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pFS_0379_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_3

Plasmid

#116991

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 3, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0346_pET30-RT-CRISPR_Bacteroides_fragilis_strain_S14_ARRAY_1_RC

Plasmid

#116958

Purpose

Expresses BfRT-Cas1-Cas2 under pT7lac promoter, encodes BfCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Bacteroides fragilis strain S14 RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0365_pET30-RT-CRISPR_Desulfarculus_baarsii_DSM_2075_ARRAY_2

Plasmid

#116977

Purpose

Expresses DbRT-Cas1-Cas2 under pT7lac promoter, encodes DbCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Desulfarculus baarsii DSM 2075 RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0364_pET30-RT-CRISPR_Desulfarculus_baarsii_DSM_2075_ARRAY_1_RC

Plasmid

#116976

Purpose

Expresses DbRT-Cas1-Cas2 under pT7lac promoter, encodes DbCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Desulfarculus baarsii DSM 2075 RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0345_pET30-RT-CRISPR_Bacteroides_fragilis_strain_S14_ARRAY_1

Plasmid

#116957

Purpose

Expresses BfRT-Cas1-Cas2 under pT7lac promoter, encodes BfCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Bacteroides fragilis strain S14 RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0377_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_2

Plasmid

#116989

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0378_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_2_RC

Plasmid

#116990

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 2 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0375_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_1

Plasmid

#116987

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
SYNJ1-145_pCSdest

Plasmid

#53810

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

SYNJ1-145 (SYNJ1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
CMYA5/pDEST15

Plasmid

#98106

Purpose

Express CMYA5 (aa 4039 – 4069) with a N-terminal GST tag in bacteria

Depositor

Rita Shiang

Article

Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

Insert

CMYA5 (CMYA5 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
CMYA5T2/pDEST17

Plasmid

#98118

Purpose

Express CMYA5 (aa 4039 – 4069) Leu allele (4063) witha N-terminal 6x-His tag in bacteria

Depositor

Rita Shiang

Article

Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

Insert

CMYA5 (CMYA5 Human)

Use

Tags

6X-His

Expression

Bacterial

Mutation

rs10043986 (NP_705838.3:p.Pro4063Leu)

Promoter

Available since

Aug. 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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CMYA5/pDEST17

Plasmid

#98115

Purpose

Express CMYA5 (aa 4039 – 4069) with a N-terminal 6x-His tag in bacteria

Depositor

Rita Shiang

Article

Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

Insert

CMYA5 (CMYA5 Human)

Use

Tags

6X-His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBW755_pCAG-pDest-U5-UX

Plasmid

#89507

Purpose

pCAG destination vector (U5-UX)

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW1716_hU6-pDest-U1-UX

Plasmid

#87579

Purpose

hU6 destination vector

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST27-WFS1

Plasmid

#79276

Purpose

Mammalian expression of WFS1 with an N-terminal GST

Depositor

Rita Shiang

Insert

WFS1 (WFS1 Human)

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 8, 2016

Availability

Industry, Academic Institutions, and Nonprofits
POLN delP_pDEST17

Plasmid

#64886

Purpose

bacterial expression of human POLN with a short truncation of the C-terminal proline-rich tail

Depositor

Richard Wood

Article

Takata et al J Biol Chem. 2006 Aug 18;281(33):23445-5

Insert

POLN (POLN Human)

Use

Tags

Flag and His

Expression

Bacterial

Mutation

with a short truncation of the C-terminal proline…

Promoter

T7

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST47-ARL2

Plasmid

#67459

Purpose

Mammalian expression of hArl2 with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL2 (ARL2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL4

Plasmid

#67461

Purpose

Mammalian expression of hArl4 with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4 (ARL4A Human)

Use

Tags

Expression

Mammalian

Mutation

bp 105 A to T; silent mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL5A

Plasmid

#67462

Purpose

Mammalian expression of hArl5 with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL5A (ARL5A Human)

Use

Tags

Expression

Mammalian

Mutation

bp 70 C to T; silent mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL5B

Plasmid

#67463

Purpose

Mammalian expression of hArl5b with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL5B (ARL5B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only