Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 16,183 results for: nes

Clear keywords
Showing: 10761 - 10780 of 16183 results

  4. AP70_pU.CAG.Cas9-D10A-K848A-R1060A.rBGpA

    Plasmid
    #199254
    Purpose
    Expression construct encoding the high specificity SpCas9-KARA-D10A nickase
    Depositor
    Manuel Goncalves
    Publication
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Insert
    High specificity S. pyogenes Cas9-D10A-KARA nickase
    Use
    CRISPR
    Tags
    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A K848A R1060A
    Promoter
    CAG promoter
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199254-plasmid-map-sequence-id-391656

    Enlarge

  9. pJCC_163 SpCas9 K233A/K234A/K253A/K263A

    Plasmid
    #179527
    Purpose
    For bacterial expression of SpCas9 K233A/K234A/K253A/K263A (helix-rolling basic patch mutant) with an N-terminal His-MBP tag
    Depositor
    Jennifer Doudna
    Publication
    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-
    Insert
    SpCas9 K233A/K234A/K253A/K263A
    Tags
    10X His, TEV protease cleavage site, and maltose-…
    Expression
    Bacterial
    Mutation
    changed lysines 233, 234, 253, and 263 to alanine
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    179527-plasmid-map-sequence-id-351733

    Enlarge

  13. pJSC119 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant

    Plasmid
    #101207
    Purpose
    Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant N497A/R661A/Q695A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    N497A, R661A and Q695A
    Promoter
    T7
    Available since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101207-plasmid-map-sequence-id-198129

    Enlarge

  16. pJSC077 - Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant

    Plasmid
    #101214
    Purpose
    Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S355C/S867C/K855A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S355C, S867C and K855A
    Promoter
    T7
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    101214-plasmid-map-sequence-id-197223

    Enlarge

  17. pJSC115 - Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant

    Plasmid
    #101210
    Purpose
    Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A/Q926A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S355C, S867C, N497A, R661A, Q695A an…
    Promoter
    T7
    Available since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101210-plasmid-map-sequence-id-198133

    Enlarge

  18. pJSC038 - Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant

    Plasmid
    #101203
    Purpose
    Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S355C/S867C/M1–N497,GGS,V713–D1368
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S355C, S867C, M1-N497, GGS, V713-D13…
    Promoter
    T7
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    101203-plasmid-map-sequence-id-197572

    Enlarge

  19. pJSC131 - Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant

    Plasmid
    #101212
    Purpose
    Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S701C/S960C/N497A/R661A/Q695A/Q926A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S701C, S960C, N497A, R661A, Q695A an…
    Promoter
    T7
    Available since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    101212-plasmid-map-sequence-id-197040

    Enlarge

  20. MB 98w3 ttrSR(m13)-Bxb1_P7-bARGSer

    Plasmid
    #232473
    Purpose
    Optimized tetrathionate sensor with recombinase switch and acoustic reporter genes (bARGSer)
    Depositor
    Mikhail Shapiro
    Publication
    Buss et al Nat Commun. 2025 Aug 25;16(1):7931. doi:
    Inserts
    ttrS
    ttrR
    PttrB185-269
    bARGSer
    AxeTxe
    Bxb1 integrase
    Tags
    ssrA degradation tag
    Expression
    Bacterial
    Mutation
    the gene Ser39006_001280 was deleted from the clu…
    Promoter
    Constitutive (TTGATAGCTAGCTCAGTCCTAGGTATTGTGCTAGC…
    Available since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    232473-plasmid-map-sequence-id-467800

    Enlarge

Showing: 10761 - 10780 of 16183 results