for Gateway with RNF146 (WWE domain deletion)

Depositor

Junjie Chen

Article

Nie et al Mol Cell Proteomics. 2020 Dec;19(12):201

Insert

RNF146 (RNF146 Human)

Expression

Bacterial

Mutation

WWE domain deletion

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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FUGW-EGFP-mPkm2

Plasmid

#242812

Purpose

Lentiviral vector expressing mouse Pkm2 tagged with an N-terminal EGFP

Depositor

Ronald Hart

Article

Sun et al Nat Commun. 2025 Oct 21;16(1):9312. doi:

Insert

pyruvate kinase, muscle (Pkm Mouse)

Use

Lentiviral

Tags

EGFP

Promoter

Ubiquitin C

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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ER-GFP pLVX CMVpuro

Plasmid

#246912

Purpose

Lentiviral-based expression of EGFP with an ER retention signal sequence in mammalian cells

Depositor

William Kaelin

Article

Lampson et al Cell. 2024 Apr 25;187(9):2209-2223.e16.

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pNoRBP.iGlucoSnFR2.mIRFP670nano3

Plasmid

#244107

Purpose

Bacterial expression of green glucose sensor with inert mIRFP670nano3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Tags

mIRFP670nano3

Expression

Bacterial

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr201 RNF146 deltaRING

Plasmid

#240332

Purpose

Entry vector for Gateway with RNF146 (RING domain deletion)

Depositor

Junjie Chen

Article

Nie et al Mol Cell Proteomics. 2020 Dec;19(12):201

Insert

RNF146 (RNF146 Human)

Expression

Bacterial

Mutation

RING domain deletion

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pSB02

Plasmid

#230991

Purpose

B. dendrobatidis plasmid for integration of target sequence at URA3 locus, blasticidin S selection

Depositor

Lillian Fritz-Laylin

Article

Brody et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

BdURA3_LHA2-Hsbsr-ScADH1ter

Use

Bd gene targeting vector

Tags

bsr

Expression

Bacterial

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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EBFP2-Kindlin2-3KA

Plasmid

#242823

Purpose

Encodes human kindlin2-3KA mutant and N-terminal EBFP2 tag

Depositor

Cheng-han Yu

Article

Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

Insert

Kindlin2 (FERMT2 Human)

Expression

Mammalian

Mutation

K385A, K386A, K393A

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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EBFP2-K1K2

Plasmid

#242819

Purpose

Encodes human kindlin1 containing kindlin2 F3 domain and N-terminal EBFP2 tag

Depositor

Cheng-han Yu

Article

Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

Insert

Kindlin1 (Fermt1 Human)

Expression

Mammalian

Mutation

Kindlin1 chimera containing the F3 domain of kind…

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr 201_MGA

Plasmid

#240327

Purpose

Entry vector for Gateway with MGA

Depositor

Junjie Chen

Article

Nie et al Genes Dev. 2022 Sep 1;36(17-18):1016-103

Insert

MGA (MGA Human)

Expression

Bacterial

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-Gnai3

Plasmid

#245997

Purpose

Express HA N-terminal tagged mouse Galphai3 transiently via transfection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gnai3 (Gnai3 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-GFP

Plasmid

#243759

Purpose

AAV plasmid expressing GFP under the CAG promoter.

Depositor

Andrew Arrant

Article

Nader et al Sci Rep. 2025 Jul 19;15(1):26217. doi: 1

Insert

Enhanced Green Fluorescent Protein

Use

AAV

Promoter

CAG

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(ER).iGlucoSnFR2.HaloTag

Plasmid

#244076

Purpose

ER targeted expression of green glucose sensor with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.HaloTag

Use

AAV

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pNoRBP.iGlucoSnFR2.H348H.HaloTag

Plasmid

#244108

Purpose

Bacterial expression of green glucose sensor (high affinity) with inert HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.H348H.HaloTag

Tags

HaloTag

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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EBFP2-K2K1

Plasmid

#242820

Purpose

Encodes human kindlin2 containing kindlin1 F3 domain and N-terminal EBFP2 tag

Depositor

Cheng-han Yu

Article

Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

Insert

Kindlin2 (FERMT2 Human)

Expression

Mammalian

Mutation

Kindlin2 chimera containing the F3 domain of kind…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-Nsp3(1-111)

Plasmid

#242947

Purpose

GFP and the Ubl1 domain of the SARS-CoV-2 Nsp3 protein (aa 1-111)

Depositor

Benjamin Schuster

Article

Favetta et al bioRxiv [Preprint]. 2025 Jun 10:2024.10.

Insert

GFP-Nsp3(1-111) (ORF1ab SARS-CoV-2)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Integrin β6β1-EGFP

Plasmid

#242829

Purpose

Encodes human integrin β6 chimera containing integrin β1 cytoplasmic domain and C-terminal EGFP tag

Depositor

Cheng-han Yu

Article

Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

Insert

Integrin subunit beta 6, transcript variant 1 (ITGB6 Human)

Expression

Mammalian

Mutation

Integrin β6 chimera containing the cytoplasmic ta…

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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