We narrowed to 31,420 results for: Eng

1. pBiT3.1-C_ARPC1B:1-372

   Plasmid

   #211268

   Purpose

   Mammalian expression of full-length human ARPC1B. C-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   ARPC1B:M1-K372 (ARPC1B Human)
   

   Use

   Tags

   GSSGGSSGVSGWRLFKKIS

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBiT3.1-C_ELP2:1-826

   Plasmid

   #211263

   Purpose

   Mammalian expression of full-length human ELP2. C-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   ELP2:M1-L826 (ELP2 Human)
   

   Use

   Tags

   GSSGGSSGVSGWRLFKKIS

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBiT3.1-N_UTP4:1-686

   Plasmid

   #211249

   Purpose

   Mammalian expression of full-length human UTP4. N-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   UTP4:M1-T686 (CIRH1A Human)
   

   Use

   Tags

   MVSGWRLFKKISGSSGGSSG

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBiT3.1-N_CDC40:1-579

   Plasmid

   #211029

   Purpose

   Mammalian expression of full-length human CDC40. N-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   CDC40:M1-D579 (CDC40 Human)
   

   Use

   Tags

   MVSGWRLFKKISGSSGGSSG

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBiT3.1-N_WDR5:1-334

   Plasmid

   #211036

   Purpose

   Mammalian expression of full-length human WDR5. N-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   WDR5:M1-C334 (WDR5 Human)
   

   Use

   Tags

   MVSGWRLFKKISGSSGGSSG

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHTC-HaloTag_BCL11A:1-773

   Plasmid

   #211015

   Purpose

   Mammalian expression of full-length human BCL11A. C-terminal HaloTag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   BCL11A:M1-F773 (BCL11A Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   isoform 2

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pNLF1-N_RBBP4:1-425

   Plasmid

   #211018

   Purpose

   Mammalian expression of full-length human RBBP4. N-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   RBBP4:M1-S425 (RBBP4 Human)
   

   Use

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNLF1-C_RBBP4:1-425

   Plasmid

   #211019

   Purpose

   Mammalian expression of full-length human RBBP4. C-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   RBBP4:M1-S425 (RBBP4 Human)
   

   Use

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBiT3.1-C_CDC40:1-579

   Plasmid

   #211032

   Purpose

   Mammalian expression of full-length human CDC40. C-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   CDC40:M1-D579 (CDC40 Human)
   

   Use

   Tags

   GSSGGSSGVSGWRLFKKIS

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNLF1-N_WDR5:1-334

    Plasmid

    #211025

    Purpose

    Mammalian expression of full-length human WDR5. N-terminal NanoLuc luciferase tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    WDR5:M1-C334 (WDR5 Human)
    

    Use

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNLF1-C_WDR5:1-334

    Plasmid

    #211026

    Purpose

    Mammalian expression of full-length human WDR5. C-terminal NanoLuc luciferase tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    WDR5:M1-C334 (WDR5 Human)
    

    Use

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNLF1-N_CCNE1:1-410

    Plasmid

    #211022

    Purpose

    Mammalian expression of full-length human CCNE1. N-terminal NanoLuc luciferase tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    CCNE1:M1-A410 (CCNE1 Human)
    

    Use

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBiT3.1-C_WDR5:1-334

    Plasmid

    #211044

    Purpose

    Mammalian expression of full-length human WDR5. C-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    WDR5:M1-C334 (WDR5 Human)
    

    Use

    Tags

    GSSGGSSGVSGWRLFKKIS

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHTC-HaloTag_SUZ12:1-739

    Plasmid

    #211010

    Purpose

    Mammalian expression of full-length human SUZ12. C-terminal HaloTag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    SUZ12:M1-L739 (SUZ12 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pHTN-HaloTag_SUZ12:1-739

    Plasmid

    #211009

    Purpose

    Mammalian expression of full-length human SUZ12. N-terminal HaloTag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    SUZ12:M1-L739 (SUZ12 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pHTN-HaloTag_BOP1:1-746

    Plasmid

    #211004

    Purpose

    Mammalian expression of full-length human BOP1. N-terminal HaloTag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    BOP1:M1-T746 (BOP1 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pHTN-HaloTag_C9orf72:1-481

    Plasmid

    #210986

    Purpose

    Mammalian expression of full-length human C9orf72. N-terminal HaloTag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    C9orf72:M1-F481 (C9orf72 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pHTC-HaloTag_CDC73:1-531

    Plasmid

    #210983

    Purpose

    Mammalian expression of full-length human CDC73. C-terminal HaloTag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    CDC73:M1-F531 (CDC73 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pNLF1-C_CCT5:1-541

    Plasmid

    #210979

    Purpose

    Mammalian expression of full-length human CCT5. C-terminal NanoLuc luciferase tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    CCT5:M1-E541 (CCT5 Human)
    

    Use

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHTC-HaloTag_ACTB:1-375

    Plasmid

    #210971

    Purpose

    Mammalian expression of full-length human ACTB. C-terminal HaloTag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    ACTB:M1-F375 (ACTB Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pHTC-HaloTag_DCAF12:1-453

    Plasmid

    #210967

    Purpose

    Mammalian expression of full-length human DCAF12. C-terminal HaloTag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    DCAF12:M1-S453 (DCAF12 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    R131Q natural variant (VAR_035322)

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pHTN-HaloTag_ACTB:1-375

    Plasmid

    #210970

    Purpose

    Mammalian expression of full-length human ACTB. N-terminal HaloTag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    ACTB:M1-F375 (ACTB Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pNLF1-N_CORO1A:1-461

