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We narrowed to 57,324 results for: plasmid

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Showing: 10741 - 10760 of 57324 results

  4. pCOTS-pyl-GFP(35TAG)

    Plasmid
    #92047
    Purpose
    This is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.
    Depositor
    Lital Alfonta
    Publication
    Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/
    Inserts
    EGFP
    Pyrrolysyl tRNA(cua)
    Pyrrolysyl tRNA synthetase (methanosarcina mazei)
    Use
    Replicative expression plasmid for cyanobacteria …
    Tags
    Histag
    Mutation
    Tyrosine in position 35 of the GFP was mutated f…
    Promoter
    LeuP (native S. elongatus promoter), PpsbII, and …
    Available since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92047-plasmid-map-sequence-id-194905

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  5. pECT5

    Plasmid
    #75451
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt5 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Publication
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt5
    Expression
    Bacterial
    Promoter
    none
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75451-plasmid-map-sequence-id-146479

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  6. pST50Trc3-STRNDHFR

    Plasmid
    #63978
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Publication
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    Strep II (STR); TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    63978-plasmid-map-sequence-id-177384

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  7. pST50Trc1-HISDHFR

    Plasmid
    #63935
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Publication
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    63935-plasmid-map-sequence-id-177341

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  8. KIF2C

    Plasmid
    #25551
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    KIF2C (KIF2C Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article

  9. RNF168

    Plasmid
    #25298
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    rnf168.0001.0113 (RNF168 Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 16, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article

  10. SETDB1

    Plasmid
    #25240
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SETDB1, SETDB1_16 (SETDB1 Human)
    Tags
    6xHis-TEV and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. pELPS-eDHFR-YFP-T2A-Renilla

    Plasmid
    #193253
    Purpose
    Triple reporter plasmid in pELPS with eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
    Depositor
    Mark Sellmyer
    Publication
    Sellmyer et al Mol Ther. 2020 Jan 8;28(1):42-51. doi: 1
    Insert
    The eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
    Use
    Lentiviral
    Tags
    Renilla luciferase (rLuc) and yellow fluorescent …
    Mutation
    None
    Promoter
    EF1a
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    193253-plasmid-map-sequence-id-383064

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  14. pLenti-dCas9-KRAB-gRNA-TRE-blast

    Plasmid
    #201152
    Purpose
    Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.
    Depositor
    Moshe Szyf
    Inserts
    dCas9-KRAB
    gRNA-TRE
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Mutation
    gRNA sequence: TACGTTCTCTATCACTGAT
    Available since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    201152-plasmid-map-sequence-id-401799

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  15. pmCellFree_KA1_SARS-COV-2_ORF3a

    Plasmid
    #169390
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF3a with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF3a (ORF3a SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169390-plasmid-map-sequence-id-336867

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  16. pmCellFree_KA1_SARS-COV-2_ORF9b

    Plasmid
    #169396
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF9bwith N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF9b
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    169396-plasmid-map-sequence-id-335426

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  17. pmCellFree_KA1_SARS-COV-2_E

    Plasmid
    #169399
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus E with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _E (E SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    169399-plasmid-map-sequence-id-335422

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  18. pmCellFree_KA1_SARS-COV-2_NSP5

    Plasmid
    #169380
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP5 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP5
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available since
    June 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    169380-plasmid-map-sequence-id-335068

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  19. pmCellFree_KA1_SARS-COV-2_NSP2

    Plasmid
    #169377
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP2 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP2
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Promoter
    T7- CMV/CAG
    Available since
    June 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    169377-plasmid-map-sequence-id-335058

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Showing: 10741 - 10760 of 57324 results