We narrowed to 35,397 results for: lat

Showing: 10741 - 10760 of 35397 results

Results per page:

Sort results by:

pTet-O-NEUROG1-T2A-PuroR

Plasmid

#162339

Purpose

Lentiviral expression of NEUROG1 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

NEUROG1 (NEUROG1 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTet-O-NEUROG3-T2A-PuroR

Plasmid

#162341

Purpose

Lentiviral expression of NEUROG3 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

NEUROG3 (NEUROG3 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET32a-Spike-403-505

Plasmid

#165095

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 spike protein site 403-505 (S SARS-CoV2)

Expression

Bacterial

Available Since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGIPz-PD-L1 4NQ

Plasmid

#121487

Purpose

Expression of human PD-L1 4NQ with PD-L1 shRNA

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Inserts

PD-L1 4NQ
hPD-L1 shRNA

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

N35Q, N192Q, N200Q and N219Q

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX4T1-Rpb4/7

Plasmid

#138484

Purpose

Expresses GST-tagged Rpb4 and His-tagged Rpb7 in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Inserts

Rpb4 (POLR2D Human)
Rpb7 (POLR2G Human)

Tags

6xHis and GST

Expression

Bacterial

Promoter

tac

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMYs-MFN2-IRES-mitoRFP

Plasmid

#121997

Purpose

Expression of MFN2 and mitoRFP in mammalial cells

Depositor

Qing Deng

Article

Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

Insert

MFN2-IRES-mitoRFP (MFN2 Human)

Use

Retroviral

Mutation

silent Q386Q Q387Q V388V Y389Y C390C E391E E392E

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pKnockinDonor-humanCTCF-miniAID-mClover3

Plasmid

#136883

Purpose

knockin donor vector of miniAID-mClover3 to C-terminus of endogenous human CTCF

Depositor

Chunliang Li

Article

Hyle et al Nucleic Acids Res. 2019 Jul 26;47(13):66

Insert

CTCF-knockin-homology arm-miniAID-mClover3

Tags

miniAID-mClover3

Expression

Mammalian

Promoter

no promoter

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRepair-SGFP2-CTNNB1

Plasmid

#153430

Purpose

Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SGFP2

Depositor

Renee van Amerongen

Article

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)

Use

CRISPR; Hdr donor template

Tags

SGFP2

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT-TO-FH-Nsp13_GC3opt

Plasmid

#157715

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp13_GC3opt (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCyCEN_Lisp2mCherry_hsp70_GFP

Plasmid

#137169

Purpose

Dual fluorescent reporter for liver stage expression in Plasmodium cynomolgi

Depositor

Clemens Kocken

Article

Voorberg-van der Wel et al Commun Biol. 2020 Jan 3;3:7. doi: 10.103

Inserts

mCherry
Nanoluc
dihydrofolate reductase
Green Fluorescent Protein

Use

Unspecified

Tags

T2A

Promoter

P. cynomolgi hsp70 and P. cynomolgi lisp2

Available Since

May 11, 2020

Availability

Academic Institutions and Nonprofits only
FUG-T2A-Pten

Plasmid

#140738

Purpose

Lentiviral vector to overexpress human PTEN and GFP

Depositor

Bryan Luikart

Article

Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

Insert

GFP-PTA-PTEN (PTEN Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Promoter

hUbiC

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV6- Entry-RPL26DC-DDK

Plasmid

#133867

Purpose

Expression of C-terminally FLAG-tagged human RPL26 mutant lacking the C-terminal UFMylation sites

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

RPL26DC-myc-FLAG (RPL26 Human)

Tags

Myc-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-W

Plasmid

#105039

Purpose

Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Tsc2 (Tsc2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWJB30

Plasmid

#126505

Purpose

Salmonella typhimurium diguanylate cyclase AdrA cloned as AdrA-8xHis in pBAD30

Depositor

Ute Romling

Article

Simm et al Mol Microbiol. 2004 Aug;53(4):1123-34. d

Insert

adrA-8xHis

Tags

8xHis

Expression

Bacterial

Promoter

PBAD30

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLN484 (FuGW-S(cMYC)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe)

Plasmid

#105192

Purpose

Module 1 - synthetic promoter cMYC drives self-inhibiting GAD expression (see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(cMYC)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV CAG ChR2 E123T T159C 2A tDimer

Plasmid

#85399

Purpose

High efficiency channelrhodopsin with cytomegalovirus enhancer fused to chicken β-actin (CAG) promoter

Depositor

Thomas Oertner

Article

Berndt et al Proc Natl Acad Sci U S A. 2011 May 3;108

Inserts

Channelrhodopsin-2
red fluorescent protein

Use

AAV

Expression

Mammalian

Mutation

E123T , T159C

Promoter

CAG and ribosomal skip sequence 2A

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV2_hSyn_Phobos_Citrine

Plasmid

#98216

Purpose

Blue-shifted artificial anion conducting channelrhodopsin (aACR). Activation max 466 nm; off-kinetics 10 ms. Codon optimized for mammalian expression.

Depositor

Simon Wiegert

Article

Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

Insert

Synthetic construct Phobos gene

Use

AAV

Tags

Citrine

Expression

Mammalian

Mutation

T59S, E83N, E90Q, E101S, V117R, E123S, T159G, G16…

Promoter

human synapsin

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1α1.1-FLEX-rc [Chronos-GFP]

Plasmid

#84485

Purpose

AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

EF1α, 1.1 kb long (short version)

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA 3.2/V5-DEST NET1A V5 WT

Plasmid

#69817

Purpose

Expresses NET1A-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

NET1A (NET1 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C3-PLK4-S285A/T289A-3xFLAG

Plasmid

#69841

Purpose

This plasmid encodes PLK4 isoform 1 carrying S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Mutation

S285A/T289A

Promoter

CMV

Available Since

Dec. 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge