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We narrowed to 11,127 results for: 158

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Showing: 10601 - 10620 of 11127 results

  5. pLV-puro-EF1A-3XFLAG-hASB8_ANK3-4

    Plasmid
    #242468
    Purpose
    Expresses human ASB8 protein with an N-terminal 3xFLAG tag along with a puromycin selection marker. The ANK3-4 mutations disrupt XPO1 binding.
    Depositor
    Dirk Daelemans
    Article
    Wing et al Nat Chem Biol. 2025 Dec;21(12):2002-2013
    Insert
    ASB8 (ASB8 Human)
    Use
    Lentiviral
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    ANK3-4 = R87A - E95A - K96A - W124G - K128G
    Promoter
    EF1A
    Available Since
    Nov. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    242468-plasmid-map-sequence-id-488467

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  8. pLCKO-CMV-cMyc-UBC_K7R-IRES-Blasti

    Plasmid
    #242463
    Purpose
    Expresses human UBC protein with an N-terminal cMyc tag enabling UBC pull down along with a blasticidin selection marker. The K7R mutations prevent ubiquitin chain elongation.
    Depositor
    Dirk Daelemans
    Article
    Wing et al Nat Chem Biol. 2025 Dec;21(12):2002-2013
    Insert
    UBC (UBC Human)
    Use
    Lentiviral
    Tags
    cMyc
    Expression
    Mammalian
    Mutation
    UBC-K7R
    Promoter
    CMV
    Available Since
    Nov. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    242463-plasmid-map-sequence-id-488455

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  10. pBI121::chitRatioGFP:LAAQ

    Plasmid
    #240451
    Purpose
    Expression of indicator protein fusion (modified ratiometric pHluorin & low affinity Aequorin) for monitoring calcium concentrations and pH in cell walls of higher plants. See Resource Information.
    Depositor
    Christoph Plieth
    Article
    Gao et al Plant Physiol. 2004 Mar;134(3):898-908.
    Insert
    Fusion of chitinase signal, soluble modified ratiometric pHluorin and low affinity Aequorin (D119A)
    Expression
    Bacterial and Plant
    Mutation
    Ratiometric GFP (ratiometric pHluorin) (PMID 9671…
    Promoter
    Cauliflower mosaic virus 35S promoter (GenBank AJ…
    Available Since
    Sept. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    240451-plasmid-map-sequence-id-485323

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  15. pRCA378 - pBA904 Puro-T2A-GFP CD45 CRISPRa guide 3 (pRCA360 backbone)

    Plasmid
    #238169
    Purpose
    Lentiviral CRISPR guide vector expressing PTPRC targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
    Depositor
    Thomas Norman
    Article
    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415
    Insert
    CD45 sgRNA CRISPRa (PTPRC Synthetic, Human)
    Use
    CRISPR and Lentiviral
    Tags
    GFP
    Available Since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    238169-plasmid-map-sequence-id-474451

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  19. Click editor utilizing mSA for template recruitment and ePhi29 DNA polymerase - pCMV-T7-mSA-nCas9-ePhi29 (JO1398)

    Plasmid
    #217803
    Purpose
    Variant CE1 construct with mSA for template recruitment and ePhi29(D169A) DNA pol, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    mSA-linker-SV40NLS-nSpCas9-BPNLS-ePhi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); Phi29(D169A/M8R/V51A/M97T/G197D/E…
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217803-plasmid-map-sequence-id-432890

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  20. Unfused click editor construct; mSA-ePhi29 - pCMV-T7-mSA-Phi29 (JO1411)

    Plasmid
    #217807
    Purpose
    Unfused click editor construct expressing mSA-ePhi29(D169A), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    mSA-linker-SV40NLS-ePhi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Phi29(D169A/M8R/V51A/M97T/G197D/E221K/Q497P/K512E…
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217807-plasmid-map-sequence-id-432906

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Showing: 10601 - 10620 of 11127 results