We narrowed to 18,769 results for: Mut

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p2lox-cAMPr R739A

Plasmid

#102618

Purpose

cAMPr with a point mutation R739A which theoretically increases its affinity for cAMP

Depositor

Justin Blau

Article

Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

Insert

cAMPr

Use

Cre/Lox

Expression

Mammalian

Mutation

R739A

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pPuro3.1(+)_Strep-Lrrk2(G2019S)-Myc

Plasmid

#161585

Purpose

expression of Myc-tagged mouse Lrrk2 containing Parkinson's associated G2019S mutation

Depositor

Maik Hintze

Insert

Lrrk2 (Lrrk2 Mouse)

Tags

Myc tag and StrepII

Expression

Mammalian

Mutation

G2019S

Promoter

CMV

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pPSmOrange-C1

Plasmid

#31899

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S (mutations…

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pNR230

Plasmid

#79546

Purpose

BxbI attB-attP. GFP will express if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pNR276

Plasmid

#79577

Purpose

TP901 attB-attP. GFP will express if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pNR239

Plasmid

#79576

Purpose

A118 attB-attP. GFP will express if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pKJ0377

Plasmid

#228712

Purpose

Mammalian expression of human codon-optimized enhanced Bxb1 (epBxb1 mutant T166R)

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Science 0, eadr6006

Insert

Bxb1_T166R

Expression

Mammalian

Mutation

T166R

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUPD2-HR1bR181A R185A-C (B3-B4)

Plasmid

#211855

Purpose

CDS for mutant HR1b degron in domestication vector

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

HR1b

Use

Synthetic Biology

Mutation

R181A R185A

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CA-FLEX

Plasmid

#38042

Purpose

Cre activates gene of interest, using 4 mutated lox sites

Depositor

Naoshige Uchida

Article

Watabe-Uchida et al Neuron. 2012 Jun 7;74(5):858-73.

Type

Empty backbone

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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MSCV-FoxP3-R337Q/A372S-IRES-Thy1.1

Plasmid

#229619

Purpose

Mammalian expression of full length mouse FoxP3 (R337Q, A372S)

Depositor

Sun Hur

Insert

full length FoxP3, carrying double mutation R337Q/A372S (Foxp3 Mouse)

Expression

Mammalian

Mutation

R337Q/A372S

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLG169

Plasmid

#124367

Purpose

Xylose inducible system with the CRE site for expression in B. subtilis

Depositor

Christopher Voigt

Article

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Insert

gfpmut2 (NEWENTRY Synthetic)

Expression

Bacterial

Promoter

Pxyl

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pFLAG-CMV2-Hsp27-S15A/S78A/S82A

Plasmid

#84997

Purpose

Hsp27 expression plasmid

Depositor

Ugo Moens

Insert

Hsp27 (HSPB1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Serine 15 and 78 and 82 mutated to Alanine

Promoter

CMV

Available Since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCZGY2849

Plasmid

#74112

Purpose

Enhanced mutant version of membrane targeted PH-miniSOG (Q103L) entry clone for more efficient ablation of cells when expressed under desired promoter.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

miniSOG(Q103L)

Use

Gateway topo cloning

Tags

PH

Available Since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgKcnq3

Plasmid

#159911

Purpose

Mutagenesis of Kcnq3

Depositor

Larry Zweifel

Insert

Kcnq3 (Kcnq3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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p8.9 NdeltaSB P90A

Plasmid

#132930

Purpose

p8.9 NdeltaSB bearing P90A mutant capsid sequence

Depositor

Jeremy Luban

Article

De Iaco et al Retrovirology. 2011 Dec 6;8:98. doi: 10.

Insert

gag P90A and pol

Use

Lentiviral

Mutation

P90A

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pPuro3.1(+)_Strep-Lrrk2(D1994S)-Myc

Plasmid

#161584

Purpose

expression of Myc-tagged kinase-dead mouse Lrrk2 containing D1994S mutation

Depositor

Maik Hintze

Insert

Lrrk2 (Lrrk2 Mouse)

Tags

Myc tag and StrepII

Expression

Mammalian

Mutation

D1994S

Promoter

CMV

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBNG2

Plasmid

#149473

Purpose

Broad-Host range expression vector, pBBR origin, NahR(AM) repressor, PSalTTC promoter, GFPmut3b, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

NahR(AM)-GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PSalTTC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAraTMwt

Plasmid

#47514

Purpose

Contains A2B L980A TMCY mutant, expresses wt AraC for heterodimer competition assay

Depositor

Bryan Berger

Article

Su et al J Mol Biol. 2013 Jul 19. pii: S0022-2836

Insert

Integrin alpha2B L980A TM-CYTO

Expression

Bacterial

Promoter

Tac

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pMtBS

Plasmid

#32226

Depositor

Christopher Buck

Article

Schowalter et al PLoS Pathog. 2011 Jul;7(7):e1002161. Epu

Insert

MCV Small t antigen

Expression

Mammalian

Mutation

Splice site was silently mutated, and a restricti…

Available Since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pH2B-PSmOrange

Plasmid

#31920

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Tags

H2B

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

Available Since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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