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pQE30-His-RF3

Plasmid

#124131

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Release factor 3 (prfC Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMEH380 VSV-G HA

Plasmid

#168222

Purpose

Mammalian expression of vesicular stomatitis virus glycoprotein fused to a C-terminal HA tag

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

VSV-G

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_Ado1.0

Plasmid

#153291

Purpose

Express the adenosine sensor GRAB_Ado1.0 in Cre positive cells

Depositor

Yulong Li

Article

Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

Insert

Adenosine sensor GRAB_Ado1.0

Use

AAV

Promoter

EFS

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pSIL-eGFP

Plasmid

#52675

Purpose

shRNA silencing plasmid with eGFP transfection marker

Depositor

Johannes Hell

Article

Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

Type

Empty backbone

Use

RNAi

Tags

CMV-EGFP

Promoter

CMV and U6

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pQE60-EFTs-His

Plasmid

#124128

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Elongation factor Ts (tsf Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-MCS-Ds

Plasmid

#102416

Purpose

Empty vector with a multiple cloning site flanked by Ds integration elements

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pT2SK

Plasmid

#59383

Purpose

Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a kanamycin resistance gene.

Depositor

Shelley Copley

Article

Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

Insert

TT-ISceI-kan

Use

Template

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pH2B-mTFP1

Plasmid

#244036

Purpose

anchoring mTFP1 to the chromatin/nucleus

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

H2B-mTFP1

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Tat HA (P#29)

Plasmid

#14654

Depositor

Matija Peterlin

Article

Cujec et al Genes Dev. 1997 Oct 15. 11(20):2645-57.

Insert

Tat (tat HIV)

Tags

HA

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pCS2.aBTX

Plasmid

#122257

Purpose

Express membrane-tethered alpha-bungarotoxin in Danio rerio

Depositor

Loren Looger

Article

Wan et al Cell. 2019 Oct 3;179(2):355-372.e23. doi

Insert

alpha-bungarotoxin

Use

Synthetic Biology

Tags

FLAG

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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Double UP mClover3 to mScarlet

Plasmid

#120262

Purpose

Natively produces mClover3, in the presence of Cre will be recombined to produce mScarlet.

Depositor

Erik Dent

Article

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Insert

mClover3/mScarletP2A-mcs

Expression

Mammalian

Mutation

Removed Not1 site in mScarlet

Promoter

CAG

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pQE60-RRF-His

Plasmid

#124132

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Ribosome recycling factor (frr Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pPM180

Plasmid

#64822

Purpose

GUS overexpression in planta

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

GUS

Expression

Plant

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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dCas9-5xGCN4-P2A-BFP

Plasmid

#184438

Purpose

dCas9 fused with 5 copies of GCN4 epitope

Depositor

Rhys Allan

Article

Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

Insert

dCas9-5xGCN4

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pRD119 (PCNA GFP)

Plasmid

#14822

Depositor

Robert Duronio

Article

Thacker et al Curr Biol. 2003 Jan 8. 13(1):53-8.

Insert

PCNA-GFP (PCNA Fly)

Tags

GFP

Expression

Bacterial

Available Since

July 30, 2007

Availability

Academic Institutions and Nonprofits only
pFRT_TO_FlagHA_USP10_C424A

Plasmid

#127103

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

USP10 (USP10 Human)

Expression

Mammalian

Mutation

C424A

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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Double UP mScarlet to mNeon

Plasmid

#125139

Purpose

Natively produces mScarlet, in the presence of Cre will be recombined to produce mNeon.

Depositor

Erik Dent

Article

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Type

Empty backbone

Expression

Mammalian

Mutation

Removed Not1 site in mScarlet

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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FKBP-eGFP-TAZ

Plasmid

#221213

Purpose

part A of TAZ-SPARK-OFF

Depositor

Xiaokun Shu

Article

Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

Insert

FKBP-eGFP-TAZ

Expression

Mammalian

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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FRP880_PACT1(-1-520)-LexA-ER-haB112-TCYC1

Plasmid

#58437

Purpose

Expresses LexA-ER-B112 in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

LexA-ER-B112

Expression

Yeast

Promoter

Act1 promoter (-520;1)

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pCX-V5-H7N9-NS1

Plasmid

#99022

Purpose

Mammalian expression of NS1

Depositor

Honglin Chen

Article

Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

Insert

NS1

Tags

V5

Expression

Mammalian

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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