    Plasmid

    #210964

    Purpose

    Mammalian expression of full-length human CORO1A. N-terminal NanoLuc luciferase tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    CORO1A:M1-K461 (CORO1A Human)
    

    Use

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pNLF1-C_CORO1A:1-461

    Plasmid

    #210965

    Purpose

    Mammalian expression of full-length human CORO1A. C-terminal NanoLuc luciferase tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    CORO1A:M1-K461 (CORO1A Human)
    

    Use

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pNLF1-N_DCAF15:1-600

    Plasmid

    #210958

    Purpose

    Mammalian expression of full-length human DCAF15. N-terminal NanoLuc luciferase tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    DCAF15:M1-L600 (DCAF15 Human)
    

    Use

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pNLF1-C_FBXW11:1-542

    Plasmid

    #210952

    Purpose

    Mammalian expression of full-length human FBXW11. C-terminal NanoLuc luciferase tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    FBXW11:M1-R542 (FBXW11 Human)
    

    Use

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBiT3.1-N_SKIC8:1-305

    Plasmid

    #211038

    Purpose

    Mammalian expression of full-length human SKIC8. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    SKIC8:M1-I305 (SKIC8 Human)
    

    Use

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBiT3.1-N_RBBP4:1-425

    Plasmid

    #211037

    Purpose

    Mammalian expression of full-length human RBBP4. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    RBBP4:M1-S425 (RBBP4 Human)
    

    Use

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAG-R2Tg_opt-3xFlag

    Plasmid

    #223251

    Purpose

    Mammalian expression of C-terminally Flag-tagged optimized R2Tg protein

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg protein_opt
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

    Plasmid

    #180280

    Purpose

    The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    CasMINI sgRNA (backbone) and BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. BPK1520

    Plasmid

    #65777

    Purpose

    Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    SpCas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAcGHLT-B-DDB1

    Plasmid

    #48638

    Purpose

    Expresses Full Lenght DDB1 in Insect Cells (ampR)

    Depositor

    Ning Zheng

    Article

    Li et al Cell. 2006 Jan 13;124(1):105-17.

    Insert

    DDB1 (DDB1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

33. pSin Helper

    Plasmid

    #127694

    Purpose

    Plasmid contains the Sindbis glycoprotein genes and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.

    Depositor

    Justin English, Bryan Roth

    Article

    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

    Insert

    Sindbis Glycoproteins
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

34. pTSin EGFP

    Plasmid

    #127695

    Purpose

    Plasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequence

    Depositor

    Justin English, Bryan Roth

    Article

    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pBTK503

    Plasmid

    #110616

    Purpose

    BTK assembled broad-host-range plasmid encoding GFP driven by CP25 promoter

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pSin Capsid

    Plasmid

    #127693

    Purpose

    Plasmid contains the Sindbis capsid gene and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.

    Depositor

    Justin English, Bryan Roth

    Article

    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

    Insert

    Sindbis Capsid
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. ZapLck biosensor

    Plasmid

    #131584

    Purpose

    Lck FRET Biosensor. To visualize Lck kinase activity in mammalian cells by FRET

    Depositor

    Yingxiao Wang

    Article

    Wan et al Sci Adv. 2019 Jun 19;5(6):eaau2001. doi:

    Insert

    ZapLck biosensor
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGL2- Empty control -SV40-Luc

    Plasmid

    #26280

    Purpose

    Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.

    Depositor

    Rudolf Jaenisch

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSRE SSG

    Plasmid

    #127692

    Purpose

    Plasmid expresses the Sindbis Structural Genome (SSG) via the serum response element (SRE) promoter. See Resource Information section for annotated plasmid sequence.

    Depositor

    Justin English, Bryan Roth

    Article

    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

    Insert

    Sindbis Structural Genome
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. MSP1673

    Plasmid

    #65769

    Purpose

    Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (x2)

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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41. IVT-donor_opt(R2Tg)-GFP

    Plasmid

    #223246

    Purpose

    In vitro transcription of the optimized R2Tg RNA donor

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg donor_opt
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. BPK764

    Plasmid

    #65767

    Purpose

    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (x2)

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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43. pDIRECT_22A

    Plasmid

    #91133

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:AtCas9 + AtU6:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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44. pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2

    Plasmid

    #164510

    Purpose

    Green glucose sensor with mRuby2 fusion, cytosolic

    Depositor

    Loren Looger

    Article

    Keller et al bioRxiv (2019) 571422; doi: https://doi.

    Has Service

    AAV9

    Insert

    (Cyto)iGlucoSnFR-mRuby2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Synthetic Construct

    Promoter

    Synapsin

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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45. pAAV-CMV-mCherry-NLS

    Plasmid

    #165442

    Purpose

    Expresses nuclear mCherry from CMV promoter

    Depositor

    Esteban Engel

    Article

    Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

    Insert

    mCherry-NLS
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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46. mCherry-pCAG-donor(R2Tg)-GFP(intron)

    Plasmid

    #223248

    Purpose

    Mammalian expression of the R2Tg RNA donor

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg donor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. Pet22b-mEGFP

    Plasmid

    #166439

    Purpose

    Bacterial expression of 6x His tagged mEGFP

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mEGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pBTK620

    Plasmid

    #110620

    Purpose

    BTK assembled broad-host-range plasmid encoding GFP driven by CP32 promoter

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 22, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

49. pBTK621

    Plasmid

    #110621

    Purpose

    BTK assembled broad-host-range plasmid encoding GFP driven by CP6 promoter

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 21, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

50. pBTK619

    Plasmid

    #110619

    Purpose

    BTK assembled broad-host-range plasmid encoding GFP driven by CP12b promoter

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge