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pGWB515_35S-HA-(ΔTP)DM3(Col-0) D332R Q345R

Plasmid

#229721

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Mol Cell. 2025 Jul 17;85(14):2776-2795.e

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-52 were deleted, D332R Q345R

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB515_35S-HA-(ΔTP)DM3(Col-0) S214A

Plasmid

#229723

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Mol Cell. 2025 Jul 17;85(14):2776-2795.e

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-52 were deleted, S214A

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB515_35S-HA-(ΔTP)DM3(Col-0) T359A

Plasmid

#229727

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Mol Cell. 2025 Jul 17;85(14):2776-2795.e

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-52 were deleted, T359A

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB512_35S-FLAG-(ΔTP)DM3(Hh-0)

Plasmid

#229728

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Mol Cell. 2025 Jul 17;85(14):2776-2795.e

Insert

DM3(Hh-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-52 were deleted, 511-515 were deleted

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB512_35S-FLAG-(ΔTP)DM3(Hh-0) ΔC

Plasmid

#229730

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Mol Cell. 2025 Jul 17;85(14):2776-2795.e

Insert

DM3(Hh-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-52 were deleted, 511-515 were deleted

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB512_35S-FLAG-(ΔTP)DM3(Col-0) ΔC

Plasmid

#229731

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Mol Cell. 2025 Jul 17;85(14):2776-2795.e

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-52 were deleted, 511-515 were deleted

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB512_35S-FLAG-DM3(Col-0)68-510

Plasmid

#229733

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Mol Cell. 2025 Jul 17;85(14):2776-2795.e

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-67 were deleted, 511-515 were deleted

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB515_35S-HA-(ΔTP)DM3(Col-0) Y460E V484E

Plasmid

#229715

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Mol Cell. 2025 Jul 17;85(14):2776-2795.e

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-52 were deleted

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB515_35S-HA-(ΔTP)DM3(Col-0) D332A Q345A N349A

Plasmid

#229716

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Mol Cell. 2025 Jul 17;85(14):2776-2795.e

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-52 were deleted, D332A Q345A N349A

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hRIPK3_1k)-PGKpuro2ABFP-W

Plasmid

#208424

Purpose

Lentiviral gRNA plasmid targeting human RIPK3 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

RIPK3 (Ripk3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hMTOR_1k)-PGKpuro2ABFP-W

Plasmid

#208429

Purpose

Lentiviral gRNA plasmid targeting human MTOR gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Heliyon. 2024 Oct 22;10(21):e39677. doi:

Insert

MTOR (MTOR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b-ITGB1(WT)

Plasmid

#215450

Purpose

Gateway entry vector with integrin beta1 wild-type (WT)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Gateway entry vector

Mutation

Silent mutations added to disrupt shRNA binding a…

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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sgYAP-R-1

Plasmid

#229445

Purpose

knockout of YAP1

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgYAP-R-1 (YAP1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6;bU6;EFS

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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Cdk13_intron3_sg3_pX458

Plasmid

#127338

Purpose

sgRNA that cuts within intron 3 of mouse CDK13 genomic locus- guide #3

Depositor

Phil Sharp

Article

Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

Insert

Mouse Cdk13 Intron 3 sgRNA

Use

CRISPR and Mouse Targeting

Promoter

U6 Promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2a_(CJT91)

Plasmid

#226991

Purpose

pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2a must be used with gRNA 1a or 1b

Depositor

Benjamin Kleinstiver

Article

Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

Insert

SpCas9 gRNA 2a to create OPA1-del_exon5

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2b_(CJT93)

Plasmid

#226992

Purpose

pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2b must be used with gRNA 1a or 1b

Depositor

Benjamin Kleinstiver

Article

Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

Insert

SpCas9 gRNA 2b to create OPA1-del_exon5

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pX459_TELO2_gRNA

Plasmid

#224379

Purpose

Targets TELO2 for dTAG N terminal knock-in cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

TELO2 (TELO2 Human)

Use

CRISPR

Expression

Bacterial and Mammalian

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pKM-U6-reRNA-hsRUNX

Plasmid

#205639

Purpose

reRNA guide targeting Homo sapiens RUNX

Depositor

Gerald Schwank

Article

Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

Insert

reRNA-hsRUNX (RUNX1 Human)

Expression

Mammalian

Promoter

U6

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 Q280* K418* full-length

Plasmid

#221406

Purpose

Mammalian expression of human integrin beta1 Q280* K418* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 Q280* K418* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 N391* N562* full-length

Plasmid

#221407

Purpose

Mammalian expression of human integrin beta1 N391* N562* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 N391* N562* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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